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  4. Recombinant Rabbit Cardiac phospholamban (PLN), partial

Recombinant Rabbit Cardiac phospholamban (PLN), partial

  • 中文名称：
    小圆锥花果PLN重组蛋白
  • 货号：
    CSB-YP018198RB
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小圆锥花果PLN重组蛋白
  • 货号：
    CSB-EP018198RB
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小圆锥花果PLN重组蛋白
  • 货号：
    CSB-EP018198RB-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小圆锥花果PLN重组蛋白
  • 货号：
    CSB-BP018198RB
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小圆锥花果PLN重组蛋白
  • 货号：
    CSB-MP018198RB
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    PLN; Cardiac phospholamban; PLB
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • 基因功能参考文献：
    1. Acute expression of R9C mutation of phospholamban in cardiomyocytes was positively inotropic/lusitropic. PMID: 25593317
    2. Ca(2+) and PLB phosphorylation relieve SERCA-PLB inhibition by distinct mechanisms, but both are achieved primarily by structural changes within the SERCA-PLB complex, not by dissociation of that complex. PMID: 24813991
    3. Phosphorylated phospholamban stabilizes a unique conformation of SERCA that is characterized by a compact architecture. PMID: 24138857
    4. Data suggest that phospholamban PLN's conformational equilibrium is central to maintain sarcoplasmic reticulum Ca(2+)-ATPase SERCA's apparent Ca(2+) affinity within a physiological window. PMID: 24101520
    5. demonstrate that the role of Arg(9) in phospholamban function is multifaceted: it is important for inhibition of SERCA, it increases the efficiency of phosphorylation, and it is critical for protein kinase A recognition PMID: 22707725
    6. The interaction energies between the N-terminal helix of phospholamban and different POPC lipid/cholesterol bilayers quantitatively confirm its stronger interaction with a higher cholesterol-containing membrane. PMID: 21993332
    7. The lipid bilayer composition influences the regulation of SERCA by PLN. PMID: 21992175
    8. Phospholamban overexpression in rabbit ventricular myocytes does not alter sarcoplasmic reticulum Ca transport. PMID: 19112098

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Sarcoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Membrane; Single-pass membrane protein.
  • 蛋白家族：
    Phospholamban family
  • 组织特异性：
    Heart.
  • 数据库链接：

    KEGG: ocu:100009299

    STRING: 9986.ENSOCUP00000011917

    UniGene: Ocu.3266