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  4. Recombinant Pseudomonas putida Ribosomal RNA large subunit methyltransferase N (rlmN)

Recombinant Pseudomonas putida Ribosomal RNA large subunit methyltransferase N (rlmN)

  • 中文名称：
    恶臭假单胞菌rlmN重组蛋白
  • 货号：
    CSB-YP543068FGC
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    恶臭假单胞菌rlmN重组蛋白
  • 货号：
    CSB-EP543068FGC
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    恶臭假单胞菌rlmN重组蛋白
  • 货号：
    CSB-EP543068FGC-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    恶臭假单胞菌rlmN重组蛋白
  • 货号：
    CSB-BP543068FGC
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    恶臭假单胞菌rlmN重组蛋白
  • 货号：
    CSB-MP543068FGC
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rlmN
  • Uniprot No.：
  • 别名：
    rlmN; PputW619_4328; Dual-specificity RNA methyltransferase RlmN; EC 2.1.1.192; 23S rRNA; adenine(2503)-C(2))-methyltransferase; 23S rRNA m2A2503 methyltransferase; Ribosomal RNA large subunit methyltransferase N; tRNA; adenine(37)-C(2))-methyltransferase; tRNA m2A37 methyltransferase
  • 种属：
    Pseudomonas putida (strain W619)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-381
  • 氨基酸序列
    MTTSTGKINL LGLTQPEMEQ FFDSIGEKRF RAGQVMKWIH HFGVDDFAAM TNVGKALREK LEAVAEIRPP EVVSEDISAD GTRKWVIRVA SGSCVETVYI PTDDRGTLCV SSQAGCALDC SFCSTGKQGF NSNLTAAEVI GQVWLANKSF GTVPAKIDRA ITNVVMMGMG EPLLNFDNVI AAMKIMMDDL GYGISKRRVT LSTSGVVPMI DELAKHIDVS LALSLHAPND ELRNQLVPIN KKYPLKMLLE SCMGYMATLG GKRVLTVEYT LLKDVNDQPE HAAQMIELLR DVPCKINLIP FNPFPHSGYE RPSNNAIRRF QDLLHHGGFN VTTRTTRGDD IDAACGQLVG QVNDRTRRSE RYIAVRQLSA DAELQDSAVR H
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Radical SAM superfamily, RlmN family
  • 数据库链接：

    KEGG: ppw:PputW619_4328

    STRING: 390235.PputW619_4328