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  4. Recombinant Pseudomonas aeruginosa RNA polymerase sigma factor rpoS (rpoS)

Recombinant Pseudomonas aeruginosa RNA polymerase sigma factor rpoS (rpoS)

  • 中文名称：
    铜绿假单胞菌rpoS重组蛋白
  • 货号：
    CSB-YP338271EZX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    铜绿假单胞菌rpoS重组蛋白
  • 货号：
    CSB-EP338271EZX
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    铜绿假单胞菌rpoS重组蛋白
  • 货号：
    CSB-EP338271EZX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    铜绿假单胞菌rpoS重组蛋白
  • 货号：
    CSB-BP338271EZX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    铜绿假单胞菌rpoS重组蛋白
  • 货号：
    CSB-MP338271EZX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rpoS
  • Uniprot No.：
  • 别名：
    rpoS; PA3622; RNA polymerase sigma factor RpoS; Sigma S; Sigma-38
  • 种属：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-334
  • 氨基酸序列
    MALKKEGPEF DHDDEVLLLE PGIMLDESSA DEQPSPRATP KATTSFSSKQ HKHIDYTRAL DATQLYLNEI GFSPLLTPEE EVHFARLAQK GDPAGRKRMI ESNLRLVVKI ARRYVNRGLS LLDLIEEGNL GLIRAVEKFD PERGFRFSTY ATWWIRQTIE RAIMNQTRTI RLPIHVVKEL NVYLRAAREL THKLDHEPSP EEIANLLEKP VAEVKRMLGL NERVTSVDVS LGPDSDKTLL DTLTDDRPTD PCELLQDDDL SESIDQWLTE LTDKQREVVI RRFGLRGHES STLEEVGQEI GLTRERVRQI QVEALKRLRE ILEKNGLSSD ALFQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response.
  • 基因功能参考文献：
    1. Pyocyanin production by mutant DeltarpoS increased dramatically in comparison with the wild-type strain PAO1. PMID: 29750486
    2. RpoS transcriptionally regulates polysaccharide psl expression. PMID: 20735777
    3. results indicate that rpoS gene is involved in tolerance to antibiotics in P. aeruginosa during the stationary phase and heat stress; however, under osmotic stress, tolerance to biapenem was not dependent on the rpoS gene PMID: 15621433
    4. A deletion-insertion mutation of alternative sigma factor RpoS eliminated Aer-2-mediated aerotaxis but not Aer-mediated aerotaxis. PMID: 15687221
    5. These results suggest that the Las QS system in Pseudomonas aeruginosa is involved in the development of ofloxacin tolerance, and the tolerance induced by the Las-system is regulated by rpoS gene. PMID: 19645822

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Sigma-70 factor family, RpoS subfamily
  • 数据库链接：

    KEGG: pae:PA3622

    STRING: 208964.PA3622