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  4. Recombinant Pseudomonas aeruginosa RNA polymerase sigma-H factor (algU)

Recombinant Pseudomonas aeruginosa RNA polymerase sigma-H factor (algU)

  • 货号：
    CSB-YP313274EZX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP313274EZX
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP313274EZX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP313274EZX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP313274EZX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    algU
  • Uniprot No.：
  • 别名：
    algU; algT; PA0762; RNA polymerase sigma-H factor; Sigma-30
  • 种属：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-193
  • 氨基酸序列
    MLTQEQDQQL VERVQRGDKR AFDLLVLKYQ HKILGLIVRF VHDAQEAQDV AQEAFIKAYR ALGNFRGDSA FYTWLYRIAI NTAKNHLVAR GRRPPDSDVT AEDAEFFEGD HALKDIESPE RAMLRDEIEA TVHQTIQQLP EDLRTALTLR EFEGLSYEDI ATVMQCPVGT VRSRIFRARE AIDKALQPLL REA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis.
  • 基因功能参考文献：
    1. In the current study, s mapped the mucE transcriptional start site, and determined that P(mucE) activity was dependent on AlgU. PMID: 24138584
    2. AlgT, AlgU and AlgO positively regulate alginate production. PMID: 22088575
    3. Data show that P. aeruginosa cultured in low shear modelled microgravity exhibit increased alginate production and upregulation of AlgU-controlled transcripts, including those encoding stress-related proteins. PMID: 20236169
    4. The algU mutant of P. aeruginosa PAO1 (PAOU) showed a dramatic impairment in biofilm formation under dynamic conditions; PAOU was defective both in cell attachment to glass and in development of robust, shear-resistant biofilms. PMID: 20348252
    5. Sigma 22 is controlled post-translationally by proteolysis and the sigma 22 regulon during cell wall stress. PMID: 19226327

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  • 蛋白家族：
    Sigma-70 factor family, ECF subfamily
  • 数据库链接：

    KEGG: pae:PA0762

    STRING: 208964.PA0762