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  4. Recombinant Pseudomonas aeruginosa Porin P (oprP)

Recombinant Pseudomonas aeruginosa Porin P (oprP)

  • 中文名称：
    铜绿假单胞菌oprP重组蛋白
  • 货号：
    CSB-YP361596EZX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    铜绿假单胞菌oprP重组蛋白
  • 货号：
    CSB-EP361596EZX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    铜绿假单胞菌oprP重组蛋白
  • 货号：
    CSB-BP361596EZX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    铜绿假单胞菌oprP重组蛋白
  • 货号：
    CSB-MP361596EZX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    oprP
  • Uniprot No.：
  • 别名：
    oprP; PA3279; Porin P; Outer membrane protein D1
  • 种属：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    30-440
  • 氨基酸序列
    G TVTTDGADIV IKTKGGLEVA TTDKEFSFKL GGRLQADYGR FDGYYTNNGN TADAAYFRRA YLEFGGTAYR DWKYQINYDL SRNVGNDSAG YFDEASVTYT GFNPVNLKFG RFYTDFGLEK ATSSKWVTAL ERNLTYDIAD WVNDNVGTGI QASSVVGGMA FLSGSVFSEN NNDTDGDSVK RYNLRGVFAP LHEPGNVVHL GLQYAYRDLE DSAVDTRIRP RMGMRGVSTN GGNDAGSNGN RGLFGGSSAV EGLWKDDSVW GLEGAWALGA FSAQAEYLRR TVKAERDRED LKASGYYAQL AYTLTGEPRL YKLDGAKFDT IKPENKEIGA WELFYRYDSI KVEDDNIVVD SATREVGDAK GKTHTLGVNW YANEAVKVSA NYVKAKTDKI SNANGDDSGD GLVMRLQYVF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Anion specific, the binding site has higher affinity for phosphate than chloride ions. Porin O has a higher affinity for polyphosphates (tripolyphosphate and pyrophosphate) while porin P has a higher affinity for orthophosphate.
  • 基因功能参考文献：
    1. Our analyzes show that OprO, and to a lesser degree OprP, have unexpected very high permeability to fosfomycin, so the antibiotic should be a potentially excellent alternative choice for the control of Pseudomonas aeruginosa infections. PMID: 29198700
    2. The results suggest that trimerization is crucial for both structure and function of general porin OmpF in E. coli, whereas being trimer in substrate-specific channel OprP supports a pore function in Pseudomonas aeruginosa. PMID: 26895142
    3. Study used x-ray crystallography, free-energy molecular dynamics (MD) simulations, and electrophysiology to uncover the atomic basis for the different substrate specificity of OprP and OprO PMID: 26445443
    4. Data indicate the role of the R133 residue - particularly its charge and ability to change the solvation behavior of the permeating ion - in the structure-function relationship of outer membrane porin OprP. PMID: 23875754
  • 亚细胞定位：
    Cell outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    OprO/OprP family
  • 数据库链接：

    KEGG: pae:PA3279

    STRING: 208964.PA3279