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  4. Recombinant Pseudomonas aeruginosa Porin D (oprD)

Recombinant Pseudomonas aeruginosa Porin D (oprD)

  • 货号：
    CSB-YP333726EZX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP333726EZX
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP333726EZX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP333726EZX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP333726EZX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    oprD
  • Uniprot No.：
  • 别名：
    oprD; PA0958; Porin D; EC 3.4.21.-; Imipenem/basic amino acid-specific outer membrane pore; Outer membrane protein D2
  • 种属：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    24-443
  • 氨基酸序列
    DAFVSDQ AEAKGFIEDS SLDLLLRNYY FNRDGKSGSG DRVDWTQGFL TTYESGFTQG TVGFGVDAFG YLGLKLDGTS DKTGTGNLPV MNDGKPRDDY SRAGGAVKVR ISKTMLKWGE MQPTAPVFAA GGSRLFPQTA TGFQLQSSEF EGLDLEAGHF TEGKEPTTVK SRGELYATYA GETAKSADFI GGRYAITDNL SASLYGAELE DIYRQYYLNS NYTIPLASDQ SLGFDFNIYR TNDEGKAKAG DISNTTWSLA AAYTLDAHTF TLAYQKVHGD QPFDYIGFGR NGSGAGGDSI FLANSVQYSD FNGPGEKSWQ ARYDLNLASY GVPGLTFMVR YINGKDIDGT KMSDNNVGYK NYGYGEDGKH HETNLEAKYV VQSGPAKDLS FRIRQAWHRA NADQGEGDQN EFRLIVDYPL SIL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Porin with a specificity for basic amino acids. Also possesses serine protease activity.
  • 基因功能参考文献：
    1. The results showed that OprD downregulation and IMP-type metallo-beta-lactamases are the main carbapenem resistance mechanisms in carbapenem-resistant Pseudomonas aeruginosa isolates from northwest of Iran. PMID: 28654368
    2. OprD polymorphisms, particularly the F170L substitution and the specific shortening in loop 7, appear to determine the potential for P. aeruginosa to develop carbapenem resistance [OprD]. PMID: 28535274
    3. P. aeruginosa isolates obtained from CF patients significantly bound vitronectin. Porin D was defined as a novel P. aeruginosa vitronectin-receptor PMID: 26047937
    4. strains with over-expression of OprD gene showed high sensitivity to carbapenems family antibiotics PMID: 25644798
    5. it may be envisaged that sialic acids on OprD protein play important role toward the uptake of commonly used antibiotics in PA(+Sias). This might be one of the new mechanisms of PA for beta-lactam antibiotic uptake. PMID: 24643970
    6. OprD inactivation and overexpression of efflux systems is the main carbapenem resistance mechanism. PMID: 22633564
    7. OprD inactivating mutations in clinical isolates of P. aeruginosa are not restricted only to carbapenem-resistant isolates PMID: 22290967
    8. Imipenem resistant strains lack OprD protein PMID: 16476803

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  • 亚细胞定位：
    Cell outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Outer membrane porin (Opr) (TC 1.B.25) family
  • 数据库链接：

    KEGG: pae:PA0958

    STRING: 208964.PA0958