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  4. Recombinant Pseudomonas aeruginosa Nitronate monooxygenase (PA1024)

Recombinant Pseudomonas aeruginosa Nitronate monooxygenase (PA1024)

  • 中文名称：
    铜绿假单胞菌PA1024重组蛋白
  • 货号：
    CSB-YP881223EZX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    铜绿假单胞菌PA1024重组蛋白
  • 货号：
    CSB-EP881223EZX
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    铜绿假单胞菌PA1024重组蛋白
  • 货号：
    CSB-EP881223EZX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    铜绿假单胞菌PA1024重组蛋白
  • 货号：
    CSB-BP881223EZX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    铜绿假单胞菌PA1024重组蛋白
  • 货号：
    CSB-MP881223EZX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PA1024
  • Uniprot No.：
  • 别名：
    PA1024; NADH:quinone reductase; EC 1.6.5.11
  • 种属：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-328
  • 氨基酸序列
    MGVFRTRFTE TFGVEHPIMQ GGMQWVGRAE MAAAVANAGG LATLSALTQP SPEALAAEIA RCRELTDRPF GVNLTLLPTQ KPVPYAEYRA AIIEAGIRVV ETAGNDPGEH IAEFRRHGVK VIHKCTAVRH ALKAERLGVD AVSIDGFECA GHPGEDDIPG LVLLPAAANR LRVPIIASGG FADGRGLVAA LALGADAINM GTRFLATREC PIHPAVKAAI RAADERSTDL IMRSLRNTAR VARNAISQEV LAIEARGGAG YADIAALVSG QRGRQVYQQG DTDLGIWSAG MVQGLIDDEP ACAELLRDIV EQARQLVRQR LEGMLAGV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the NADH-dependent reduction of a broad spectrum of quinone substrates, generating the corresponding hydroquinones. Highly prefers NADH to NADPH as a reducing substrate. Also displays a small NADH oxidase activity. Does not exhibit nitronate monooxygenase activity; is inactive against propionate 3-nitronate, 3-nitropropionate, nitroethane, 1-nitropropane, 2-nitropropane, and the anionic forms ethylnitronate, propyl-1-nitronate, and propyl-2-nitronate. Has no azoreductase activity since it is not able to reduce the azo dye methyl red with NADH. May be required to maintain an appropriate [NAD(+)]/[NADH] ratio for the catabolism of fatty acids in P.aeruginosa PAO1.
  • 基因功能参考文献：
    1. Purified PA1024 did not exhibit nitronate monooxygenase activity; however, it displayed NADH:quinone reductase and a small NADH:oxidase activity. The enzyme preferred NADH to NADPH as a reducing substrate. PMID: 27502282
  • 蛋白家族：
    Nitronate monooxygenase family
  • 数据库链接：

    KEGG: pae:PA1024

    STRING: 208964.PA1024