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  4. Recombinant Programmed cell death activator egl-1 (egl-1)

Recombinant Programmed cell death activator egl-1 (egl-1)

  • 中文名称：
    秀丽隐杆线虫egl-1重组蛋白
  • 货号：
    CSB-YP527671CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    秀丽隐杆线虫egl-1重组蛋白
  • 货号：
    CSB-EP527671CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    秀丽隐杆线虫egl-1重组蛋白
  • 货号：
    CSB-EP527671CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    秀丽隐杆线虫egl-1重组蛋白
  • 货号：
    CSB-BP527671CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    秀丽隐杆线虫egl-1重组蛋白
  • 货号：
    CSB-MP527671CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    egl-1
  • Uniprot No.：
  • 别名：
    egl-1; F23B12.9Programmed cell death activator egl-1; Egg-laying defective protein 1
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-106
  • 氨基酸序列
    MLMLTFASTS SDLLPMSNVF DVQSSVFYNE KNMFYSSSQD FSSCEDSSQF ADDSGFFDDS EISSIGYEIG SKLAAMCDDF DAQMMSYSAH ASDRSLFHRL LDFFAF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a major role in programmed cell death (PCD or apoptosis) by negatively regulating ced-9. Binds to and directly inhibits the activity of ced-9, releasing the cell death activator ced-4 from a ced-9/ced-4 containing protein complex and allowing ced-4 to activate the cell-killing caspase ced-3. Required to activate programmed cell death in the sister cells of the serotonergic neurosecretory motor (NSM) neurons during embryogenesis. Required to activate programmed cell death in the sister cells of the M4 motor neuron and I1 pharyngeal neuron during embryogenesis. During larval development, required for the elimination of transient presynaptic components upstream of ced-9, ced-4 and ced-3 apoptotic pathway. Together with ain-1, a component of the miRNA-induced-silencing complex (miRISC), and probably upstream of ced-3 and ced-4, regulates temporal cell fate patterning during larval development.
  • 基因功能参考文献：
    1. LIN-1 binds to, and activates transcription of, the key pro-apoptotic gene egl-1. Our results provide the first evidence that EGF induces PCD at the whole organism level and reveal the molecular basis for the death-promoting function of LIN-3/EGF PMID: 25144461
    2. The transcriptional upregulation of egl-1 in the CEMs in hermaphrodites is necessary, but not sufficient for their death. PMID: 20150917
    3. DNA damage increases expression of the proapoptotic gene egl-1, a response that requires hus-1 and the p53 homolog cep-1 PMID: 12445383
    4. the binding of EGL-1 to CED-9 is extremely stable PMID: 12894216
    5. Metabolism of mutants of the proapoptotic EGL-1 protein was studied in a functional analysis of the CED-1 protein. PMID: 15383288
    6. mitochondria fragment in cells that normally undergo programmed cell death during C. elegans development; fragmentation is induced by the BH3-only protein EGL-1 PMID: 15716954
    7. A complex between MAB-5 and the Pbx homolog CEH-20 directly regulates transcription of the BH3 domain gene egl-1 to initiate programmed cell death PMID: 16421192
    8. Consistent with its ability to neutralize CED-9 function, EGL-1 antagonized CED-9-dependent remodeling of the mitochondrial network. PMID: 16543146
    9. oxidative, osmotic, heat shock and starvation stresses induce germ cell apoptosis through a p53 and EGL-1 independent pathway. PMID: 16729024
    10. Data show that the worm protein EGL-1 binds mammalian pro-survival proteins very poorly, but can be converted into a high-affinity ligand for Bcl-2 and Bcl-x(L) by mutation of the cysteine residue at position 62 within the BH3 domain. PMID: 18566606
    11. Bcl-2 proteins EGL-1 and CED-9 do not regulate mitochondrial fission or fusion in Caenorhabditis elegans. PMID: 19327994
    12. EGL-1 triggers apoptosis induction predominantly by antagonizing the function of an anti-apoptotic member of the core BCL-2 family. Review. PMID: 19641505

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  • 亚细胞定位：
    Cell junction, synapse.
  • 数据库链接：

    KEGG: cel:CELE_F23B12.9

    STRING: 6239.F23B12.9

    UniGene: Cel.19641