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  4. Recombinant Probable ubiquitin conjugation factor E4 (ufd-2)

Recombinant Probable ubiquitin conjugation factor E4 (ufd-2)

  • 中文名称：
    秀丽隐杆线虫ufd-2重组蛋白
  • 货号：
    CSB-YP600619CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    秀丽隐杆线虫ufd-2重组蛋白
  • 货号：
    CSB-EP600619CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    秀丽隐杆线虫ufd-2重组蛋白
  • 货号：
    CSB-EP600619CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    秀丽隐杆线虫ufd-2重组蛋白
  • 货号：
    CSB-BP600619CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    秀丽隐杆线虫ufd-2重组蛋白
  • 货号：
    CSB-MP600619CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ufd-2
  • Uniprot No.：
  • 别名：
    ufd-2; T05H10.5; Ubiquitin conjugation factor E4 ufd-2; EC 2.3.2.27; E4 ubiquitin-protein ligase ufd-2; RING-type E3 ubiquitin transferase E4; Ubiquitin fusion degradation protein 2
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as an E4 ubiquitin ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. The elongation of preexisting ubiquitin chains preferentially targets ubiquitin 'Lys-29' and 'Lys-48' residues. Also functions as an E3 ligase in conjunction with specific E1 and E2 ligases. Probably by regulating protein ubiquitination at DNA damage repair sites, coordinates DNA double-strand-break repair and apoptosis in the germline. Required for germline apoptosis in response to DNA damage downstream of cep-1. Involved in the resolution of DNA-repair sites by promoting the release of rad-51 from DNA damage foci. In association with protein-ligase chn-1, acts as an E3/E4 ligase to poly-ubiquitinate lysine residues in the UCS domain of myosin chaperone unc-45. By targeting myosin chaperone unc-45 for proteasomal degradation, regulates myosin assembly in body wall muscles in association with cdc-48.1 and chn-1. However, in a contrasting study, acts as an E3 ligase, independently of chn-1, to poly-ubiquitinate unc-45 without promoting unc-45 proteasomal degradation. Instead, uses unc-45 as an adapter protein to recruit and poly-ubiquitinate unfolded myosin heavy chain B unc-54.
  • 基因功能参考文献：
    1. UFD-2 is not regulating the protein levels of UNC-45 in muscle cells, but rather shows the characteristic properties of a bona fide E3 ligase involved in protein quality control. UFD-2 preferentially targets unfolded protein segments. PMID: 29396393
    2. Ligase activity of UFD-2 triggers DNA double-strand breaks -induced apoptosis. UFD-2 supports RAD-51 dissociation from DNA-repair sites. PMID: 27669035
  • 亚细胞定位：
    Cytoplasm. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nucleolus.
  • 蛋白家族：
    Ubiquitin conjugation factor E4 family
  • 组织特异性：
    Expressed in the germline (at protein level).
  • 数据库链接：

    STRING: 6239.T05H10.5b

    UniGene: Cel.7502