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  4. Recombinant Pig Steroid 21-hydroxylase (CYP21)

Recombinant Pig Steroid 21-hydroxylase (CYP21)

  • 货号：
    CSB-YP325757PI
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP325757PI
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP325757PI-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP325757PI
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP325757PI
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CYP21
  • Uniprot No.：
  • 别名：
    CYP21; CYP21A1; CYP21A3Steroid 21-hydroxylase; EC 1.14.14.16; 21-OHase; Cytochrome P-450c21; Cytochrome P450 21; Cytochrome P450 XXI; Cytochrome P450-C21
  • 种属：
    Sus scrofa (Pig)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-492
  • 氨基酸序列
    MVLVWLLLLL TLLAGARLLW GQWKLRNLHL PPLVPGFLHL LQPNLPIYLL GLTQRLGPIY RLRLGLQDVV VLNSKRTIEE ALVRKWVDFA GRPQIPSYKL ASQHCPDISL GDYSLFWKAH KKLTRSALLL GVRSSMEPRV EQLTQEFCER MRAQAGTPVT IQKEFSVLTC SIICCLTFGD KEDTLVHALH DCVQDLMKTW EHWSIQILDM VPFLRFFPSP GLRRLKQAIE NRDHLVEKQL RRHKESMVAG QWRDMLDYML QEAGRQRVEE GQGQLLEGHV HMSVVDLFIG GTETTANTLS WAVVYLLHHP EIQWRLQEEL DRELGPGAAG SRVPYKDRAR LPLLNATIAE VLRLRPVVPL ALPHRATRPS SIFGYDIPEG TVVIPNLQGA HLDETVWEQP HEFRPDRFLA PGANPSALAF GCGARVCLGE PLARLELFVV LVQLLQAFTL LPPEGALPSL QPHPHSGINL KVQPFQVRLQ PRGGRGEGPG PR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
  • 基因功能参考文献：
    1. It plays a role on cortisol production and depends on stress. And expression of CYP21 protein will provide the basis for information and better understanding of the mechanisms related to drip loss in pork. PMID: 28677294
    2. CYP21 gene polymorphisms were analyzed in association with litter size. PMID: 16965408
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 数据库链接：

    KEGG: ssc:403337

    STRING: 9823.ENSSSCP00000001529

    UniGene: Ssc.52818