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  4. Recombinant Partitioning defective protein 6 (par-6)

Recombinant Partitioning defective protein 6 (par-6)

  • 中文名称：
    秀丽隐杆线虫par-6重组蛋白
  • 货号：
    CSB-YP885652CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    秀丽隐杆线虫par-6重组蛋白
  • 货号：
    CSB-EP885652CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    秀丽隐杆线虫par-6重组蛋白
  • 货号：
    CSB-EP885652CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    秀丽隐杆线虫par-6重组蛋白
  • 货号：
    CSB-BP885652CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    秀丽隐杆线虫par-6重组蛋白
  • 货号：
    CSB-MP885652CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    par-6
  • Uniprot No.：
  • 别名：
    par-6; T26E3.3Partitioning defective protein 6
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-309
  • 氨基酸序列
    MSYNGSYHQN HHSTLQVKSK FDSEWRRFSI PMHSASGVSY DGFRSLVEKL HHLESVQFTL CYNSTGGDLL PITNDDNLRK SFESARPLLR LLIQRRGESW EEKYGYGTDS DKRWKGISSL MAQKPPKRSY SISNPEDFRQ VSAIIDVDIV PEAHRRVRLC KHGQERPLGF YIRDGTSVRV TERGVVKVSG IFISRLVDGG LAESTGLLGV NDEVLEVNGI EVLGKTLDQV TDMMVANAHN LIITVKPANQ RNTLSRGPSQ QGTPNASEMS AATAAATGGI QRPMKMNGSS DGSYHPKQHD ANDSDSGED
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Necessary for apicobasal and anterior-posterior asymmetries associated with cell adhesion and gastrulation during the first few cell cycles of embryogenesis. Required for localizing/ maintaining par-3 at the cell periphery. Regulates mes-1 expression and/or localization pattern during early embryogenesis. Acts together with par-3 and pkc-3 in maintaining epithelial cell polarity in the distal spermatheca. Plays a role in endosome and Golgi body positioning.
  • 基因功能参考文献：
    1. we find that vab-1 lies in the same genetic pathway as cdc-42 and is responsible for polarizing CDC-42 activity to the medial tip. Together, these data establish a previously uncharacterized role for polarized CDC-42, in conjunction with PAR-6, PAR-3 and an Eph receptor(vab-1), during epithelial intercalation. PMID: 27861585
    2. the arcade cell epithelium polarizes by a PAR-6-mediated pathway that is independent of E-cadherin, beta-integrin and beta-laminin. PMID: 25773364
    3. PAR-3 and PAR-6 function in cytokinesis may be partially redundant and play a role in the maintenance of DYN-1 in the cleavage furrow. PMID: 22887994
    4. evidence PAR polarity arises from coupling of advective transport by flowing cell cortex to a multistable PAR reaction-diffusion system; advection in active, flowing medium provides mechanism for mechanically templated pattern formation in development PMID: 22021673
    5. Centrosomes localize apically by first moving toward lateral foci of the conserved polarity proteins PAR-3 and PAR-6, then move together with these foci toward the future apical surface. Embryos lacking PAR-3 fail to localize their centrosomes apically. PMID: 22425160
    6. PAR-2 and PAR-6, which localize to opposing PAR domains, undergo exchange between well mixed cytoplasmic populations and laterally diffusing membrane-associated states PMID: 21518794
    7. Binding to PKC-3, but not to PAR-3 or to a conventional PDZ domain ligand, is required for PAR-6 function in C. elegans PMID: 20122916
    8. PAR proteins function in both apicobasal and anterior-posterior asymmetry during the first few cell cycles of embryogenesis. PMID: 13129846
    9. CDC-37-mediated inhibition of the CDC-42-dependent binding site and PAR-3-mediated release of this inhibition provide a key mechanism for the anterior accumulation of PAR-6. PMID: 16943281
    10. CDC-42 interaction with PAR-6 is not required for the initial establishment of asymmetry but is required for maximal cortical accumulation of PAR-6 and to maintain its asymmetry. PMID: 16996049
    11. PAR-6 is required for junction formation but not apicobasal polarization in C. elegans embryonic epithelial cells. PMID: 17314130
    12. nos-3 loss of function mutant suppresses the lethality of par-2 mutants by regulating PAR-6 protein levels. PMID: 18502413
    13. study identified a RhoGAP, PAC-1, which mediates embryo radial polarity & gastrulation by excluding PAR-6 from contacted cell surfaces; PAC-1 provides a dynamic molecular link between cell contacts & PAR proteins that polarizes embryos radially PMID: 18583611

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  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein. Cell junction, tight junction.
  • 蛋白家族：
    PAR6 family
  • 组织特异性：
    Colocalized with par-3 at all stages in early embryos, at the anterior cortex of the embryo. Patchy expression observed at the periphery after completion of meiosis I and in meiosis II, which on completion of metaphase II, is restricted to the anterior 85
  • 数据库链接：

    KEGG: cel:CELE_T26E3.3

    STRING: 6239.T26E3.3a.3

    UniGene: Cel.8562