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  4. Recombinant Pariacoto virus Capsid protein alpha (alpha)

Recombinant Pariacoto virus Capsid protein alpha (alpha)

  • 中文名称：
    Recombinant Pariacoto virus Capsid protein alpha(alpha),Yeast
  • 货号：
    CSB-YP864300PMAN
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Pariacoto virus Capsid protein alpha(alpha),Yeast
  • 货号：
    CSB-EP864300PMAN
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Pariacoto virus Capsid protein alpha(alpha),Yeast
  • 货号：
    CSB-EP864300PMAN-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Pariacoto virus Capsid protein alpha(alpha),Yeast
  • 货号：
    CSB-BP864300PMAN
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Pariacoto virus Capsid protein alpha(alpha),Yeast
  • 货号：
    CSB-MP864300PMAN
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    alpha
  • Uniprot No.：
  • 别名：
    alpha; Capsid protein alpha [Cleaved into: Capsid protein beta; EC 3.4.23.44; Coat protein beta; Nodavirus endopeptidase); Peptide gamma; Coat protein gamma)]
  • 种属：
    Pariacoto virus (PaV)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-401
  • 氨基酸序列
    MVSRTKNRRN KARKVVSRST ALVPMAPASQ RTGPAPRKPR KRNQALVRNP RLTDAGLAFL KCAFAAPDFS VDPGKGIPDN FHGRTLAIKD CNTTSVVFTP NTDTYIVVAP VPGFAYFRAE VAVGAQPTTF VGVPYPTYAT NFGAGSQNGL PAVNNYSKFR YASMACGLYP TSNMMQFSGS VQVWRVDLNL SEAVNPAVTA ITPAPGVFAN FVDKRINGLR GIRPLAPRDN YSGNFIDGAY TFAFDKSTDF EWCDFVRSLE FSESNVLGAA TAMKLLAPGG GTDTTLTGLG NVNTLVYKIS TPTGAVNTAI LRTWNCIELQ PYTDSALFQF SGVSPPFDPL ALECYHNLKM RFPVAVSSRE NSKFWEGVLR VLNQISGTLS VIPGPVGTIS AGVHQLTGMY M
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Capsid protein alpha self-assembles to form an icosahedral procapsid with a T=3 symmetry, about 30 nm in diameter, and consisting of 60 capsid proteins trimers. In addition, calcium ions are incorporated per capsid during assembly. The capsid encapsulates the two genomic RNAs. Capsid maturation occurs via autoproteolytic cleavage of capsid protein alpha generating capsid protein beta and the membrane-active peptide gamma.; membrane-permeabilizing peptide produced by virus maturation, thereby creating the infectious virion. After endocytosis into the host cell, peptide gamma is probably exposed in endosomes, where it permeabilizes the endosomal membrane, facilitating translocation of viral capsid or RNA into the cytoplasm.
  • 基因功能参考文献：
    1. A new concept in virus biology demonstrates the PaV capsid formed from 180 identical coat proteins clustered as 60 trimers, part of a wider set of structural constraints on virus structure. PMID: 23403965
  • 亚细胞定位：
    [Capsid protein beta]: Virion.; [Peptide gamma]: Virion.
  • 蛋白家族：
    Peptidase A6 family
  • 数据库链接：

    KEGG: vg:956349