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  4. Recombinant Mycobacterium tuberculosis Diacylglycerol acyltransferase/mycolyltransferase Ag85B (fbpB)

Recombinant Mycobacterium tuberculosis Diacylglycerol acyltransferase/mycolyltransferase Ag85B (fbpB)

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  • 中文名称：
    结核分枝杆菌fbpB重组蛋白
  • 货号：
    CSB-YP314366MVZ
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP314366MVZ could indicate that this peptide derived from Yeast-expressed Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) fbpB.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP314366MVZ could indicate that this peptide derived from Yeast-expressed Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) fbpB.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    fbpB
  • Uniprot No.：
  • 别名：
    fbpB; MT1934Diacylglycerol acyltransferase/mycolyltransferase Ag85B; DGAT; EC 2.3.1.122; EC 2.3.1.20; 30 kDa extracellular protein; Acyl-CoA:diacylglycerol acyltransferase; Antigen 85 complex B; 85B; Ag85B; Extracellular alpha-antigen; Fibronectin-binding protein B; Fbps B
  • 种属：
    Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    32.7kDa
  • 表达区域：
    41-325aa
  • 氨基酸序列
    FSRPGLPVEYLQVPSPSMGRDIKVQFQSGGNNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETFLTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGDAGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKFQDAYNAAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGAG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    CUSABIO used yeast cells to express N-terminal 6xHis-tagged Mycobacterium tuberculosis Diacylglycerol acyltransferase/mycolyltransferase Ag85B (fbpB). This recombinant fbpB protein is full-length of mature protein containing 41-325aa of mycobacterium tuberculosis fbpB. It underwent validation via LC-MS/MS analysis. Its purity reached up to 90% determined by SDS-PAGE. Under reducing conditions, a molecular weight band of around 33 kDa was visualized on the SDS-PAGE.

    Ag85B, the fbpB encoding protein, is a component of the Ag85 complex that synthesizes the majority of Mycobacterium tuberculosis-secreted proteins. The proteins containing the Ag85 complex play a crucial role in the final step of cell wall assembly and the maintenance of the bacterial cell envelope integrity. This effect is carried out by catalysis of the biosynthesis of abundant cell envelope components, including TMM and TDM. Also, the Ag85 complex is involved in the synthesis of trehalose dimycolate. The Mycobacterium tuberculosis Ag85 complex has been demonstrated to stimulate a strong humoral- and cell-mediated immune response.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Mycobacterial A85 antigen family
  • 数据库链接：

    KEGG: mtc:MT1934