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  4. Recombinant Mouse cAMP-dependent protein kinase type II-beta regulatory subunit (Prkar2b)

Recombinant Mouse cAMP-dependent protein kinase type II-beta regulatory subunit (Prkar2b)

  • 中文名称：
    小鼠Prkar2b重组蛋白
  • 货号：
    CSB-YP018698MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Prkar2b重组蛋白
  • 货号：
    CSB-EP018698MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Prkar2b重组蛋白
  • 货号：
    CSB-EP018698MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Prkar2b重组蛋白
  • 货号：
    CSB-BP018698MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Prkar2b重组蛋白
  • 货号：
    CSB-MP018698MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Prkar2b; cAMP-dependent protein kinase type II-beta regulatory subunit
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-416
  • 氨基酸序列
    MSIEIPAGLT ELLQGFTVEV LRHQPADLLE FALQHFTRLQ QENERKGAAR FGHEGRTWGD AGAAAGGGIP SKGVNFAEEP MRSDSENGEE EEAAEAGAFN APVINRFTRR ASVCAEAYNP DEEEDDAESR IIHPKTDDQR NRLQEACKDI LLFKNLDPEQ MSQVLDAMFE KLVKEGEHVI DQGDDGDNFY VIDRGTFDIY VKCDGVGRCV GNYDNRGSFG ELALMYNTPR AATITATSPG ALWGLDRVTF RRIIVKNNAK KRKMYESFIE SLPFLKSLEV SERLKVVDVI GTKVYNDGEQ IIAQGDLADS FFIVESGEVK ITMKRKGKSE VEENGAVEIA RCFRGQYFGE LALVTNKPRA ASAHAIGTVK CLAMDVQAFE RLLGPCMEIM KRNIATYEEQ LVALFGTNMD IVEPTA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
  • 基因功能参考文献：
    1. RNAi-mediated Prkar2b suppression resulted in MI-stage arrest during oocyte development, and these oocytes exhibited abnormal spindle formation and chromosome aggregation. PMID: 29518769
    2. deficiency induces brownlike adipocytes in inguinal white adipose tissue, increases expression of uncoupling protein1 and other thermogenic genes PMID: 27967236
    3. Data (including data from studies using knockout mice) suggest that S100A1 (S-100 calcium-binding protein A1, alpha chain) is involved in protein kinase A- (RIIalpha and RIIbeta)-dependent signaling resulting in nuclear redistribution/influx of HDAC4 (histone deacetylase 4) in skeletal muscle fibers. PMID: 28409622
    4. RIIbeta-PKA modulates the duration of leptin receptor signalling and therefore the magnitude of the catabolic response to leptin. PMID: 26381935
    5. Release of pRIIbeta in the presence of cAMP is reduced by calcium, whereas autophosphorylation at the phosphorylation site inhibits holoenzyme reassociation with the catalytic subunit. PMID: 26158466
    6. RIIBeta protein kinase A knockout was protective against induced seizure susceptibility. PMID: 24104609
    7. Deficiency of the RIIbeta subunit of PKA affects locomotor activity and energy homeostasis in distinct neuronal populations. PMID: 23569242
    8. study describes the 2.3 angstrom structure of full-length tetrameric RIIbeta(2):C(2) holoenzyme PMID: 22323819
    9. Conditioned taste aversion (CTA) learning was examined in mice with a targeted disruption of a gene for a specific regulatory subunit of PKA (RIIbeta), which is selectively expressed in amygdala. Disruption of PKA signaling interferes with CTA. PMID: 12842296
    10. RII-PKAII complements TSHR action by stably propagating robust cAMP signals in cell compartments PMID: 12902333
    11. Here we show that visual cortical plasticity remains intact in AC1/AC8-/- mice, whereas Ocular dominance plasticity and LTD, but not LTP, are absent in RIIbeta-/- mice PMID: 15483123
    12. Disruption of the RIIbeta regulatory subunit of protein kinase A (PKA) results in mice with a lean phenotype, nocturnal hyperactivity, and increased resting metabolic rate. PMID: 15618289
    13. An activity-dependent model for barrel map development in brain circuitry is revealed that is mediated by a cAMP/PKA-dependent pathway that relies on PKARIIbeta function. PMID: 16624954
    14. increased ingestion of ethanol by protein kinase A(PKA) subunit RIIbeta(-/-) mice is likely the result of altered PKA activity within neuronal pathways that control ethanol-consummatory behaviors PMID: 16634851

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  • 亚细胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    CAMP-dependent kinase regulatory chain family
  • 组织特异性：
    Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • 数据库链接：

    KEGG: mmu:19088

    STRING: 10090.ENSMUSP00000003079

    UniGene: Mm.25594