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  4. Recombinant Mouse Zinc finger protein SNAI2 (Snai2)

Recombinant Mouse Zinc finger protein SNAI2 (Snai2)

  • 货号：
    CSB-YP021869MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021869MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021869MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021869MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021869MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Snai2; Slug; Slugh; Zinc finger protein SNAI2; Neural crest transcription factor Slug; Protein snail homolog 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-269
  • 氨基酸序列
    MPRSFLVKKH FNASKKPNYS ELDTHTVIIS PYLYESYPIP VIPKPEILTS GAYSPITVWT SSAAPLHSPL PSGLSPLTGY SSSLGRVSPP PSSDTSSKDH SGSESPISDE EERLQPKLSD PHAIEAEKFQ CNLCNKTYST FSGLAKHKQL HCDAQSRKSF SCKYCDKEYV SLGALKMHIR THTLPCVCKI CGKAFSRPWL LQGHIRTHTG EKPFSCPHCN RAFADRSNLR AHLQTHSDVK KYQCKNCSKT FSRMSLLHKH EESGCCVAH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.
  • 基因功能参考文献：
    1. Increased SNAI2 expression is associated with the development of severe pulmonary hypertension. PMID: 29074488
    2. transgenic mice expressing Slug and Kras in acinar cells were generated. Surprisingly, Slug attenuated Kras-induced acinar-ductal metaplasia (ADM)development, ERK1/2 phosphorylation and proliferation. Co-expression of Slug with Kras also attenuated chronic pancreatitis-induced changes in ADM development and fibrosis PMID: 27364947
    3. Results indicate that vimentin orchestrates the healing by controlling fibroblast proliferation, TGF-beta1-Slug signaling, and epithelial-mesenchymal transition (EMT) processing, and all of which in turn govern the required keratinocyte activation. PMID: 27466403
    4. our current findings highlight how Slug functions as an important transcriptional repressor that finely regulates the SCF/c-Kit signaling pathway. PMID: 27451973
    5. Slug emerges as a key transcription factor driving smooth muscle cell towards a proliferative phenotype. PMID: 27441378
    6. both Snail and Slug are able to form binary complexes with either YAP or TAZ that, together, control YAP/TAZ transcriptional activity and function throughout mouse development. PMID: 28112996
    7. results demonstrate that skeletal stem/stromal cell mobilize Snail/Slug-YAP/TAZ complexes to control stem cell function PMID: 27479603
    8. The data presented here demonstrate that Snai2 and Snai3 transcriptionally regulate the cellular fitness and functionality of not only CD4(+) regulatory T cells but effector CD8(alpha+) and CD4(+) conventional T cells as well. PMID: 26831822
    9. TGFbeta3 increases IRF6 expression and subsequently regulates SNAI2 expression; IRF6 appears to regulate epithelial mesenchymal transition during palatal fusion via SNAI2. PMID: 26240017
    10. Our work demonstrates the participation of SNAI2 in the pathogenesis of luminal breast cancer, and reveals an unexpected connection between the processes of postlactational involution and breast tumorigenesis in Snai2-null mutant mice. PMID: 26096931
    11. SNAI2-driven BIM-induced apoptosis may temper metastasis by governing the survival of disseminating breast tumor cells. PMID: 25263453
    12. Data indicate that deletion of snail family transcription factors Snai2 and Snai3 in bone marrow-derived cells is sufficient to induce autoantibody generation. PMID: 25732600
    13. Loss of Snail2 favors skin tumor progression by promoting the recruitment of myeloid progenitors. PMID: 25784375
    14. p19(Arf) (p14(ARF) in human) stabilizes Slug to inhibit E-cadherin in prostate cancer mouse models. PMID: 24910389
    15. SNAIL and SLUG probably play important roles during follicular development, luteinization and early embryonic development. PMID: 25092544
    16. Nanog functions in conjunction with mesenchymal factors Snai1 and Snai2 in the transcriptional regulation of pluripotency-associated genes and miRNAs during the Nanog-driven reprogramming process. PMID: 25240402
    17. pregnancy-associated mammary stem cells require a TGF-beta2/alphavbeta3/Slug pathway PMID: 25117682
    18. Slug promotes survival during metastasis through suppression of Puma-mediated apoptosis. PMID: 24830722
    19. Snai2 is downregulated by glyoxal in a process that causes defective keratinocyte migration PMID: 24210586
    20. The actin-bundling protein fascin is regulated by slug and involved in late-stage pancreatic intraepithelial neoplasms and pancreatic ductal adenocarcinoma formation in mice. PMID: 24462734
    21. Data show that Snail1 efficient repression and binding to E-cadherin promoter as well as epithelial-to-mesenchymal transition (EMT)-inducing ability require intact ZF1 and ZF2, while for Snail2, either ZF3 or ZF4 is essential for those functions. PMID: 24297167
    22. Our results failed to demonstrate a relationship between Slug and P53 expression in skin cancer. PMID: 24008423
    23. Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency. PMID: 23874916
    24. the Snai1 and Snai2 genes transcriptionally compensate temporally, spatially, and quantitatively for each other's loss, and demonstrate an essential role for Snail family genes during chondrogenesis in mice. PMID: 23322385
    25. Data indicate that Slug and Snail are sufficient for the induction of single-cell invasion in an in vitro invasion assay and in an embryonic zebrafish xenograft model. PMID: 23618854
    26. Tbx18 and Wt1 directly bound to the Slug promoter region and regulated Slug expression PMID: 23469079
    27. Expression of the Snai1 and Snai2 genes is negatively regulated by their protein products occupying each other's promoter during chondrogenesis, and provides an explanation for genetic redundancy observed in loss of function models PMID: 23665016
    28. We conclude that Slug pathway controls the growth dynamics of a subpopulation of cycling progenitor basal cells during mammary morphogenesis. PMID: 23300933
    29. Kruppel-like factor 4, a tumor suppressor in hepatocellular carcinoma cells reverts epithelial mesenchymal transition by suppressing slug expression. PMID: 22937066
    30. There is a reciprocal regulation between Slug and AR not only in transcriptional regulation but also in protein bioactivity, and Slug-AR complex plays an important role in accelerating the androgen-independent outgrowth of CRPC. PMID: 22745193
    31. Elf5 inhibits the epithelial-mesenchymal transition in mammary gland development and breast cancer metastasis by transcriptionally repressing Snail2. PMID: 23086238
    32. In primary myoblasts, snai1-HDAC1/2 repressive complex binds and excludes MyoD from its targets. PMID: 22771117
    33. Study identified two transcription factors, Slug and Sox9, that act cooperatively to determine the mammary stem cell (MaSC) state. Inhibition of either Slug or Sox9 blocks MaSC activity in primary mammary epithelial cells. PMID: 22385965
    34. SLUG is the dominant regulator of epithelial-mesenchymal transition in prostate cancer PMID: 22203039
    35. Slug is regulated during the process of corneal wound healing in the corneal epithelium in vivo, providing a novel insight into the EMT and Slug expression in corneal wound healing. PMID: 22247468
    36. Slug mediates Epithelial-to-mesenchymal transition (EMT) with enhanced in vivo rectal tumor formation. PMID: 21470622
    37. Snail1, Snail2, and E47 can promote collective migration during branching morphogenesis of mammary epithelial tissues through key regulators of epithelial-mesenchymal transition . PMID: 21610693
    38. Dioxin receptor and SLUG transcription factors regulate the insulator activity of B1 SINE retrotransposons via an RNA polymerase switch. PMID: 21324874
    39. results show that Twist1 needs to induce Snail2 to suppress the epithelial branch of the EMT program and that Twist1 and Snail2 act together to promote EMT and tumor metastasis PMID: 21199805
    40. Slug deficiency does not disturb hematopoiesis or alter HSC homeostasis and differentiation in bone marrow but increases the numbers of primitive hematopoietic cells in the extramedullary spleen site. PMID: 20032500
    41. This study implicates SNAI1 and SNAI2 in the lineage segregation of the trophectoderm and inner cell mass, and provides new insight into these oncogenes. PMID: 20046880
    42. novel downstream target of MyoD PMID: 12023284
    43. SLUG may be the main effector of microphthalmia (MITF) gene action in melanoblasts. PMID: 12444107
    44. The Slug gene is highly expressed in the mesenchymal or stromal component of numerous fetal organs. PMID: 12552634
    45. Slug is the molecular target that mediates the radioprotection through SCF/c-kit and be a molecular component conferring radioresistance to cancer cells PMID: 12833143
    46. Data suggest that Slug plays an important role in wound re-epithelialization in adult skin, and that Slug controls some aspects of epithelial cell behavior in adult tissues as well as during embryonic development. PMID: 15389643
    47. Slug has a role in the pathogenesis of mesenchymal tumours PMID: 15735690
    48. Slug functions downstream of p53 in developing blood cells as a critical switch that prevents their apoptosis by antagonizing the trans-activation of puma by p53. PMID: 16286009
    49. although some aspects of Snail family gene function, such as a role in left-right asymmetry determination, appear to be evolutionarily conserved, their role in neural crest cell formation and delamination is not PMID: 16801545
    50. 228 genes & 15 ESTs were differentially expressed in MDCK-derived lines transfected with Snail, Slug or E47 vs controls. These 3 transcription factors induce common & specific genetic programs showing a differential role in tumor progression & invasion. PMID: 17018611

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Snail C2H2-type zinc-finger protein family
  • 数据库链接：

    KEGG: mmu:20583

    STRING: 10090.ENSMUSP00000023356

    UniGene: Mm.4272