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  4. Recombinant Mouse WW domain-containing transcription regulator protein 1 (Wwtr1)

Recombinant Mouse WW domain-containing transcription regulator protein 1 (Wwtr1)

  • 中文名称：
    小鼠Wwtr1重组蛋白
  • 货号：
    CSB-YP887643MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Wwtr1重组蛋白
  • 货号：
    CSB-EP887643MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Wwtr1重组蛋白
  • 货号：
    CSB-EP887643MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Wwtr1重组蛋白
  • 货号：
    CSB-BP887643MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Wwtr1重组蛋白
  • 货号：
    CSB-MP887643MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Wwtr1; Taz; WW domain-containing transcription regulator protein 1; Transcriptional coactivator with PDZ-binding motif
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-395
  • 氨基酸序列
    MNPSSVPHPL PPPGQQVIHV TQDLDTDLEA LFNSVMNPKP SSWRKKILPE SFFKEPDSGS HSRQSSTDSS GGHPGPRLAG GAQHVRSHSS PASLQLGTGA GAAGGPAQQH AHLRQQSYDV TDELPLPPGW EMTFTATGQR YFLNHIEKIT TWQDPRKVMN QPLNHVNLHP SITSTSVPQR SMAVSQPNLA MNHQHQQVVA TSLSPQNHPT QNQPTGLMSV PNALTTQQQQ QQKLRLQRIQ MERERIRMRQ EELMRQEAAL CRQLPMETET MAPVNTPAMS TDMRSVTNSS SDPFLNGGPY HSREQSTDSG LGLGCYSVPT TPEDFLSNMD EMDTGENSGQ TPMTVNPQQT RFPDFLDCLP GTNVDLGTLE SEDLIPLFND VESALNKSEP FLTWL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
  • 基因功能参考文献：
    1. YAP/TAZ mechanotransduction integrates with cell-cell communication pathways for fine-grained orchestration of stem cell decisions. PMID: 28513598
    2. MOB1-dependent YAP1/TAZ-TEAD complex functions as a transcriptional repressor of SOX9 and thereby negatively regulates chondrogenesis. PMID: 29511023
    3. TAZ regulates cell fate depends on not only the cell type but also its subcellular localization. PMID: 28844657
    4. The results suggested that TAZ may suppress apoptosis and premature senescence in spermatogenic cells by inhibiting the p53-p21 signaling pathway, thus playing important roles in the maintenance and control of reproductive function. PMID: 28613007
    5. The role of Yap and Wwtr1 in the Hippo signaling pathway and the regulation of spermatogenesis in mice are reported. PMID: 28637242
    6. Taz and Yap have overlapping functions in promoting myoblast proliferation but Taz then switches to enhance myogenic differentiation. PMID: 28589555
    7. A crucial role for YAP and TAZ in the maintenance of the postnatal adrenal cortex. PMID: 28938438
    8. TAZ is a critical factor for SRC kinase-mediated intestinal tumor formation and regeneration. PMID: 28939028
    9. YAP/TAZ plays multifaceted roles for endothelial cell behaviors, proliferation, junction assembly, and metabolism in sprouting angiogenesis and barrier formation PMID: 28805663
    10. Yap and Taz leads to impaired epicardial epithelial-to-mesenchymal transition (EMT) and a reduction in epicardial cell proliferation and differentiation into coronary endothelial cells. PMID: 27160901
    11. This study identifies YAP/TAZ as central mediators of VEGF signaling PMID: 28867486
    12. TAZ is required for TGFbeta induction of smooth muscle genes and is also required for the differentiated VSMC phenotype; synergy between TAZ and SRF, and TAZ and Myocardin (MyoC856), in regulating smooth muscle gene activation was observed in primary aortic vascular smooth muscle cells. PMID: 28342289
    13. Results demonstrate a pivotal role for TAZ in regulating the differentiation of Treg cells and TH17 cells. PMID: 28504697
    14. ABL potentiated the assembly and activation of the RUNX2-TAZ master transcription factor complex that is required for osteoblastogenesis, while antagonizing PPARgamma-mediated adipogenesis. PMID: 27797343
    15. the transcription regulators YAP and TAZ localise to the nucleus in the basal layer of skin and are elevated upon wound healing. PMID: 26989177
    16. These results suggest that vinculin promotes the nuclear localization of transcription factor TAZ to inhibit the adipocyte differentiation on rigid extracellular matrix. PMID: 28115535
    17. TAZ represents a previously unrecognized factor that contributes to the critical process of steatosis-to-Nonalcoholic Steatohepatitis progression. PMID: 28068223
    18. both Snail and Slug are able to form binary complexes with either YAP or TAZ that, together, control YAP/TAZ transcriptional activity and function throughout mouse development. PMID: 28112996
    19. results demonstrate that skeletal stem/stromal cell mobilize Snail/Slug-YAP/TAZ complexes to control stem cell function PMID: 27479603
    20. Expression of TAZ promotes embryonic neural stem cell maintenance by enhancing stemness of neural stem cells during mammalian brain development. PMID: 25634692
    21. Tead2 transcription factors are crucial regulators of the cellular distribution of Yap and Taz, and together they control the expression of genes critical for EMT and metastasis. PMID: 24554433
    22. findings uncover important roles for Yap and Taz in cranial neural crest diversification and development PMID: 26718006
    23. Study discovered that YAP/TAZ are bona fide downstream effectors of the alternative Wnt signaling pathway. PMID: 26276632
    24. Hippo signalling effectors, YAP and TAZ, promote both the proliferation of intestinal stem/progenitor cells and their differentiation into goblet cells. PMID: 25531778
    25. This study identifies a novel mechanism of TAZ regulation by YAP, which has significant implications for our understanding of Hippo pathway regulation, YAP-isoform specific signaling, and the role of these proteins in cell proliferation, apoptosis, and tumorigenesis PMID: 26432639
    26. Disrupting Yap/Taz activities enhances Ret pathway activity and contributes to pathogenesis of lower urinary tract defects PMID: 26243870
    27. Immortalized fibroblasts conditionally expressing active YAP or TAZ mutant proteins overcome soft matrix limitations on growth and promote fibrosis PMID: 25502501
    28. YAP/TAZ regulate transcriptional elongation from the enhancer elements by recruiting the Mediator complex and promoting phenotypes of overgrowth and tumorigenesis PMID: 26439301
    29. A YAP/TAZ-miR-130/301 molecular circuit exerts systems-level control of fibrosis in a network of diseases and physiologic conditions PMID: 26667495
    30. Data show that low-power laser irradiation (LPLI) has an osteogenic potential by promoting osteoblast differentiation via TAZ (transcriptional co-activator with PDZ-binding motif) activation dependent on Akt/GSK3beta signaling pathway. PMID: 26159930
    31. These results suggest that YAP and TAZ localization to the nucleus is required for skin wound healing. PMID: 24108406
    32. FGF2 induced TAZ expression was stimulated by ERK (extracellular signal-regulated kinase) activation. PMID: 24125755
    33. Knockdown of the Hippo mediators Yap1 or Taz decreased in vitro cellular migration and transplantation of metastatic disease. PMID: 24497554
    34. ECG stimulates osteoblast differentiation through the activation of TAZ and RUNX2, revealing a novel mechanism for green tea-stimulated osteoblast differentiation PMID: 24515112
    35. Report comprehensive interactomes for TAZ. PMID: 24573087
    36. The Hippo signaling pathway and the effectors TAZ and YAP regulated the differentiation of mesenchymal stem cells. [Review] PMID: 24579311
    37. SelW enhances myoblast differentiation by inhibiting TAZ binding to 14-3-3. PMID: 24726955
    38. Study shows that in the absence of Wnt, YAP and TAZ are components of the destruction complex to which YAP/TAZ associate by binding to Axin1; s propose that the destruction complex serves as cytoplasmic anchor and functional sink for YAP/TAZ. PMID: 24976009
    39. TAZ interacts with components of the SWI/SNF complex to modulate lineage-specific gene expression. PMID: 24613358
    40. TAZ activation is a general feature of Wnt signaling and is functionally relevant to mediate Wnt biological effects. PMID: 23245942
    41. TM-25659 enhanced nuclear TAZ localization. PMID: 21913895
    42. findings identify YAP/TAZ as sensors and mediators of mechanical cues instructed by the cellular microenvironment PMID: 21654799
    43. These findings suggest that the expression of TAZ protein is involved in osteoblast proliferation and differentiation. PMID: 18067853
    44. TAZ is a coactivator for Pax8 and TTF-1 and plays a role in the control of genes involved in thyroid development and differentiation. PMID: 19010321
    45. The results indicate the importance of TAZ in lung alveolarization and its involvement in the pathogenesis of lung fibrosis. PMID: 19498055
    46. Functional interaction between paired box gene 3 (Pax3) and TAZ may provide a clue to clarifying the mechanism by which Pax3 serves as a transcriptional activator during embryogenesis PMID: 16300735
    47. Taz promotes PC2 degradation through a SCFbeta-Trcp E3 ligase complex. PMID: 17636028
    48. TAZ is a transcription coactivator for Cbfa1 and may be involved in the regulation of osteoblast differentiation. PMID: 12529404
    49. TAZ interacts with TTF-1 and regulates expression of surfactant protein-C PMID: 14970209
    50. the involvement of NHERF1 and TAZ in mediating the effects of the ephrin B1 ligand on osteoblast function was studied. PMID: 19995908

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cell membrane.
  • 组织特异性：
    Highly expressed in kidney, heart, placenta and lung.
  • 数据库链接：

    KEGG: mmu:97064

    STRING: 10090.ENSMUSP00000113040

    UniGene: Mm.405029