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  4. Recombinant Mouse WD repeat-containing protein 5 (Wdr5)

Recombinant Mouse WD repeat-containing protein 5 (Wdr5)

  • 中文名称：
    小鼠Wdr5重组蛋白
  • 货号：
    CSB-YP026038MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Wdr5重组蛋白
  • 货号：
    CSB-EP026038MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Wdr5重组蛋白
  • 货号：
    CSB-EP026038MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Wdr5重组蛋白
  • 货号：
    CSB-BP026038MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Wdr5重组蛋白
  • 货号：
    CSB-MP026038MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Wdr5
  • Uniprot No.：
  • 别名：
    Wdr5; Big; Big3WD repeat-containing protein 5; BMP2-induced 3-kb gene protein; WD repeat-containing protein BIG-3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-334
  • 氨基酸序列
    MATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWL ASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGK CLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFN RDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKL WDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGH TDVVISTACHPTENIIASAALENDKTIKLWKSDC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation. In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.
  • 基因功能参考文献：
    1. Cbx8 associates with non-PRC1 complexes containing the H3K4 methyltransferase complex component WDR5, which together regulate Notch gene expression. PMID: 27346354
    2. Wdr5 suppression also reduced IFNgamma, Il-1beta, and TNFalpha induced chemokine gene expression in b-cells without affecting islet-cell survival or b-cell function after 48hrs, but did begin to increase islet-cell apoptosis PMID: 26505193
    3. results suggest that specific RNAs are integral inputs into the WDR5-MLL complex for maintenance of the active chromatin state and embryonic stem cell fates PMID: 24521543
    4. Data show that broad association of myc proto-oncogene protein MYC with chromatin depends on interaction with the WD40-repeat protein WDR5. PMID: 25818646
    5. BIG3 inhibits insulin granule biogenesis and insulin secretion. PMID: 24711543
    6. BIG3 negatively regulates insulin granule exocytosis by restricting insulin granule biogenesis without the release kinetics of individual granules at the final exocytotic steps being affected. PMID: 25139048
    7. Depletion of KAT2B or WDR5 decreased gluconeogenic gene expression. PMID: 24051374
    8. analysis of a novel mechanism for epigenetic control of SMC-marker gene expression during development through interaction of WDR5, homeodomain proteins, and chromatin remodeling enzymes PMID: 21531708
    9. Study shows that WD repeat domain 5 (Wdr5), a core member of the mammalian Trithorax (trxG) complex, positively correlates with the undifferentiated state and is a regulator of ES cell self-renewal. PMID: 21477851
    10. Characterization of a novel WDR5-binding site that recruits RbBP5 through a conserved motif to enhance methylation of histone H3 lysine 4 by mixed lineage leukemia protein-1. PMID: 20716525
    11. WDR5 is essential in assembling a virus-induced VISA-associated complex and plays an important role in virus-triggered induction of type I interferons. PMID: 20080758
    12. BIG-3 accelerates chondrocyte differentiation in vitro, and is expressed in the growth plate during embryonic development, so is is likely to play an in vivo regulatory role in the developing growth plate. PMID: 14657013
    13. identified two proteins, NONO and WDR5, that can associate with the mammalian PER1 protein; WDR5 augmented PER-mediated transcriptional repression, and its reduction by RNAi diminished circadian histone methylations at the promoter of a clock gene PMID: 15860628
    14. Wdr5 accelerates osteoblast differentiation in association with activation of the canonical Wnt pathway. PMID: 16730692
    15. Wdr5 is present on the Wnt1 promoter and on canonical Wnt response elements of the c-myc and Runx-2 promoters. PMID: 18201971
    16. Perichondrium expression of Wdr5 regulates chondrocyte proliferation and differentiation. PMID: 19217897

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    WD repeat WDR5/wds family
  • 组织特异性：
    Expressed in liver (at protein level). Detected in brain, testis and kidney.
  • 数据库链接：

    KEGG: mmu:140858

    STRING: 10090.ENSMUSP00000109585

    UniGene: Mm.28265