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  4. Recombinant Mouse WAS/WASL-interacting protein family member 1 (Wipf1)

Recombinant Mouse WAS/WASL-interacting protein family member 1 (Wipf1)

  • 货号：
    CSB-YP812968MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP812968MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP812968MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP812968MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP812968MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Wipf1; Waspip; Wip; WAS/WASL-interacting protein family member 1; Wiskott-Aldrich syndrome protein-interacting protein; WASP-interacting protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-493
  • 氨基酸序列
    MPVPPPPAPP PPPTFALANT EKPTLNKTEQ AGRNALLSDI SKGKKLKKTV TNDRSAPILD KPKGAGASAG GYGGGGGGGG GGGGGGGGSG GNFGGGGPPG LGGLFQAGMP KLRSTANRDN DSGGSRPPIL PPGGRATSAK PFSPPSGPGR FPAPSPGHRS GPPEPPRNRM PPPRPDVGSK PDSLPPPVPN TPRPVPSSLH NRGSPAGLGA PRPPFPGNRG AAFGAGSARQ NPSGSSSPFP RPPLPPTPSR ALDDKPPPPP PPVGNRPSMH REAVPPPPSQ TSKPPVPSTP RPGLGSQAPP PPPPPSRPGP PPLPPASNDE IPRLPQRNLS LTSSAPPLPS PGRSGPLPPP PSERPPPPVR DPPGRSGPLP PPPPINRNGS TARALPATPQ LPSRSGMDSP RSGPRPPLPP DRPGAGAPPP PPPSTSVRNG FQDSSCEDEW ESRFYFHPIS DLPPPEPYVP TTKTYPSKLA RNESRSGSNR RERGAPPLPP IPR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation. Plays a role in the formation of cell ruffles.
  • 基因功能参考文献：
    1. These experiments identify WIP as a member of a signaling cascade comprised of Abl family kinases, mTORC1 and S6K, which regulates neuron development and specifically, neuritic branching and complexity. PMID: 26664784
    2. WIP is a link between membrane lipid composition and actin cytoskeleton at dendritic spines. PMID: 24698977
    3. WIPf1 deficiency results in defective B cell function. By regulating the cortical actin cytoskeleton, WIPf1 influences the function of CD19 as a general hub for PI3K signaling. PMID: 26453379
    4. WIP binding to actin, independently of its binding to Wiskott-Aldrich syndrome protein, is critical for the integrity of the actin cytoskeleton in T cells and for their migration into tissues. PMID: 25246631
    5. Results show that WIP is a novel regulator of focal adhesion assembly and cell adhesion. PMID: 24797074
    6. Data indicate the involvement of WIP (WASP Interacting Protein) in the control of migratory persistence in both mesenchymal (fibroblast) and amoeboid (B lymphocytes) motility. PMID: 23950925
    7. This study implicates WIP in enteropathogenic Escherichia coli-mediated actin polymerization and pedestal elongation. PMID: 22372637
    8. These findings identify a novel role for mAbp1 in growth factor-induced dorsal ruffle formation through its interaction with WIP. PMID: 19910490
    9. WIP participates in the actin reorganization that leads to ruffle formation. PMID: 12724353
    10. WIP regulates FcepsilonRI-mediated mast cell activation by regulating Syk levels and actin cytoskeleton rearrangement. PMID: 14757742
    11. results suggest that WASP and WIP are not essential for T lymphocyte rolling and adhesion, but play important and partially redundant roles in T cell chemotaxis in vitro and homing in vivo and function downstream of small GTPases PMID: 16141245
    12. WIP acts as a negative regulator of fibroblast migration. PMID: 17008118
    13. WASP-interacting protein stabilizes Wiskott-Aldrich syndrome protein(WASP) and suggest that it may also be important for its function PMID: 17213309
    14. These results indicate that WIP is essential for IL-2 signaling and responsiveness in T cells, possibly because of its critical role in TCR-triggered actin cytoskeletal reorganization. PMID: 19359486
    15. platelet-associated IgAs negatively modulate glycoprotein VI signaling and function in Wiskott-Aldrich syndrome protein-interacting protein KO mice. PMID: 19692704

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  • 亚细胞定位：
    Cytoplasmic vesicle. Cytoplasm, cytoskeleton. Cell projection, ruffle.
  • 蛋白家族：
    Verprolin family
  • 数据库链接：

    KEGG: mmu:215280

    STRING: 10090.ENSMUSP00000092268

    UniGene: Mm.223504