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  4. Recombinant Mouse Upstream stimulatory factor 1 (Usf1)

Recombinant Mouse Upstream stimulatory factor 1 (Usf1)

  • 中文名称：
    小鼠Usf1重组蛋白
  • 货号：
    CSB-YP717194MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Usf1重组蛋白
  • 货号：
    CSB-EP717194MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Usf1重组蛋白
  • 货号：
    CSB-EP717194MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Usf1重组蛋白
  • 货号：
    CSB-BP717194MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Usf1重组蛋白
  • 货号：
    CSB-MP717194MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Usf1
  • Uniprot No.：
  • 别名：
    Usf1; Usf; Upstream stimulatory factor 1; Major late transcription factor 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-310
  • 氨基酸序列
    MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV FRTENGGQVM YRVIQVSEGQ LDGQTEGSGA ISGYPATQSM TQAVIQGAFT SDDAVDTEGA AAETHYTYFP STAVGDGSGG TTSGSTTAVV TTQGSEALLG QATPPSTGQF FVMMSPQEVL QGGSQRSIAP RTHPYSPKSE APRTTRDEKR RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK GGILSKACDY IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG LEVVIKNDSN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
  • 基因功能参考文献：
    1. Mice lacking Usf1 displayed increased BAT-facilitated, diet-induced thermogenesis with up-regulation of mitochondrial respiratory chain complexes, as well as increased BAT activity even at thermoneutrality and after BAT sympathectomy PMID: 26819196
    2. USF1 exclusively bound to the CACGTG E-box motifs in the brain cortex neuron proximal promoter regions. Importantly, functional annotation of the USF1-binding targets revealed an enrichment of genes related to lysosomal functions. PMID: 26756308
    3. Propose that USF1 is an important modulator of molecular and behavioral circadian rhythms in mammals via DNA-binding of the CLOCK:BMAL1 complex. PMID: 23580255
    4. USF1 but not USF2 supports OPN expression in LRP6-VKO vascular smooth muscle lineage, and immunoprecipitation confirmed increased USF1 association with OPN chromatin. PMID: 26034040
    5. Pdx1, USF1 and USF2 co-ordinately regulate Alx3 gene expression in pancreatic beta-cells. PMID: 25040025
    6. USF1-centered regulatory networks were further confirmed by ChIP assays. PMID: 24686121
    7. AGTRAP expression is regulated by USF1 and USF2 PMID: 23653383
    8. USF1 transactivates GATA5 expression by binding to the E-box in its promoter PMID: 22625849
    9. Data suggest that both USF1 and USF2 are essential for angiotensinogen (AGT) transcriptional regulation, and distinct sex-specific and tissue-specific mechanisms are involved in the activities of these transcription factors. PMID: 22547438
    10. using a mouse model we show that the loss of USF-1 compromises DNA repair, which suggests that USF-1 plays an important role in maintaining genomic stability. PMID: 22291606
    11. USF-1 binds the 5'PDbeta2 repressor element (E-box) in developing double-negative thymocytes. PMID: 22287717
    12. critical transcription factor regulating diabetic kidney disease and plays a critical role in albuminuria, mesangial matrix accumulation, and TGF-beta1 and renin stimulation in diabetic kidney disease PMID: 21543418
    13. USF regulates osteopontin gene transcription aortic vascular smooth muscle cells, entrained to changes in cellular glucose metabolism PMID: 12200434
    14. USF binding to the E-box at -893/-888 serves to trans-repress basal expression and IL-1beta induction of the iNOS promoter. PMID: 12225970
    15. USF1, along with ikaros-2, regulates delta opioid receptor expression in mouse T lymphocytes PMID: 12431989
    16. Data show that USF1/-2 and CREB transcription factors binding to the cyclooxygenase-2 promoter induced H. pylori-dependent cox-2 transcription. PMID: 14531897
    17. UV-induced expression of POMC and MC1R is dependent on the p-38-activated upstream stimulating factor-1 PMID: 15358786
    18. Usf1, Runx1, and c-Myb play important roles as Edelta-dependent regulators of Ddelta2 accessibility in vivo PMID: 15778374
    19. USF1 is stimulated by modest increases in glucose concentration in murine mesangial cells and binds to the murine TGF-beta1 promoter. PMID: 15983197
    20. USF-1 is the major E-box-binding transcription factor regulating the Prnd promoter. PMID: 16297464
    21. USF1 and USF2 bind the Fshr promoter and revealed differences between Sertoli and granulosa cells in compensatory responses to USF loss and the USF dimeric composition required for Fshr transcription PMID: 18566134
    22. Data show that retinoic acid utilizes CREB and USF1 in a transcriptional feed-forward loop in order to stimulate MKP1 expression in human immunodeficiency virus-infected podocytes. PMID: 18625721
    23. In this study they asked whether the interactions between USF1/USF2 and E-Box and Nrf1 and GC-Box in the brain of mouse are influenced by postnatal age and what link it has with reported age-related decrease in the expression of Fmr-1 gene. PMID: 18782566
    24. USF1 functionally and differentially regulates AGT expression via the -20 polymorphism and that the differential expression exhibited by -20 can be accounted for by differential association with USF1. PMID: 18802024
    25. USF-1 specificity is directed via p38-mediated phosphorylation-dependent acetylation PMID: 19389701
    26. USF regulates globin gene expression indirectly by enhancing the expression of erythroid transcription factors and directly by mediating the recruitment of transcription complexes to the globin gene locus. PMID: 19704006

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: mmu:22278

    STRING: 10090.ENSMUSP00000125729

    UniGene: Mm.8