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  4. Recombinant Mouse Tyrosine-protein kinase SYK (Syk)

Recombinant Mouse Tyrosine-protein kinase SYK (Syk)

  • 中文名称：
    小鼠Syk重组蛋白
  • 货号：
    CSB-YP022998MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Syk重组蛋白
  • 货号：
    CSB-EP022998MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Syk重组蛋白
  • 货号：
    CSB-EP022998MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Syk重组蛋白
  • 货号：
    CSB-BP022998MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Syk重组蛋白
  • 货号：
    CSB-MP022998MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Syk; ptk72; SykbTyrosine-protein kinase SYK; EC 2.7.10.2; Spleen tyrosine kinase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-629
  • 氨基酸序列
    MAGSAVDSAN HLTYFFGNIT REEAEDYLVQ GGMTDGLYLL RQSRNYLGGF ALSVAHNRKA HHYTIERELN GTYAISGGRA HASPADLCHY HSQEPDGLIC LLKKPFNRPP GVQPKTGPFE DLKENLIREY VKQTWNLQGQ ALEQAIISQK PQLEKLIATT AHEKMPWFHG NISRDESEQT VLIGSKTNGK FLIRARDNSG SYALCLLHEG KVLHYRIDRD KTGKLSIPEG KKFDTLWQLV EHYSYKPDGL LRVLTVPCQK IGAQMGHPGS PNAHPVTWSP GGIISRIKSY SFPKPGHKKP APPQGSRPES TVSFNPYEPT GGPWGPDRGL QREALPMDTE VYESPYADPE EIRPKEVYLD RSLLTLEDNE LGSGNFGTVK KGYYQMKKVV KTVAVKILKN EANDPALKDE LLAEANVMQQ LDNPYIVRMI GICEAESWML VMEMAELGPL NKYLQQNRHI KDKNIIELVH QVSMGMKYLE ESNFVHRDLA ARNVLLVTQH YAKISDFGLS KALRADENYY KAQTHGKWPV KWYAPECINY YKFSSKSDVW SFGVLMWEAF SYGQKPYRGM KGSEVTAMLE KGERMGCPAG CPREMYDLMN LCWTYDVENR PGFTAVELRL RNYYYDVVN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Required for the stimulation of neutrophil phagocytosis by IL15. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Involved in interleukin-3/IL3-mediated signaling pathway in basophils. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen. Together with CEACAM20, enhances production of the cytokine CXCL8/IL-8 via the NFKB pathway and may thus have a role in the intestinal immune response.
  • 基因功能参考文献：
    1. Cryptotanshinone inhibits IgEmediated degranulation through inhibition of spleen tyrosine kinase and tyrosineprotein kinase phosphorylation in mast cells. PMID: 29845271
    2. Results indicate spleen tyrosine kinase (SYK) as an essential mediator of brown fat formation and function. PMID: 29235464
    3. CD11b promotes the differentiation of osteoclasts induced by RANKL through the Syk signaling pathway. PMID: 28661042
    4. Phosphorylation levels of 10 phosphosites in spleen tyrosine kinase (SYK) were increased following B cell receptor activation. 4 cluster regions, encompassing 8 phosphosites, were activated by mercury. Phosphorylation of tyrosine-342 and -346 residues were most sensitive to mercury exposure. PMID: 28716125
    5. Comparison of the distributions of tau pTyr18 and double-phosphorylated Syk in the transgenic mouse brain and human hippocampus showed that the phosphorylation of tyrosine 18 in tau already occurs at an early stage of tauopathy and increases with the progression of neurodegeneration. Syk appears unlikely to be a major kinase that phosphorylates tyrosine 18 of tau at the early stage of tauopathy. PMID: 28919467
    6. Syk kinase acts downstream of receptors controlling granule cells tangential migration PMID: 28768156
    7. Results show that Syk activation occurs following Abeta deposition and the formation of tau pathological species. As Syk activation also promotes Abeta formation and tau hyperphosphorylation, results suggest that Alzheimer's disease pathological lesions may be self-propagating via a Syk dependent mechanism highlighting Syk as an attractive therapeutic target for the treatment of Alzheimer's disease. PMID: 28877763
    8. Syk-induced signals in bone marrow stromal cell lines are mediated by phospholipase C gamma1 (PLCgamma1) in osteogenesis and PLCgamma2 in adipogenesis. PMID: 28786489
    9. Study demonstrates that redundant complexes formed by the mutant SYK(Y3F) protein allow it to maintain signaling to receptor-proximal interaction partners, resulting in proper initiation of BCR-proximal signals. PMID: 28760774
    10. SYK could induce HO-1 expression by regulating the Akt phosphorylation level in H9c2 with A/H injury, protecting H9c2 from the injury induced by A/H treatment. PMID: 28869869
    11. These results identify Syk as a critical component of skin inflammation in a mouse model of epidermolysis bullosa acquisita PMID: 28576735
    12. Syk was identified as the target enzyme most strongly inhibited by water extract of Cordyceps bassiana. PMID: 28830213
    13. The downmodulation of Syk expression and phosphorylation in intestinal DCs could be important mechanisms through which helminths induce regulatory DCs that limit colitis. PMID: 27559049
    14. ephrin B3/EphB2 are obvious candidates for driving the Syk-dependent repulsive response. PMID: 27122172
    15. IgG (mAb)-opsonized, inactivated Francisella tularensis LVS enhances macrophage and dendritic cell IL-1beta responses in a TLR2- and FcgammaR-dependent fashion. FcgammaR-mediated Syk activation leads to NLRP3 inflammasome-dependent IL-1beta production in macrophages. PMID: 27365531
    16. The effect of Smad4 was at least partially mediated by the downstream effectors Syk and ROCK2 transcription in megakaryocytes. PMID: 28663280
    17. Gene expression analysis of lung tissues revealed that HDM-induced expression of IL-17 and CXCL-1 was significantly attenuated in both Sykdel/del and GSK143-treated mice. CONCLUSION: Syk inhibitors may play a role in the management of neutrophilic asthma PMID: 28107345
    18. collagen-I-mediated inhibition of proplatelet formation is specifically controlled by GPVI. PMID: 27505889
    19. Pharmacological Syk inhibition might provide a safe therapeutic strategy to prevent arterial thrombosis and to limit infarct progression in acute stroke. PMID: 27102960
    20. Studies indicate that SHP1 and SYK crosstalk as a critical regulator of MyD88 post-translational modifications and IL-1-driven inflammation. PMID: 28410990
    21. dephosphorylation of Tyr(P)(346) may be considered an important "checkpoint" in the regulation of Syk activation process. Putative biological functions of TULA-2-mediated dephosphorylation of Tyr(P)(346) may include deactivation of receptor-activated Syk or suppression of Syk activation by suboptimal stimulation. PMID: 27609517
    22. These studies define a pathologic role for myeloid Syk signaling in renal ischemia/reperfusion injury PMID: 27322771
    23. Torreya nucifera butanol fraction exhibits anti-inflammatory activities by direct inhibition of macrophage Src/Syk/NF-kappaB and IRAK1/AP-1 signaling. PMID: 27178629
    24. Dectin-1 and Dectin-2 seem not to play a major role in Borrelia recognition or Borrelia-induced inflammation. However, Syk seems to be involved in Borrelia-induced cytokine production PMID: 26297607
    25. TNF activates Mule by inducing the dissociation of Mule from its inhibitor ARF. Inhibition of Mule phosphorylation by silencing Syk prevents this, thereby inhibiting Mule E3 ligase activity and TNF-induced JNK activation and cell death. PMID: 26212014
    26. The effects of OXSI-2 identified in this study provide context for the role of Syk in inflammasome signaling and demonstrate its importance in oxidative signaling upstream of inflammasome activation and downstream of ion flux. PMID: 26970308
    27. Mincle Activation and the Syk/Card9 Signaling Axis Are Central to the Development of Autoimmune Disease of the Eye PMID: 26921309
    28. Syk deletion in myeloid cells is protective in mouse nephrotoxic serum nephritis. PMID: 26251216
    29. results show that cells of neuronal origin adapt their proteome to exogenous alpha-Syn fibrils and actively modify those assemblies PMID: 26708990
    30. These results provide new insights into the mechanisms by which SYK regulates MHC-II expression via autophagy in macrophages. PMID: 25946330
    31. Syk mediates airway contractility in the normal and allergic airways, independent of its role and function in leukocytes, and supports a paracrine role for airway epithelial Syk in modulating airway smooth muscle activity. PMID: 25556883
    32. Syk expression by DCs is necessary for IL-23p19 production in response to C. albicans PMID: 25033445
    33. The Syk inhibitor fostamatinib prevented bleomycin-induced fibrosis and inflammation in the skin and in the lung. The anti-fibrotic effect of fostamatinib is linked to reduced Syk phosphorylation and TGF-beta expression. PMID: 26148346
    34. PRT-060318 had a significant, albeit partial, inhibitory effect, thus suggesting that ROS induce NET formation in part via activation of Syk. PMID: 25277753
    35. Together these data reveal a mechanism by which SHP-2 mediates the activation of Syk in response to fungal infection. PMID: 25915733
    36. These experiments demonstrate that Syk plays a critical role in multiple aspects of B cell antibody responses. PMID: 25862820
    37. PI3K and Tec family kinases play a crucial role in the regulation of platelet activation and Syk phosphorylation downstream of the CLEC-2 receptor PMID: 25767114
    38. Syk-deficient B cells require BAFF receptor and CD19/PI3K signaling for their long-term survival. PMID: 25630702
    39. Rapid CD4+ T-cell responses to bacterial flagellin require dendritic cell expression of Syk and CARD9. PMID: 25430631
    40. alphaIIbbeta3 regulates Syk through an ITAM-independent pathway in mice and provide novel insight into the course of events underlying Syk activation and hemITAM phosphorylation by CLEC-2 PMID: 25352128
    41. Polyoxygenated cholesterol ester hydroperoxide-induced and TLR4/SYK-mediated macrophage responses may contribute to chronic inflammation in atherosclerosis. PMID: 24376657
    42. deregulated Syk activity by itself is not sufficient for the transformation of B cells, as downstream effectors, such as Blimp-1, limit the survival and expansion of the activated B cell PMID: 23955076
    43. in DKO pre-B cells, the kinase Zap70 is associated with the pre-BCR, suggesting that Zap70 is important to promote B cell maturation in the absence of Syk and SHP-1. PMID: 24899508
    44. Non-receptor protein tyrosine kinase signaling pathways including Syk/NF-kappaB and Jak2/Stat3 cascades are potential anti-neuroinflammatory targets in microglia. PMID: 24486434
    45. Data indicate that phosphorylation of Tyr114 and Tyr259 of prohibitin (PHB) is critical for the association of PHB with FcRI and Syk. PMID: 24023254
    46. results demonstrate the opposing regulatory roles of Syk in TLR-mediated TRAF6 and TRAF3 signaling pathways, which suggests that Syk may fine-tune the innate immune response to lessen inflammation. PMID: 23962979
    47. Data indicate that ASC was phosphorylated during inflammasome activation in a Syk- and Jnk-dependent manner. PMID: 24185614
    48. Syk activates PI3K/Akt signaling which is required for survival of EBV+ B cell lymphomas PMID: 23398911
    49. PKCbeta is the isoform responsible for Syk negative regulation PMID: 23960082
    50. while the kinase domain of Syk is uniquely required for OC function that of Zap70 inhibits it. PMID: 23494777

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  • 亚细胞定位：
    Cell membrane. Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, SYK/ZAP-70 subfamily
  • 数据库链接：

    KEGG: mmu:20963

    STRING: 10090.ENSMUSP00000060828

    UniGene: Mm.375031