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  4. Recombinant Mouse Tyrosine-protein kinase CSK (Csk)

Recombinant Mouse Tyrosine-protein kinase CSK (Csk)

  • 中文名称：
    Recombinant Mouse Tyrosine-protein kinase CSK(Csk),Yeast
  • 货号：
    CSB-YP006056MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Tyrosine-protein kinase CSK(Csk),Yeast
  • 货号：
    CSB-EP006056MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Tyrosine-protein kinase CSK(Csk),Yeast
  • 货号：
    CSB-EP006056MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Tyrosine-protein kinase CSK(Csk),Yeast
  • 货号：
    CSB-BP006056MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Tyrosine-protein kinase CSK(Csk),Yeast
  • 货号：
    CSB-MP006056MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Csk
  • Uniprot No.：
  • 别名：
    CskTyrosine-protein kinase CSK; EC 2.7.10.2; C-Src kinase; Protein-tyrosine kinase MPK-2; p50CSK
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-450
  • 氨基酸序列
    SAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLYPPE TGLFLVREST NYPGDYTLCV SCEGKVEHYR IMYHASKLSI DEEVYFENLM QLVEHYTTDA DGLCTRLIKP KVMEGTVAAQ DEFYRSGWAL NMKELKLLQT IGKGEFGDVM LGDYRGNKVA VKCIKNDATA QAFLAEASVM TQLRHSNLVQ LLGVIVEEKG GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE AMEYLEGNNF VHRDLAARNV LVSEDNVAKV SDFGLTKEAS STQDTGKLPV KWTAPEALRE KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDAPDG CPPAVYEVMK NCWHLDAATR PTFLQLREQL EHIKTHELHL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
  • 基因功能参考文献：
    1. Our results have uncovered a previously unknown role of c-Src tyrosine kinase in regulating interneuron function in the brain and identified a novel mechanism by which TASK-1 channels are activated in neurons. PMID: 28714121
    2. in the absence of PAG, Csk becomes more associated with alternative partners; i.e., phosphatase PTPN22 and Dok adaptors. Combining PAG deficiency with PTPN22 or Dok adaptor deficiency further enhances effector T cell responses. Unlike PAG, Cbl ubiquitin ligases inhibit the activation of naive, but not of effector, T cells. PMID: 27926878
    3. Csk is a causative gene in the 15q24 locus and regulates blood pressure through Src, and these findings provide a novel therapeutic target for the treatment of hypertension PMID: 26751575
    4. Csk plays an important role, not only in basal signaling, but also in setting the T cell antigen receptor signaling threshold and affinity recognition. PMID: 26302204
    5. SHIP1 and Csk are part of the PSTPIP2-dependent inhibitory network that prevents the development of autoinflammatory disease. PMID: 26304991
    6. If Cbp enhances the interaction between c-Src and FAK, Cbp could promote c-Src function when lipid rafts are disrupted. PMID: 24675741
    7. Differentiation of embryonic stem cells is driven by c-Src is antagonized by c-Yes. PMID: 23895624
    8. critical role in preventing TCR signaling PMID: 24317039
    9. It was concluded that c-Src and NADPH oxidase components are necessary for redox-mediated production of TNF-alpha following liver ischemia-reperfusion and that hepatocytes play an important role in this process. PMID: 23639811
    10. Data indicate that chemical rescue of c-Src provided a tool to dissect the spatiotemporal mechanism of activation of the Rap1 guanine exchange factor, C3G, one of the identified potential c-Src substrates that plays a role in focal adhesion signaling. PMID: 22499769
    11. The Csk pathway appears to be a critical signaling cascade regulating social learning and memory PMID: 22348736
    12. These data demonstrate that expression of an activated allele of c-src overcomes the block to mammary gland morphogenesis in mice arising due to the loss of the cytoplasmic C-terminal domain of Ptch1. PMID: 22968113
    13. Data show that the phosphoprotein associated with glycosphigolipid-enriched microdomains (PAG)/Csk binding protein (Cbp) modulates SFK activity in the brain. PMID: 21915273
    14. Caveolin-1 and C-terminal Src kinase binding protein are cooperative, and increases in their expression or phosphorylation favors repression of C-terminal Src kinase activity. PMID: 21778303
    15. expression of Cbp is downregulated during osteoclast differentiation, consequently inhibiting recruitment of Csk to lipid rafts and keeping c-Src activity high in osteoclasts. findings provide a novel paradigm that controls c-Src activity in osteoclasts. PMID: 19874196
    16. show that relative titration of CD45 and Csk expression reveals distinct regulation of basal and inducible T cell receptor signaling during thymic development. PMID: 20346773
    17. Results identify Src-family kinase substrates involved in platelet-derived growth factor signaling. PMID: 19632164
    18. the absence of c-src expression indeed affects the development of selected, TCDD-induced toxic endpoints that are related to wasting syndrome PMID: 11882352
    19. role for Csk in P. aeruginosa invasion, while providing further evidence that actin cytoskeleton disruption contributes to ExsA-regulated P. aeruginosa cytotoxicity and invasion inhibition PMID: 12220990
    20. integrin-mediated signals in Csk-deficient fibroblasts (Csk(-) cells) and their stable transfectants expressing re-introduced Csk (Csk(-)/Csk cells) PMID: 12670507
    21. the loss of IGF-I downstream mitogenic signaling in differentiating 3T3-L1 cells is due to a change in IGF-I activation of c-Src and CSK may mediate the inactivation of c-Src by IGF-I in 3T3-L1 adipogenesis. PMID: 12746317
    22. Csk is required both for angiogenic sprouting and vascular remodeling. PMID: 14576066
    23. role in regulating the permissiveness for internalization of the alphaPDGFR PMID: 14644164
    24. Disruption of Ca(2+)-dependent cell matrix adhesion enhances Csk activity, but causes disssociation of the c-sarc/FAK complex and dephosphorylation of tyrosine of FAK in carcinoma cells. PMID: 14729052
    25. In adult testis, however, a differential Csk immunoreactivity was found among the seminiferous tubules. PMID: 15277007
    26. Csk is a critical regulator of TLR4-mediated signalling by modifying the levels of Src-like kinases. PMID: 15773898
    27. Csk has a critical role in cell migration PMID: 16501257
    28. Our data demonstrate that Csk is involved in the control of cGMP levels and that membrane-bound guanylyl cyclases can be critically modulated by other receptor-initiated intracellular signaling pathways. PMID: 16519534
    29. Osteoclastic protein-tyrosine phosphatase (PTP-oc) activates osteoclast activity in part by promoting osteoclast survival through the PTP-oc-mediated c-Src-dependent activation of NFkappaB and JNK2. PMID: 17400176
    30. Epidermal hyperplasia and hyperkeratosis in Csk-knockoutt mice were associated with an increased number of stem cells in the interfollicular epidermis and chronic inflammation in dermis and epidermal hyperplasia. PMID: 17494055
    31. Whereas Ang II has an obligatory need for c-Src, ET-1 mediates its actions through a c-Src-independent Ras-Raf-dependent pathway for MAPK activation PMID: 17569879
    32. In conclusion, suppression of c-Src activity stimulates muscle differentiation by activating p38 MAPK uni-directionally. PMID: 17612500
    33. Src is an essential regulator of podosome structure, dynamics and organization. PMID: 17978100
    34. While Gab2 co-operates with overexpressed c-Src to enhance proliferative signaling, co-expression of Gab2 with activated Src mutants promotes acinar disruption. PMID: 17998934
    35. These results suggest novel roles for ZO proteins as Src/Csk scaffolds potentially involved in the regulation of Src transformation. PMID: 18086565
    36. c-Src has the potential to induce cell transformation, but it requires coordination with an additional pathway(s) to promote tumor progression in vivo. PMID: 18173743
    37. Inactivation of Csk caused deformities in various tissues of neural crest origins, including facial dysplasia and corneal opacity. PMID: 18262517
    38. PLCgamma2 influences Src activation by mediating the localization of Src to the integrin complex and thereby regulating integrin-mediated functions in the osteoclast. PMID: 18378693
    39. These findings indicate a potential role for Cbp as a suppressor of c-Src-mediated tumor progression. PMID: 18498747
    40. Activation of c-Src and Fyn kinases by protein-tyrosine phosphatase RPTPalpha is substrate-specific and compatible with lipid raft localization. PMID: 18948260
    41. Neutrophil transendothelial migration induced by septic alveolar macrophages is dependent on the generation of superoxide in endothelial cells via the c-Src kinase/NADPH oxidase signaling pathway. PMID: 19050294
    42. The expression and activity of Src and low molecular weight protein tyrosine phosphatase (LMWPTP) during osteoblast differentiation was examined and the modulation of Src kinase by LMWPTP was assessed. PMID: 19088431
    43. six genes (Csk, Itga3, Jarid2, Prkaca, Rarg, and Sall4), known to cause NTDs when deleted, that are also down-regulated in p53 -/- embryos. PMID: 19229884
    44. Glucose 6-phosphate dehydrogenase is regulated through c-Src-mediated tyrosine phosphorylation in endothelial cells. PMID: 19359662
    45. c-Src enhances RIG-I-mediated signaling, acting at the level of TRAF3 PMID: 19419966
    46. Csk-mediated tyrosine phosphorylation is a novel molecular mechanism to limit P2X3 receptor function in mouse sensory neurons PMID: 19509283
    47. that distinct SFK signaling pathways regulate mES cell fate; the formation of primitive ectoderm is regulated by the activity of c-Src PMID: 19825829
    48. Anti-inflammatory role through control of the neutrophil activation threshold in vivo: suppression of premature degranulation and integrin release, neutrophil recruitment and adhesion, while supporting cell migration. PMID: 14975240

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Note=Mainly cytoplasmic, also present in lipid rafts.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, CSK subfamily
  • 组织特异性：
    Ubiquitous, but most abundant in thymus and spleen, as well as in neonatal brain.
  • 数据库链接：

    KEGG: mmu:12988

    STRING: 10090.ENSMUSP00000034863

    UniGene: Mm.21974