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  4. Recombinant Mouse Transcription factor E2F3 (E2f3)

Recombinant Mouse Transcription factor E2F3 (E2f3)

  • 货号：
    CSB-YP007342MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007342MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007342MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007342MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007342MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    E2f3; Transcription factor E2F3; E2F-3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-457
  • 氨基酸序列
    MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAP GTYIQILTTN PSTTSCATSL QSGALTAGPL LPSVPGTEPA ASSLYTTPQG PSSRVGLLQQ PPAPGRGGGG GPPAKRRLEL GESGHQYLSD GLKTPKGKGR AALRSPDSPK TPKSPSEKTR YDTSLGLLTK KFIQLLSQSP DGVLDLNKAA EVLKVQKRRI YDITNVLEGI HLIKKKSKNN VQWMGCSLSE DGGMLAQCQG LSKEVTELSQ EEKKLDELIQ SCTLDLKLLT EDSENQRLAY VTYQDIRKIS GLKDQTVIVV KAPPETRLEV PDSIESLQIH LASTQGPIEV YLCPEETETH RPMKTNNQDH NGNIPKPTSK DLASNNSGHS DCSVSTANLS PLASPANLLQ QTEDQIPSNL EGPFVNLLPP LLQEDYLLSL GEEEGISDLF DAYDLEKLPL VEDFMCS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters.
  • 基因功能参考文献：
    1. Findings identify E2f3 as a key transcription factor in tumor-associated macrophages (TAMs), which influences the tumor microenvironment and tumor cell metastasis. PMID: 26549026
    2. Data show that overexpression of microRNA miR-17-92 in differentiated C2C12 myoblast cells dramatically reduced the expression of E2F3b protein. PMID: 28362317
    3. Data indicate that microRNA miR-125a-5p and E2F3 transcription factor display reversely associated expression pattern in the proliferative phase of C2C12 myoblasts. PMID: 25733534
    4. Oct3/4 directly bound to the promoter region of the E2F3a gene and positively regulated expression of E2F3a in embryonic stem cells. PMID: 25727014
    5. These results point to RB as a key regulator of SC function in adult mice and that the RB/E2F3 pathway directs SC maturation, cell cycle quiescence, and RB protects SC from apoptosis. PMID: 24901045
    6. E2F3 promotes while E2F2 suppresses ischemic cardiac repair through corresponding changes in endothelial cell proliferation. PMID: 23799044
    7. E2F2 impairs, and endothelial E2F3 promotes, the angiogenic response to peripheral ischemic injury through corresponding changes in EC cell-cycle progression. PMID: 23603666
    8. E2f3a and E2f3b differentially regulate Sox2 dosage in neural precursors. PMID: 23499385
    9. down-regulation of E2f3 with age helps drive the dramatic decline in Igf2 expression in postnatal organs, and E2F3 overexpression in cancer induces IGF2 overexpression PMID: 23530192
    10. identification of EGFR-independent mechanisms in E2F3a control can be helpful in explaining the non-responsiveness of therapeutic EGFR targeting in ovarian cancer. PMID: 21516127
    11. These findings show direct regulation of neogenin by the Rb/E2F3 pathway and demonstrate that regulation of neogenin expression is required for neural precursor migration. PMID: 21059867
    12. the hypoxia component of ischemia may limit wound re-epithelialization by stabilizing HIF-1alpha, which induces miR-210 expression, resulting in the down-regulation of the cell-cycle regulatory protein E2F3 PMID: 20308562
    13. E2F3 reduces the unscheduled proliferation of fiber cells and in Rb-deficient retinas, contribute to the ectopic proliferation of postmitotic neuronal cells PMID: 12065245
    14. the E2F3 transcription factor as an important link that orchestrates DNA and centrosome duplication cycles, ensuring the faithful transmission of genetic material to daughter cells PMID: 12726860
    15. E2F3 loss leads to an increase in the rate of tumor initiation. Finally, analysis of Rb(+/-); E2f3(+/-) mice shows that this tumor-suppressive function of E2F3 is dose dependent. PMID: 12944480
    16. Deregulated E2F3 activity induces hyperplasia and senescence-like features in the pituitary gland PMID: 15767672
    17. E2F3a and E2F4, but not E2F5, function to induce cell cycle entry, although E2F4 has more modest activity. E2F3a may induce cell death primarily through activation of p73alpha. PMID: 15855772
    18. E2F3a has a unique apoptotic activity and lacks the tumor suppressive property of E2F1 in this model system. PMID: 16340309
    19. E2f3 is dispensable for the development of retinoblastoma 1-deficient pituitary and thyroid tumors PMID: 17210634
    20. This study not only outlines overlapping and distinct functions for E2Fs in neurogenesis but also is the first to establish a physiologically relevant role for the Rb/E2F pathway beyond cell cycle regulation in vivo. PMID: 17452454
    21. Rb regulates neural differentiation directly, and, unexpectedly, it involves inhibition of E2f3a, not potentiation of tissue-specific factors. PMID: 17608565
    22. The E2F transcription factors are key cell cycle regulators that play important roles in modulating cell proliferation and cell fate. PMID: 17652516
    23. requirement of E2F3 for BCR/ABL leukemogenesis PMID: 17925491
    24. deregulated expression of E2F3a causes DNA damage under physiological conditions and indicate that the ATM-dependent response to this damage is important for the induction of apoptosis and tumor suppression PMID: 18469863
    25. E2f3a is sufficient to support mouse embryonic and postnatal development PMID: 18594513
    26. E2f3a and E2f3b have largely overlapping functions in vivo and E2f3a can fully substitute for E2f1 and E2f3 in most murine tissues. PMID: 18663357
    27. study provides evidence suggesting E2F3a & E2F3b contribute to activation of E2F target gene expression & cell proliferation but E2F3a is the main isoform contributing to unscheduled proliferation, apoptosis & early lethality observed in Rb-/- embryos PMID: 19015245
    28. These data demonstrate a clear role for E2F3 in myocardial and cardiac function during both development and adulthood. PMID: 19029823
    29. E2f3b, but not E2f3a or other E2F family members, is required for myogenic differentiation, and that this requirement for E2f3b does not depend on retinoblastoma tumor suppressor family PMID: 19136625
    30. E2F2 and E2F3 had positive effects on the promoter activity of fibrillin-2 in chondrocyte differentiation PMID: 19142862
    31. These results support a model whereby p107/E2F3 regulates the pool of FGF-responsive progenitors by directly regulating FGF2 gene expression in vivo. PMID: 19564414

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    E2F/DP family
  • 数据库链接：

    KEGG: mmu:13557

    STRING: 10090.ENSMUSP00000100012

    UniGene: Mm.268356