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  4. Recombinant Mouse Telomeric repeat-binding factor 1 (Terf1)

Recombinant Mouse Telomeric repeat-binding factor 1 (Terf1)

  • 中文名称：
    小鼠Terf1重组蛋白
  • 货号：
    CSB-YP023386MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Terf1重组蛋白
  • 货号：
    CSB-EP023386MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Terf1重组蛋白
  • 货号：
    CSB-EP023386MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Terf1重组蛋白
  • 货号：
    CSB-BP023386MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Terf1重组蛋白
  • 货号：
    CSB-MP023386MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Terf1; Trf1Telomeric repeat-binding factor 1; TTAGGG repeat-binding factor 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-421
  • 氨基酸序列
    AETVSSAARDAPSREGWTDSDSPEQEEVGDDAELLQCQLQLGTPREMENAELVAEVEAVA AGWMLDFLCLSLCRAFRDGRSEDFRRTRDSAEAIIHGLHRLTAYQLKTVYICQFLTRVAS GKALDAQFEVDERITPLESALMIWNSIEKEHDKLHDEIKNLIKIQAVAVCMEIGSFKEAE EVFERIFGDPEFYTPLERKLLKIISQKDVFHSLFQHFSYSCMMEKIQSYVGDVLSEKSST FLMKAATKVVENEKARTQASKDRPDATNTGMDTEVGLNKEKSVNGQQSTETEPLVDTVSS IRSHKNALSQLKHRRAPSDFSRNEARTGTLQCETTMERNRRTSGRNRLCVSENQPDTDDK SGRRKRQTWLWEEDRILKCGVKKYGEGNWAKILSHYKFNNRTSVMLKDRWRTMKRLKLIS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • 基因功能参考文献：
    1. It plays a role in hematopoietic stem cell regulation and in the maintenance of the HSC pool and the development of hematological disorders. PMID: 29550946
    2. loss of telomere structure mediated by the TERB1-TRF1 interaction significantly affects homologous pairing of the telomere-adjacent pseudoautosomal region (PAR) of the X and Y chromosomes in mouse spermatocytes. PMID: 29083416
    3. Data show that TRF1 expression is increased in both human and mouse glioblastoma multiforme (GBM). PMID: 29136505
    4. full-length TRF1 presents a molecular architecture that assists its interaction with telometic DNA and at the same time makes TRFH domains accessible to other TRF1 binding partners. PMID: 27563064
    5. Gene dosage reductions of Trf1 and/or Tin2 induce telomere DNA damage and lymphoma formation in aging mice. PMID: 26135248
    6. TRF1 is exclusively located at telomeres under normal conditions and under extreme telomere shortening. PMID: 25483083
    7. Telomeric repeat-binding factor 1, a core component of the telomere protein complex, is a mediator of telomere associations in mammalian cells. PMID: 24829382
    8. TRF1 binds BLM to facilitate lagging but not leading strand telomeric DNA synthesis PMID: 25344324
    9. TRF1 is necessary for both induction and maintenance of pluripotency. PMID: 23735977
    10. Loss of TRF1 leads to bone marrow failure and recapitulates clinical features of dyskeratosis congenita. PMID: 22932806
    11. Mouse gene deletion experiments revealed DNA-damage-response pathways that threaten chromosome ends and how the components of the telomeric shelterin complex prevent activation of these pathways.[Shelterin] PMID: 21209389
    12. Identification of a tankyrase-binding motif shared by IRAP, TAB182, and human TRF1 but not mouse protein. PMID: 12080061
    13. Here we show that targeted deletion of exon 1 of the mouse gene encoding Trf1 causes early (day 5 to 6 postcoitus) embryonic lethality.These data suggest that murine Trf1 has an essential function that is independent of telomere length regulation PMID: 12944479
    14. TRF1 interacts with other telomere-binding molecules and integrates into the functional telomere structure PMID: 14559908
    15. identified mouse Telomeric Repeat Binding Factor 1 (Trf1) as a protein that interacts directly with the spindle checkpoint protein Mad1 and the mitotic kinase Nek2 PMID: 15611654
    16. tankyrase1 is a poly(ADP-ribose) polymerase with roles in telomere length control by the TRF1 component of the shelterin complex PMID: 17561506
    17. TRF1 regulates telomere length and function PMID: 18587156
    18. Thus, Pin1 is an essential regulator of TRF1 stability, telomere maintenance and ageing. PMID: 19060891
    19. TRF1, like TRF2, negatively regulates telomere length in vivo by controlling the action of the XPF nuclease at telomeres; in addition, TRF1 has a unique role in the mitotic spindle checkpoint. PMID: 19124610
    20. Gene deletion experiments showed that efficient duplication of telomeres requires the shelterin component TRF1. PMID: 19596237
    21. dysfunction of telomere repeat-binding factor 1 is sufficient to produce severe telomeric damage in the absence of telomere shortening, resulting in premature tissue degeneration and development of neoplastic lesions PMID: 19679647

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  • 亚细胞定位：
    Nucleus. Chromosome, telomere. Cytoplasm, cytoskeleton, spindle.
  • 数据库链接：

    KEGG: mmu:21749

    STRING: 10090.ENSMUSP00000027057

    UniGene: Mm.4306