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  4. Recombinant Mouse Syntaxin-binding protein 3 (Stxbp3)

Recombinant Mouse Syntaxin-binding protein 3 (Stxbp3)

  • 中文名称：
    小鼠Stxbp3重组蛋白
  • 货号：
    CSB-YP713891MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Stxbp3重组蛋白
  • 货号：
    CSB-EP713891MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Stxbp3重组蛋白
  • 货号：
    CSB-EP713891MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Stxbp3重组蛋白
  • 货号：
    CSB-BP713891MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Stxbp3重组蛋白
  • 货号：
    CSB-MP713891MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Stxbp3
  • Uniprot No.：
  • 别名：
    Stxbp3; Stxbp3a; Unc18c; Syntaxin-binding protein 3; MUNC-18-3; Mammalian homolog of Unc-18c; Munc-18c; Protein unc-18 homolog 3; Unc18-3; Protein unc-18 homolog C; Unc-18C
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-592
  • 氨基酸序列
    MAPPVSERGL KSVVWRKIKT AVFDDCRKEG EWKIMLLDEF TTKLLSSCCK MTDLLEEGIT VIENIYKNRE PVRQMKALYF ISPTPKSVDC FLRDFGSKSE KKYKAAYIYF TDFCPDSLFN KIKASCSKSI RRCKEINISF IPQESQVYTL DVPDAFYYCY SPDPSNASRK EVVMEAMAEQ IVTVCATLDE NPGVRYKSKP LDNASKLAQL VEKKLEDYYK IDEKGLIKGK TQSQLLIIDR GFDPVSTVLH ELTFQAMAYD LLPIENDTYK YKTDGKEKEA VLEEDDDLWV RVRHRHIAVV LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ISKQVVHLNL AEDCMNKFKL NIEKLCKTEQ DLALGTDAEG QRVKDSMLVL LPVLLNKNHD NCDKIRAVLL YIFGINGTTE ENLDRLIHNV KIEDDSDMIR NWSHLGVPIV PPSQQAKPLR KDRSAEETFQ LSRWTPFIKD IMEDAIDNRL DSKEWPYCSR CPAVWNGSGA VSARQKPRTN YLELDRKNGS RLIIFVIGGI TYSEMRCAYE VSQAHKSCEV IIGSTHILTP RKLLDDIKML NKSKDKVSFK DE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.
  • 基因功能参考文献：
    1. Investigated the use of Escherichia coli as an expression host for Munc18c. Three N-terminal His-tagged constructs were engineered, two with a tobacco etch virus (TEV) or thrombin protease cleavage site to enable removal of the fusion tag. PMID: 24391775
    2. PTP1B is the first known tyrosine phosphatase for Munc18c and a regulator of its phosphorylation and function in adipocytes. PMID: 23937695
    3. Ectopic expression of syntaxin3 affects behaviors of B16 melanoma by controlling actin dynamics. PMID: 23546178
    4. The Using 3T3-L1 adipocytes subjected to small interfering ribonucleic acid reduction of Munc18c as a model of impaired insulin-stimulated GLUT4 vesicle exocytosis. PMID: 21444687
    5. our data suggest that the mechanism by which glucosamine inhibits insulin-stimulated GLUT4 translocation involves modification of Munc18c. PMID: 12527361
    6. balance, more than absolute abundance, of Munc18c and Syn4 proteins directly affects whole-body glucose homeostasis through alterations in insulin secretion and insulin action. PMID: 12882905
    7. To define the molecular machinery required for platelet exocytosis, platelets from Munc18c heterozygous knockout mice were analyzed. These platelets show a decrease in Munc18c but no apparent reduction in other secretory machinery components PMID: 15467915
    8. Glucose intolerance and insulin secretion in knockout mice heterozygous for Munc18c are reported. PMID: 15734838
    9. Munc18c(L) gene is closely related to syntaxin-binding protein, Munc18c.It comprises 2 exons separated by a 600bp intron with non-consensus donor & acceptor sites.Exons 1 & 2 of Munc18c(L) overlap with exons 1 through half of 9 of Munc18c gene. PMID: 16036223
    10. novel and conserved mechanism for the dissociation of Munc18c-Syntaxin 4 complexes in a stimulus-dependent manner to facilitate the increase in Syntaxin 4-VAMP2 association and to promote vesicle/granule fusion PMID: 16638745
    11. support a sequential two-state model for SM/soluble NSF attachment protein receptors binding involving an initial interaction via the Syntaxins N-peptide PMID: 17517664
    12. We conclude, therefore, that Munc18c is not rate-limiting for cardiac substrate uptake, neither under basal conditions nor when maximally stimulated metabolically. PMID: 19009238
    13. Essential positive role for Munc18c in second-phase glucose-stimulated insulin secretion. PMID: 19188424

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  • 亚细胞定位：
    Cytoplasm, cytosol. Cell membrane.
  • 蛋白家族：
    STXBP/unc-18/SEC1 family
  • 组织特异性：
    Ubiquitously expressed in all tissues tested.
  • 数据库链接：

    KEGG: mmu:20912

    STRING: 10090.ENSMUSP00000099681

    UniGene: Mm.316894