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  4. Recombinant Mouse Spermine oxidase (Smox)

Recombinant Mouse Spermine oxidase (Smox)

  • 中文名称：
    小鼠Smox重组蛋白
  • 货号：
    CSB-BP021844MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Smox重组蛋白
  • 货号：
    CSB-EP021844MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Smox重组蛋白
  • 货号：
    CSB-MP021844MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Name:Smox Synonyms:Smo
  • Uniprot No.：
  • 别名：
    Smox; SmoSpermine oxidase; EC 1.5.3.16; Polyamine oxidase 1; PAO-1; PAOh1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-555
  • 氨基酸序列
    MQSCESSGDS ADDPLSRGLR RRGQPRVVVI GAGLAGLAAA RALLEQGFTD VTVLEASSHI GGRVQSVRLG DTTFELGATW IHGSHGNPIY QLAEANGLLE ETTDGERSVG RISLYSKNGV ACYLTNRGCR IPKDVVEEFS DLYNEVYNMT QEFFRHGKPV NAESQNSVGV FTREKVRNRI RDDPDDTEAT KRLKLAMIQQ YLKVESCESS SHSIDEVSLS AFGEWTEIPG AHHIIPSGFM RVVELLAEGI PPHVIQLGKP VRCIHWDQAS AHPRGPEIEP RGEGDHNHDT GEGGQSGENP QQGRWDEDEP WPVVVECEDC EVIPADHVIV TVSLGVLKRQ YTSFFRPCLP TEKVAAIHRL GIGTTDKIFL EFEEPFWGPE CNSLQFVWED EAESCTLTYP PELWYRKICG FDVLYPPERY GHVLSGWICG EEALVMERCD DEAVAEICTE MLRQFTGNPN IPKPRRILRS AWGSNPYFRG SYSYTQVGSS GADVEKLAKP LPYTESSKTA PMQVLFSGEA THRKYYSTTH GALLSGQREA ARLIEMYRDL FQQGP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.
  • 基因功能参考文献：
    1. Novel evidences of the complex and critical functions carried out by spermine oxidase and spermine in the mammalian brain. PMID: 23840306
    2. Spermine oxidase is an important regulator of muscle gene expression and fiber size. PMID: 25406264
    3. Data (including data from studies using recombinant mutant proteins) suggest that Glu216-Ser218 is a hot spot responsible for Smox substrate specificity; Smox with mutation E216L/S218A exhibits specificity for N'-acetylspermine rather than spermine. PMID: 24854736
    4. ROS overexposure by mSMOX delivers an earlier adaptive response to radiation PMID: 23857253
    5. Spermine oxidase mediates the gastric cancer risk associated with Helicobacter pylori CagA. PMID: 21839041
    6. Spermine oxidase binds spermine in a similar conformation as that observed in the yeast polyamine oxidase FMS1-spermine complex and demonstrate a major role for residues His82 and Lys367 in substrate binding and catalysis. PMID: 20839014
    7. expression and characterization of mouse spermine oxidase PMID: 12458219
    8. we identified a second domain that is necessary for nuclear localization of the spermine oxidase splice variant PMID: 15955064

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  • 亚细胞定位：
    Cytoplasm.; [Isoform 2]: Cytoplasm. Nucleus.
  • 蛋白家族：
    Flavin monoamine oxidase family
  • 组织特异性：
    Widely expressed. Isoform 1 and isoform 2 are expressed at higher level in brain and skeletal muscle. Isoform 7 is found in brain and spleen, isoform 10 is widely expressed but found at lower level in heart, kidney, liver and lung.
  • 数据库链接：

    KEGG: mmu:228608

    UniGene: Mm.136586