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  4. Recombinant Mouse Serum response factor (Srf)

Recombinant Mouse Serum response factor (Srf)

  • 中文名称：
    小鼠Srf重组蛋白
  • 货号：
    CSB-EP881354MO
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Srf; Serum response factor; SRF
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    67.2kDa
  • 表达区域：
    1-504aa
  • 氨基酸序列
    MLPSQAGAAAALGRGSALGGNLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGQSRLEREAAAAAAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSEMELGVVVGGPEAAAAAAGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATGFEEPDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPSTSTTMQVSSGPSFPITNYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPGGAVAQQVPVQAIHVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPTSGLADGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTAPSGTVQIPVSAVQLHQMAVIGQQAGSSSNLTELQVVNLDATHSTKSE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
  • 基因功能参考文献：
    1. The results demonstrate an unexpected function of Srf via a mechanism by which extracellular stimuli actively destabilize cell identity and suggest Srf involvement in a wide range of diseases. PMID: 29643333
    2. Forebrain-specific serum response factor deletion decreased acute stress-mediated immediate early gene induction. PMID: 27914009
    3. this study uncovered an SRF contribution to several processes of epileptogenesis in the pilocarpine model. PMID: 28716058
    4. these data indicate that Emerin, a conserved nuclear lamina protein, couples extracellular matrix mechanics and SRF-Mkl1-dependent transcription. PMID: 28576971
    5. findings reveal for the first time that SM22 is expressed in the nucleus in addition to the cytoplasm of VSMCs to regulate the transcription of Nik and its downstream proinflammatory NF-kB signal pathways as a modulator of SRF during vascular inflammation PMID: 29284006
    6. Cigarette smoke is one of the major causes for COPD pathogenesis, inducing COPD-associated skeletal muscle atrophy which is closely related to decreasing SRF nucleic translocation, and down-regulation of SRF target genes involved in muscle growth and nutrition. PMID: 28260872
    7. Synergy between TAZ and SRF in regulating smooth muscle gene activation was observed in primary aortic vascular smooth muscle cells. This novel signalling pathway links TGFbeta signalling to induction of smooth muscle genes through a mechanism involving regulation of TAZ and SRF proteins. PMID: 28342289
    8. Modifications at most transcription start sites are dependent on the TCF family of ERKregulated SRF cofactors. PMID: 28286024
    9. Blood pressure-associated polymorphism controls ARHGAP42 expression via serum response factor DNA binding PMID: 28112683
    10. regulates smooth muscle contractility via myotonic dystrophy protein kinases and L-type calcium channels PMID: 28152551
    11. knockdown of MKL1 induces a significant increase in the transcriptional activity of PPARgamma target genes and MKL1 interacts with PPARg, suggesting that SRF and MKL1 independently inhibit brown adipogenesis and that MKL1 exerts its effect mainly by modulating PPARgamma activity PMID: 28125644
    12. Our results elucidate the specific role of the transcription factors CREB, SRF, and MEF2 in the depression and potentiation components of ODP in vivo, therefore better informing future attempts to find therapeutic targets for diseases where activity-dependent plasticity is disrupted. PMID: 28607167
    13. The actin cytoskeleton regulatory pathway, MAPK and dilated cardiomyopathy signaling pathways, as well as CFL1 and ITGB6 genes, may be regulated by Srf to serve important roles in the progression of corneal disease. PMID: 27922676
    14. The inducible and conditional deletion of SRF in the adult mouse hippocampus increases the epileptic phenotype in the kainic acid model of epilepsy. There was a robust decrease in activity-induced gene transcription in SRF knockout mice. SRF target genes are associated with synaptic plasticity and epilepsy. PMID: 25636686
    15. the controlled and timely activation of SRF is essential for the coordinated growth of neuronal processes. PMID: 26638868
    16. Increased serum response factor activity provokes podocytes' epithelial-mesenchymal transition and dysfunction in diabetic nephropathy. PMID: 26408645
    17. A role for SRF in the transcriptional regulation of DUSP5 PMID: 26691724
    18. Exploration of the molecular causes of enhanced cardiac hypertrophy revealed significant activation of beta-catenin/GSK-3beta signaling, whereas MAPK and MKL1/serum-response factor pathways were inhibited. PMID: 26719331
    19. these findings reveal a key role of the SRF/CTGF/miR-133a axis in the regulation of cardiac fibrosis PMID: 26440278
    20. Study uncovered important roles of neurons and neuronally expressed SRF in multiple sclerosis associated de- and remyelination PMID: 25639799
    21. These data support a central role of the SRF/MRTF pathway in the pathobiology of lung fibrosis. PMID: 25681733
    22. the actin, myocardin-related transcription factors and serum response factor (actin-Mrtf-Srf) pathway is specifically downregulated in the muscle atrophy that is induced through disuse in mice. PMID: 25344251
    23. These results identify SRF and its MRTF cofactors as major transcriptional regulators of endothelial cell junctional stability, guaranteeing physiological functions of the cerebral microvasculature. PMID: 26221020
    24. Silencing Malat1 inhibits myocyte differentiation and decreases Srf at RNA and protein levels. Srf silencing decreases Malat1 expression. Malat1 contains an miR-133 functional target site, and the interplay between Malat1 and Srf is miR-133 dependent. PMID: 25868726
    25. SRF cooperated with MEF2 to sustain the expression of LMOD3 and other components of the contractile apparatus, thereby establishing a regulatory circuit to maintain skeletal muscle function. PMID: 25774500
    26. results indicate that Foxf2 signaling in smooth muscle cells is essential for intestinal development and serum response factor signaling PMID: 25631042
    27. Identified a set of direct SRF target genes acting in posterior nascent mesoderm which are enriched for transcripts associated with migratory function. We further show that cell migration is impaired in Srf mutant embryos. PMID: 25020278
    28. SRF-VP16iHep murine hepatocellular carcinoma reveal convergent Ras/MAPK and Rho/actin signaling as a highly oncogenic driver mechanism for hepatocarcinogenesis PMID: 25266280
    29. leading to a PDGF-responsive SRF-driven transcriptional program in the midface PMID: 25453829
    30. Increased SRF negatively affects transcription of SRF gene targets. PMID: 24550211
    31. Data show that the binding of WIP to actin controls the actin dynamics MRTFA-SRF-Focal adhesion assembly signaling cascade. PMID: 24797074
    32. MKL1/2 and ELK4 co-regulate distinct serum response factor (SRF) transcription programs in macrophages. PMID: 24758171
    33. Data indicate that despite normal neutrophil numbers, neutrophil function is severely impaired in serum response factor (SRF) knockout (KO) neutrophils. PMID: 24574460
    34. SRF-dependent miR-210 expression may operate as a novel silencer of the Shh signaling pathway. PMID: 23818299
    35. Our findings reveal a novel phosphorylation and activation of SRF by GSK-3 that is critical for SRF-dependent axon growth in mammalian central neurons. PMID: 24623780
    36. Serum response factor indirectly regulates type I interferon-signaling in macrophages. PMID: 23705899
    37. Data indicate that interferon regulatory factor 8 (IRF8) inhibited smooth muscle cells (SMCs) marker gene expression through regulating serum response factor (SRF) transactivation in a myocardin-dependent manner. PMID: 24248596
    38. sustained Akt1-induced alphaSMA synthesis markedly decreased upon RNA silencing of SRF and myocardin. PMID: 24106278
    39. Overexpression of transcription factors MYOCD and SRF alone or in conjunction with Mesp1 and SMARCD3 enhanced the basal but necessary cardio-inducing effect of the previously reported GATA4, TBX5, and MEF2C. PMID: 23704920
    40. Foxf1 also directly binds to serum response factor (SRF) and myocardin-related transcription factors (MRTFs). PMID: 23946491
    41. these results strongly support a role for -mediated alternative splicing in the regulation of contractile gene expression, achieved in part through modulating the activity of SRF, a key cardiac transcription factor. PMID: 23437181
    42. Lamin A/C and emerin regulate MKL1-SRF activity by modulating actin dynamics PMID: 23644458
    43. SRF is selectively required for endothelial filopodia formation and cell contractility during sprouting angiogenesis, but seems dispensable for vascular remodeling PMID: 23674601
    44. angiogenic homeostasis is ensured by differential stage-specific functions of SRF target gene products in the developing versus the mature retinal vasculature PMID: 23563308
    45. Serum response factor controls transcriptional network regulating epidermal function and hair follicle morphogenesis. PMID: 23151848
    46. Activated mDia promoted rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity. PMID: 23558171
    47. SRF-related decreases in vasomotor tone and cell-matrix attachment increase arterial elasticity in large arteries. PMID: 23426017
    48. Data suggest that in mouse liver SRF is regulated via dramatic diurnal changes of actin dynamics, leading to the rhythmic translocation of the SRF coactivator Myocardin-related transcription factor-B (MRTF-B) into the nucleus. PMID: 23374345
    49. SRF has a major part to play in the control of local blood flow via its central role in pressure-induced myogenic tone in resistance arteries. PMID: 23264443
    50. SRF plays a key role in the modulation of cardiac fibrosis through repression of cardiomyocyte CTGF expression in a paracrine fashion. PMID: 22563064

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: mmu:20807

    STRING: 10090.ENSMUSP00000015749

    UniGene: Mm.45044