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  4. Recombinant Mouse Serine/threonine-protein kinase Sgk1 (Sgk1)

Recombinant Mouse Serine/threonine-protein kinase Sgk1 (Sgk1)

  • 中文名称：
    小鼠Sgk1重组蛋白
  • 货号：
    CSB-YP893904MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Sgk1重组蛋白
  • 货号：
    CSB-EP893904MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Sgk1重组蛋白
  • 货号：
    CSB-EP893904MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Sgk1重组蛋白
  • 货号：
    CSB-BP893904MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Sgk1重组蛋白
  • 货号：
    CSB-MP893904MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Sgk1; Sgk; Serine/threonine-protein kinase Sgk1; EC 2.7.11.1; Serum/glucocorticoid-regulated kinase 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-431
  • 氨基酸序列
    MTVKAEAARS TLTYSRMRGM VAILIAFMKQ RRMGLNDFIQ KIASNTYACK HAEVQSILKM SHPQEPELMN ANPSPPPSPS QQINLGPSSN PHAKPSDFHF LKVIGKGSFG KVLLARHKAE EVFYAVKVLQ KKAILKKKEE KHIMSERNVL LKNVKHPFLV GLHFSFQTAD KLYFVLDYIN GGELFYHLQR ERCFLEPRAR FYAAEIASAL GYLHSLNIVY RDLKPENILL DSQGHIVLTD FGLCKENIEH NGTTSTFCGT PEYLAPEVLH KQPYDRTVDW WCLGAVLYEM LYGLPPFYSR NTAEMYDNIL NKPLQLKPNI TNSARHLLEG LLQKDRTKRL GAKDDFMEIK SHIFFSLINW DDLINKKITP PFNPNVSGPS DLRHFDPEFT EEPVPSSIGR SPDSILVTAS VKEAAEAFLG FSYAPPVDSF L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response. Contributes to regulation of renal Na(+) retention, renal K(+) elimination, salt appetite, gastric acid secretion, intestinal Na(+)/H(+) exchange and nutrient transport, insulin-dependent salt sensitivity of blood pressure, salt sensitivity of peripheral glucose uptake, cardiac repolarization and memory consolidation. Up-regulates Na(+) channels: SCNN1A/ENAC, SCN5A and ASIC1/ACCN2, K(+) channels: KCNJ1/ROMK1, KCNA1-5, KCNQ1-5 and KCNE1, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channels: BSND, CLCN2 and CFTR, glutamate transporters: SLC1A3/EAAT1, SLC1A2 /EAAT2, SLC1A1/EAAT3, SLC1A6/EAAT4 and SLC1A7/EAAT5, amino acid transporters: SLC1A5/ASCT2, SLC38A1/SN1 and SLC6A19, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, glutamate receptor: GRIK2/GLUR6. Up-regulates carriers: SLC9A3/NHE3, SLC12A1/NKCC2, SLC12A3/NCC, SLC5A3/SMIT, SLC2A1/GLUT1, SLC5A1/SGLT1 and SLC15A2/PEPT2. Regulates enzymes: GSK3A/B, PMM2 and Na(+)/K(+) ATPase, and transcription factors: CTNNB1 and nuclear factor NF-kappa-B. Stimulates sodium transport into epithelial cells by enhancing the stability and expression of SCNN1A/ENAC. This is achieved by phosphorylating the NEDD4L ubiquitin E3 ligase, promoting its interaction with 14-3-3 proteins, thereby preventing it from binding to SCNN1A/ENAC and targeting it for degradation. Regulates store-operated Ca(+2) entry (SOCE) by stimulating ORAI1 and STIM1. Regulates KCNJ1/ROMK1 directly via its phosphorylation or indirectly via increased interaction with SLC9A3R2/NHERF2. Phosphorylates MDM2 and activates MDM2-dependent ubiquitination of p53/TP53. Phosphorylates MAPT/TAU and mediates microtubule depolymerization and neurite formation in hippocampal neurons. Phosphorylates SLC2A4/GLUT4 and up-regulates its activity. Phosphorylates APBB1/FE65 and promotes its localization to the nucleus. Phosphorylates MAPK1/ERK2 and activates it by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Phosphorylates FBXW7 and plays an inhibitory role in the NOTCH1 signaling. Phosphorylates FOXO1 resulting in its relocalization from the nucleus to the cytoplasm. Phosphorylates FOXO3, promoting its exit from the nucleus and interference with FOXO3-dependent transcription. Phosphorylates BRAF and MAP3K3/MEKK3 and inhibits their activity. Phosphorylates SLC9A3/NHE3 in response to dexamethasone, resulting in its activation and increased localization at the cell membrane. Phosphorylates CREB1. Necessary for vascular remodeling during angiogenesis.
  • 基因功能参考文献：
    1. SGK1 influences migration of VSMC by transcriptional regulation of Ca2+ channel Orai1 expression, an effect requiring SGK1-dependent activation of transcription factor NF-B after arterial injury. PMID: 28203685
    2. results suggest that SGK1 may play a critical role in the inflammatory cascade of cardiac fibroblasts triggered by mechanical stretch; SGK1 could be used as a potential target for treatment of cardiac fibrosis and heart failure PMID: 29243066
    3. Decreased expression of SGK1 may play a critical role in increasing the expression of alpha-syn, which is related with dopaminergic cell death in the Substantia nigra of chronic MPTP-induced Parkinsonism mice and in SH-SY5Y cells. PMID: 29604467
    4. High SGK1 expression is associated with anxiety/depression. PMID: 27502757
    5. Experiments showed a dynamic change of immune inflammation, oxidative stress and p38MAPK-SGK1 pathway involvement in EAE demonstrating that p38MAPK-SGK1 pathway and oxidative stress contribute to the demyelination in central nerve system caused by Th17 inflammatory responses in a synergistic way. PMID: 28467798
    6. SGK1 was found to be essential for proliferation and survival of thyroid cancer cells harboring PI3K-activating mutations. PMID: 29055016
    7. Findings illustrate how cancer cells utilize a chromatin remodeling factor to engage a core survival pathway to support its cancerous phenotypes, and reveal new facets of MTA1-SGK1 axis by a physiologic signal in cancer progression. PMID: 28504714
    8. Altered K balance in animals lacking SGK1 may reflect defects in epithelial Na channel -independent K excretion PMID: 27413200
    9. our data suggest that renal tubular SGK1 is important in the regulation of K(+) excretion via the control of NEDD4-2, WNK1, and ENaC PMID: 27009335
    10. SGK1 is similarly involved in the regulation of cell volume and cell fluid transport. PMID: 27861175
    11. Mineralocorticoid receptor deficiency suppresses migration and proliferation of macrophages and leads to less vascular smooth muscle cell activation. At the molecular level, MR deficiency suppresses macrophage inflammatory response via SGK1-AP1/NF-kappaB pathways. PMID: 26966277
    12. Akt3 constitutively suppresses macropinocytosis in macrophages through a novel WNK1/SGK1/Cdc42 pathway. PMID: 28389565
    13. SGK1 and SGK3 are expressed in multiple microglial cell lines. SGK1 and SGK3 may play an important role in regulating microglial viability and inflammatory responses. PMID: 27457803
    14. The data indicate that the balanced activities of two related serine/threonine kinases, AKT and SGK1, critically govern the embryo implantation process. PMID: 27825168
    15. LEFTY2 regulates the expression and activity of ENaC in endometrial epithelial cells via SGK1. PMID: 27606670
    16. high levels of resistin observed during obesity may activate SGK1 in the vasculature and contribute to the development of obesity-related vascular disease PMID: 26833885
    17. High salt diet may deteriorate lupus nephritis through SGK1 pathway. PMID: 26474695
    18. Study shows that SGK1 impairs nuclear abundance of p27 and suggests that phosphorylation of p27T197 by SGK1 is required for cardiac hypertrophy. PMID: 26344141
    19. SGK1 appears to be an important enzyme in the process of fibrotic remodeling and subsequent weakness of dystrophin-deficient mouse muscle PMID: 25394886
    20. The characterisation of SGK1 functions in obesity and immunity may help identify potential therapeutic targets in the treatment of obesity. PMID: 25809829
    21. Identify SGK1 as a novel powerful regulator of platelet dense granule biogenesis, platelet secretion and thrombus formation. SGK1 is at least partially effective because it regulates transcription of Rab27b in megakaryocytes. PMID: 25944668
    22. SGK1 plays a pivotal role in vascular inflammation during atherogenesis. SGK1 participates in the regulation of monocyte/macrophage migration and MMP-9 transcription via regulation of nuclear factor-kappaB. PMID: 25614279
    23. SGK1 inhibits intestinal epithelial cell apoptosis and promotes proliferation via the MEK/ERK/p53 pathway in colitis. PMID: 26034353
    24. measured gene expression of Fos, Per1 and Sgk1 45 min after exposure to brief cold swim stress in male and female mice; Sgk1 was insensitive to handling, and increased in response to stress similarly in males and females PMID: 25459888
    25. 17beta-estradiol attenuates LPS-induced acute lung injury via PI3K/Akt/SGK1 mediated up-regulation of Scnn1a. PMID: 25551628
    26. These findings have identified an anti-inflammatory function of SGK1, elucidated the underlying intracellular mechanisms PMID: 25993992
    27. Sgk1 regulates Dsg1c expression levels in oligodendrocytes in the mouse corpus callosum after chronic stress exposure. PMID: 26043694
    28. Macrophages that expressed SGK1 were able to promote pulmonary arterial smooth muscle cells proliferation, which was lost with SGK1-deficient mice. PMID: 24825325
    29. SGK1 and NDRG1 participate in the regulation of NF-kappaB signaling in and maturation of DCs PMID: 25200670
    30. SGK1 inhibits ERK1/2 (extracellular-signal-regulated kinase 1/2) activity in liver and Ad-shERK1/2-mediated inhibition of ERK1/2 reverses the attenuated insulin sensitivity in Ad-shSGK1 mice PMID: 25222560
    31. Repeated, but not acute, morphine or cocaine administration increases SGK1 gene expression and activity in the ventral tegmental area. This increase may play a role in drug-dependent behaviors. PMID: 25099208
    32. mTORC1, in coordination with mTORC2, controls cell proliferation by regulating FoxO3a gene expression and SGK1-mediated phosphorylation of FoxO3a at Ser314 PMID: 24558442
    33. SGK-dependent phosphorylation of glycogen synthase kinase GSK-3 contributes to the pro-fibrotic signaling leading to renal tissue fibrosis in obstructive nephropathy PMID: 24685987
    34. Pendrin is expressed in platelets and is presumably regulated by SGK1 and mineralocorticoids. PMID: 24429827
    35. This study underscores the importance of SGK1 signalling in cardiac neo-angiogenesis and wound healing after an ischemic insult in vivo. PMID: 24265802
    36. promotes T helper type 2 differentiation after activation by mTORC2 PMID: 24705297
    37. adaptive molecular responses of glucocorticoid-responsive gene expression and SGK1 in prefrontal cortex may contribute to mechanisms underlying behavioral responses to chronic ethanol exposure PMID: 23991167
    38. stress-induced Sgk-1 localizes to the mitochondria, which permits access to mitochondrial interacting proteins and substrates, such as IF-1 and the F1F0-ATPase PMID: 23402912
    39. SGK1 has a critical role in the induction of pathogenic TH17 cells and provide a molecular insight into a mechanism by which an environmental factor such as a high salt diet triggers TH17 development and promotes tissue inflammation PMID: 23467085
    40. Renally expressed Sgk1 isoform, Sgk1_i3, stimulates ENaC activity when heterologously expressed in collecting duct cells. PMID: 23034940
    41. SGK1-dependent divalent calcium (Ca2+) ion-labelled entry into mast cells is a novel mechanism that regulates the cell actin cytoskeleton. PMID: 23015548
    42. Cardiac-specific SGK1 activation increased mortality, cardiac dysfunction, & ventricular arrhythmias via sodium channels, but protected from fibrosis & heart failure after stress. SGK1 binds directly to Nav1.5. PMID: 23019294
    43. SGK1 participates in the signaling of platelet migration PMID: 22759972
    44. SGK1 is a gamma-secretase regulator presumably effective through phosphorylation and degradation of NCT. PMID: 22590650
    45. SGK1 may play an important role in macrophage recruitment and M2 macrophage activation by activating the STAT3 pathway, which leads to angiotensin II-induced cardiac fibrosis. PMID: 22556335
    46. The impaired ability of Sgk1 knockout mice to retain Na+ increased significantly with a low-salt diet despite higher plasma aldosterone levels. On a low-Na+ diet, the Sgk1 knockout mice were also hyperkaliuric and lost body weight. PMID: 22301619
    47. According to the present observations, SGK1 is a major determinant of NHE1 activity in the failing heart. PMID: 22212557
    48. Data show that deregulated SGK1 activity in cycling endometrium interferes with embryo implantation, leading to infertility, or predisposes to pregnancy complications by rendering the feto-maternal interface vulnerable to oxidative damage. PMID: 22001908
    49. Data show that vitamin D treatment upregulates the expression of SGK1, which in turn enhances SGLT1 activity. PMID: 21735060
    50. Neural precursor cell-expressed developmentally down-regulated protein 4-2 (Nedd4-2) regulation by 14-3-3 protein binding at canonical serum and glucocorticoid kinase 1 (SGK1) phosphorylation sites. PMID: 21900244

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cell membrane. Mitochondrion.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family
  • 数据库链接：

    KEGG: mmu:20393

    STRING: 10090.ENSMUSP00000114074

    UniGene: Mm.28405