Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Serine protease inhibitor Kazal-type 1 (Spink1)

Recombinant Mouse Serine protease inhibitor Kazal-type 1 (Spink1)

In Stock
  • 中文名称：
    小鼠Spink1重组蛋白
  • 货号：
    CSB-YP362579MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Spink1
  • Uniprot No.：
  • 别名：
    Spink1; Spink3; Serine protease inhibitor Kazal-type 1; P12; Prostatic secretory glycoprotein; Serine protease inhibitor Kazal-type 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    8.1kDa
  • 表达区域：
    24-80aa
  • 氨基酸序列
    AKVTGKEASCHDAVAGCPRIYDPVCGTDGITYANECVLCFENRKRIEPVLIRKGGPC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The recombinant mouse Spink1 protein is a fusion protein consists of the mouse Spink1 protein (24-80aa) partnered with the N-terminal 6xHis tag. It was produced in the yeast. This recombinant Spink1 protein's purity is greater than 90% determined by SDS-PAGE. After electrophoresis, there is a 12 kDa protein band presented on the gel.

    SPINK1 is a secreted protein known as a protease inhibitor of trypsin in the pancreas. However, emerging evidence shows its function in promoting cancer progression in various types of cancer. It was first discovered in bovine pancreas extracts. SPINK1 is also known as tumor-associated trypsin inhibitor (TATI), was further isolated from the urine of ovarian cancer patients by another research group. SPINK1 and TATI were later characterized as identical molecules. The human SPINK1 gene encodes an mRNA that is spliced into three transcript variants, which can be translated into a 79-amino acid peptide, including a 23-amino-acid signal peptide. Interestingly, a SPINK1 interaction with epidermal growth factor receptor (EGFR) in rat liver hepatocyte line of BRL-3A in vitro has been indicated. SPINK1 reprograms the expression profile of prostate cancer cells, leading to prominent epithelial–endothelial transition (EET), a phenotypic switch mediated by EGFR signaling. Presently, the interaction of SPINK1 has attracted attention for its potentially pivotal biological functions, especially in modulating cancer progression.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine protease inhibitor which exhibits anti-trypsin activity. In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.; In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.
  • 基因功能参考文献：
    1. It is a trypsin-specific inhibitor in the pancreas. PMID: 24521862
    2. IFN regulatory factor 2 (Irf2) has a regulatory role in trypsinogen5 gene transcription, which is resistant to a major endogenous trypsin inhibitor, Spink3 PMID: 22042864
    3. SPINK3 modulates sperm physiology through a downstream reduction of endogenous NO concentration and independently of SPINK3 trypsin inhibitory activity. PMID: 22228629
    4. uterine-gland-derived SPINK3, as a new paracrine modulator, might play an important role in embryo implantation through its influence on stromal decidualization in mice. PMID: 20623140
    5. Spink3 may act as a growth factor, leading to cell proliferation and regeneration of tissue structure. PMID: 20660988
    6. Results suggest that regulation of Prss1 and Spink3 expression is involved in the susceptibility to experimentally induced pancreatitis. PMID: 20157294
    7. Spink3 trypsin inhibitor is required for normal pancreatic development. Data indicate an important relationship between endogenous pancreatic trypsin and Spink3 and the development of pancreatitis. PMID: 20110462
    8. SPINK3 mRNA is upregulated in chronic pancreatitis. PMID: 15097871
    9. Spink3 may play important roles in proliferation and/or differentiation of various cell types during development. PMID: 18386042

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    In the genital tract, expressed only in male accessory glands including seminal vesicle, coagulating gland and prostate.
  • 数据库链接：

    KEGG: mmu:20730

    STRING: 10090.ENSMUSP00000025381

    UniGene: Mm.272