Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Regulator of G-protein signaling 7-binding protein (Rgs7bp)

Recombinant Mouse Regulator of G-protein signaling 7-binding protein (Rgs7bp)

  • 货号：
    CSB-YP806890MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP806890MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806890MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806890MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806890MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rgs7bp
  • Uniprot No.：
  • 别名：
    Rgs7bp; D13Bwg1146e; R7bp; Regulator of G-protein signaling 7-binding protein; R7 family-binding protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-257
  • 氨基酸序列
    MSSAPNGRKK RPSRSTRSSI FQISKPPLQS GDWERRGSGS ESAHKTQRAL DDCKMLVQEF NTQVALYREL VISIGDVSVS CPSLRAEMHK TRTKGCEMAR QAHQKLAAIS GPEDGEIHPE ICRLYIQLQC CLEMYTTEML KSICLLGSLQ FHRKGKEASG GAKNLDSKIE ENAETPALED SLSSPLESQQ QCWQVATDIE NTERDMREMK NLLSKLRETM PLPLKNQDDS SLLNLTPYPM VRRRKRRFFG LCCLVSS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulator of G protein-coupled receptor (GPCR) signaling. Regulatory subunit of the R7-Gbeta5 complexes that acts by controlling the subcellular location of the R7-Gbeta5 complexes. When palmitoylated, it targets the R7-Gbeta5 complexes to the plasma membrane, leading to inhibit G protein alpha subunits. When it is unpalmitoylated, the R7-Gbeta5 complexes undergo a nuclear/cytoplasmic shuttling. May also act by controlling the proteolytic stability of R7 proteins, probably by protecting them from degradation.
  • 基因功能参考文献：
    1. The results of this study indicated that R7bp is a key regulator of itch sensation and suggest the potential targeting of R7bp-dependent GTPase activating protein activity as a novel therapeutic strategy for pathological itch PMID: 28134655
    2. R7BP-dependent regulation of R7-RGS proteins shapes specific aspects of light-evoked and spontaneous activity of retinal ganglion cells in mature and developing retina. PMID: 24349243
    3. GIRK modulation occurs by channel assembly with R7-RGS/Gbeta5 complexes under allosteric control of R7 RGS-binding protein (R7BP). PMID: 23169654
    4. R7BP is a negative modulator of the analgesic and locomotor activating actions of morphine that contributes to the development of morphine tolerance. PMID: 22089315
    5. regulator of G-protein signalling 7 binding protein contributes significantly to the nuclear localization of endogenous G beta(5)/R7-RGS complex in brain PMID: 20100282
    6. These results indicate that dopamine signaling in the striatum is controlled by concerted interplay between two RGS proteins, RGS7 and RGS9-2, which are balanced by a common subunit, R7BP. PMID: 20043004
    7. All four R7 RGS proteins co-precipitate with R7BP from brain extracts and recombinant R7 proteins bind recombinant R7BP with high efficiency PMID: 15632198
    8. Review. R7BP transduces signals directly from receptors and G proteins at the plasma membrane to the nucleus, and this plasma membrane-nuclear shuttling is controlled by reversible palmitoylation of R7BP. PMID: 16046666
    9. R7BP controls expression of regulator of G-protein signaling (RGS)9-2 expression at the posttranslational level. PMID: 18094251
    10. R7-Gbeta5-R7BP complexes in the mouse could regulate signaling by modulatory Gi/o-coupled GPCRs in the developing and adult nervous systems. PMID: 18248908
    11. Gbeta5 and R7BP in striatum undergo remodeling upon changes in neuronal activity. PMID: 19332565

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus. Cytoplasm. Cell membrane; Lipid-anchor.
  • 蛋白家族：
    RGS7BP/RGS9BP family
  • 组织特异性：
    Specifically expressed in the central nervous system including the retina but not in other non-neuronal tissues (at protein level).
  • 数据库链接：

    KEGG: mmu:52882

    STRING: 10090.ENSMUSP00000066614

    UniGene: Mm.100348