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  4. Recombinant Mouse Ras-related protein Rab-8A (Rab8a)

Recombinant Mouse Ras-related protein Rab-8A (Rab8a)

  • 中文名称：
    小鼠Rab8a重组蛋白
  • 货号：
    CSB-YP019222MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rab8a重组蛋白
  • 货号：
    CSB-EP019222MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rab8a重组蛋白
  • 货号：
    CSB-EP019222MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rab8a重组蛋白
  • 货号：
    CSB-BP019222MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rab8a重组蛋白
  • 货号：
    CSB-MP019222MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rab8a; Mel; Rab8; Ras-related protein Rab-8A; Oncogene c-mel
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-204
  • 氨基酸序列
    MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI ELDGKRIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR NWIRNIEEHA SADVEKMILG NKCDVNDKRQ VSKERGEKLA LDYGIKFMET SAKANINVEN AFFTLARDIK AKMDKKLEGN SPQGSSHGVK ITVEQQKRTS FFRC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. May be involved in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. Involved in autophagy.
  • 基因功能参考文献：
    1. Disruption of Rab8a and Rab11a causes formation of basolateral microvilli in neonatal enteropathy. PMID: 28596241
    2. The small GTPase Rab8a regulates lipid uptake and storage in skeletal muscle. PMID: 28696211
    3. Rab8a and Rab11a Are Dispensable for Rhodopsin Transport in Mouse Photoreceptors PMID: 27529348
    4. With CRISPR/Cas9-mediated gene editing to stably knock out and recover Rab8a in macrophage cell lines, this study matches Akt signaling profiles with cytokine outputs, confirming that Rab8a is a novel regulator of the Akt/mammalian target of rapamycin (mTOR) pathway downstream of multiple Toll-like Receptors. PMID: 28130450
    5. these results indicate an indispensable role for Rab8A in insulin-regulated GLUT4 trafficking in C2C12 cells. PMID: 28079283
    6. Results highlight a novel role of Rab8, downstream of IFT20, in the pathway that regulates T cell receptor (TCR) recycling, through recruiting VAMP-3 and promoting the fusion with the synaptic membrane of endosomes carrying TCR cargoes. PMID: 26587735
    7. Data demonstrate that EHBP1L1 links Rab8 and the Bin1-dynamin complex, which generates membrane curvature and excises the vesicle at the endocytic recycling compartment for apical transport. PMID: 26833786
    8. The small GTPase Rab8 interacts with VAMP-3 to regulate the delivery of recycling T-cell receptors to the immune synapse. PMID: 26034069
    9. Rab8a thus controls Wnt delivery in producing cells and is crucial for Paneth cell maturation. PMID: 26015543
    10. Rab8a acts as an activator of Fsp27-mediated LD fusion and growth. PMID: 25158853
    11. Simultaneous loss of Rab8a and Rab8b has little effect on ciliogenesis, whereas additional loss of Rab10 greatly affects ciliogenesis. PMID: 24213529
    12. acidosis-induced NBCe1-A, but not NBCe1-B/C, trafficking is mediated by Rab8a. PMID: 23583738
    13. AS160 and Rab8a regulate membrane recruitment of CD36. PMID: 22315395
    14. Cdc42 and Rab8a are critical for intestinal stem cell division, survival, and differentiation in mice PMID: 22354172
    15. Rab8a colocalizes with IL-1b and LC3 and controls IL-1b secretion PMID: 22068051
    16. Rab13 and Rab8A are Rab-GTPases activated by insulin and downstream of AS160 they regulate traffic of GLUT4 vesicles. PMID: 21041651
    17. These results suggest that the interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions. PMID: 18094055
    18. GLUT4 vesicle recruitment and fusion are differentially regulated by Rac, AS160, and Rab8A in muscle cells. PMID: 18650435
    19. Ahi1 is required for Rab8a localization, ciliogenesis and vesicle trafficking. PMID: 19625297

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Recycling endosome membrane. Cell projection, cilium. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, cilium basal body. Midbody. Cytoplasm.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 数据库链接：

    KEGG: mmu:17274

    STRING: 10090.ENSMUSP00000003121

    UniGene: Mm.162811