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  4. Recombinant Mouse Rac GTPase-activating protein 1 (Racgap1)

Recombinant Mouse Rac GTPase-activating protein 1 (Racgap1)

  • 中文名称：
    小鼠Racgap1重组蛋白
  • 货号：
    CSB-YP893438MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Racgap1重组蛋白
  • 货号：
    CSB-EP893438MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Racgap1重组蛋白
  • 货号：
    CSB-EP893438MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Racgap1重组蛋白
  • 货号：
    CSB-BP893438MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Racgap1重组蛋白
  • 货号：
    CSB-MP893438MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Racgap1
  • Uniprot No.：
  • 别名：
    Racgap1; Mgcracgap; Rac GTPase-activating protein 1; Male germ cell RacGap; MgcRacGAP
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-628
  • 氨基酸序列
    MDTTMVNLWT LFEQLVRRME IINEGNESIE FIQVVKDFED FRKKYQRTNQ ELEKFKDLLL KAETGRSALD VKLKHARNQV DVEIKRRQRA EAECAKLEQQ IQLIRDILMC DTSGSIQLSE EQKSALAFLN RGQASSGHAG NNRLSTIDES GSILSDISFD KTDESLDWDS SLVKNFKMKK REKRRSNSRQ FIDGPPGPVK KTCSIGSTVD QANESIVAKT TVTVPSDGGP IEAVSTIETL PSWTRSRGKS GPLQPVNSDS ALNSRPLEPR TDTDNLGTPQ NTGGMRLHDF VSKTVIKPES CVPCGKRIKF GKLSLKCRDC RLVSHPECRD RCPLPCIPPL VGTPVKIGEG MLADFVSQAS PMIPAIVVSC VNEIEQRGLT EAGLYRISGC DRTVKELKEK FLKVKTVPLL SKVDDIHVIC SLLKDFLRNL KEPLLTFWLS KAFMEAAEIT DEDNSTAAMY QAVSELPQAN RDTLAFLMIH LQRVSQSPDT KMDIANLAKV FGPTIVAHTV PNPDPVTMFQ DIKRQLKVVE RLLSLPLEYW NQFMMVDQEN IDSQRGNGNS TPRTPDVKVS LLGPVTTPEF QLVKTPLSSS LSQRLYNLSK STPRFGNKSK SATNLGQQGK FFPAPYLK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
  • 基因功能参考文献：
    1. Deletion of MgcRacGAP in the male germ cells impairs spermatogenesis and causes male sterility in the mouse. PMID: 24355749
    2. APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PMID: 23696789
    3. several Rho family small GTPases activate PI3K by an indirect cooperative positive feedback that required a combination of Rac, CDC42, and RhoG small GTPase activities PMID: 22683270
    4. These results indicate that MgcRacGAP regulates cytokinesis and cellular differentiation as a regulator of Rho family of GTPase and suggest that this process is controlled by some serine/threonine kinases. PMID: 11942621
    5. alpha5 is required for proliferation and polarity of basal epithelial cells; the interaction between laminin-10/11-integrin alpha6beta4 and the PI3-kinase-Cdc42/Rac pathways may play a role in determining the size and shape of tooth germ PMID: 16365040
    6. Gab2 via Shp-2 is critical for transmitting signals from Kit Tyr(567) to activate the Rac/JNK pathway controlling mast cell proliferation PMID: 16873377
    7. Data demonstrate that normal cytokinesis in B lymphocytes requires the GAP and NH2 terminal domains but not GAP activity of mgcRacGAP. PMID: 16959247
    8. Rac became activated within 2 min after peripheral membrane extensions adhered to new ECM islands, and this activation wave propagated outward in an oriented manner as the cells spread from island to island PMID: 18180334
    9. mgcRacGAP is now shown to play a indispensable role in the development of hematopoietic cells in vivo. PMID: 18541143
    10. This study demonstrated that loss of Rac1 attenuates synapse structure and function in vivo, which ultimately leads to significant defects in spatial learning and working/episodic-like memory. PMID: 19394428

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasmic vesicle, secretory vesicle, acrosome. Cleavage furrow. Midbody, Midbody ring. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 组织特异性：
    Highly expressed in testis, thymus and spleen and weakly expressed in brain, heart, skeletal muscle and kidney. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Not detected in adult liver. Al
  • 数据库链接：

    KEGG: mmu:26934

    STRING: 10090.ENSMUSP00000023756

    UniGene: Mm.273804