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  4. Recombinant Mouse Rabphilin-3A (Rph3a)

Recombinant Mouse Rabphilin-3A (Rph3a)

  • 中文名称：
    小鼠Rph3a重组蛋白
  • 货号：
    CSB-YP020102MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rph3a重组蛋白
  • 货号：
    CSB-EP020102MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rph3a重组蛋白
  • 货号：
    CSB-EP020102MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rph3a重组蛋白
  • 货号：
    CSB-BP020102MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rph3a重组蛋白
  • 货号：
    CSB-MP020102MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rph3a
  • Uniprot No.：
  • 别名：
    Rph3a; Rabphilin-3A; Exophilin-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-681
  • 氨基酸序列
    MTDTVVNRWM YPGDGPLQSN DKEQLQAGWS VHPGAQTDRQ RKQEELTDEE KEIINRVIAR AEKMEAMEQE RIGRLVDRLE TMRKNVAGDG VNRCILCGEQ LGMLGSACVV CEDCKKNVCT KCGVETSNNR PHPVWLCKIC LEQREVWKRS GAWFFKGFPK QVLPQPMPIK KTKPQQPAGE PATQEQPTPE SRHPARAPAR GDMEDRRPPG QKPGPDLTSA PGRGSHGPPT RRASEARMST AARDSEGWDH AHGGGTGDTS RSPAGLRRAN SVQAARPAPA PVPSPAPPQP VQPGPPGGSR ATPGPGRFPE QSTEAPPSDP GYPGAVAPAR EERTGPAGGF QAAPHTAAPY SQAAPARQPP PAEEEEEEAN SYDSDEATTL GALEFSLLYD QDNSNLQCTI IRAKGLKPMD SNGLADPYVK LHLLPGASKS NKLRTKTLRN TRNPVWNETL QYHGITEEDM QRKTLRISVC DEDKFGHNEF IGETRFSLKK LKANQRKNFN ICLERVIPMK RAGTTGSARG MALYEEEQVE RIGDIEERGK ILVSLMYSTQ QGGLIVGIIR CVHLAAMDAN GYSDPFVKLW LKPDMGKKAK HKTQIKKKTL NPEFNEEFFY DIKHSDLAKK SLDISVWDYD IGKSNDYIGG CQLGISAKGE RLKHWYECLK NKDKKIERWH QLQNENHVSS D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an essential role in docking and fusion steps of regulated exocytosis. At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release. In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability. Plays also a role in the exocytosis of arginine vasopressin hormone.
  • 基因功能参考文献：
    1. In mice, phosphorylation-regulated neutrophil polarization mechanism is driven by PKN1-mediated phosphorylation of a newly identified RAB21 effector RPH3A. PMID: 28636945
    2. Rph3A interacts with GluN2A and PSD-95 forming a complex that regulates NMDARs stabilization at postsynaptic membranes. PMID: 26679993
    3. Our results indicate that a decrease in mRNA levels underlie the depletion of protein levels of rabphilin 3A, and we suggest that this reduction may be involved in causing impaired synaptic transmission in R6/1 mice. PMID: 15967669
    4. rabphilin, by interacting with alpha-actinin, organizes the cell cytoskeleton to facilitate granule localization within F-actin-rich regions PMID: 16043482
    5. Binding of rabphilin to SNAP-25 regulates exocytosis of synaptic vesicles after the readily releasable pool has either been physiologically exhausted by use-dependent depression, or has been artificially depleted by deletion of synaptobrevin. PMID: 16763567
    6. Our results suggest that rabphilin, a protein present in presynaptic terminals, could play a role in the late phase of L-LTP. PMID: 17223267

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  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell projection, dendritic spine. Cell junction, synapse, postsynaptic cell membrane. Membrane; Peripheral membrane protein.
  • 组织特异性：
    Specifically expressed in brain.
  • 数据库链接：

    KEGG: mmu:19894

    STRING: 10090.ENSMUSP00000078198

    UniGene: Mm.181166