Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4,mitochondrial (Pdk4), partial

Recombinant Mouse [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4,mitochondrial (Pdk4), partial

In Stock
  • 中文名称：
    小鼠Pdk4重组蛋白
  • 货号：
    CSB-EP017730MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Pdk4
  • Uniprot No.：
  • 别名：
    Pdk4; [Pyruvate dehydrogenase; acetyl-transferring)] kinase isozyme 4; mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    30.0 kDa
  • 表达区域：
    138-368aa
  • 氨基酸序列
    ILEYKDTCTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSKTGNPSHIGSIDPNCDVVAVVQDAFECAKMLCDQYYLTSPELNLTQVNGKFPGQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENRPSLTPVEATVVLGKEDLTIKISDRGGGVPLRITDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSMSGYGTDAIIYLKALS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
  • 基因功能参考文献：
    1. In conclusion, our data indicated that PDK4 potentially contributes to the hepatic steatosis in NASH via regulating several signaling pathway and PDK4 may be a new therapeutic strategy against NAFLD. PMID: 29128353
    2. our results suggest that PDK2/4 can be a potential target for the development of pharmacotherapy for the treatment of acute inflammatory pain PMID: 26931482
    3. Taken together these data support a model where PDHK4 regulates KRAS signalling and its tumorigenic properties and suggest that inhibition of PDHK4 could represent a novel therapeutic strategy to target KRAS mutant colorectal and lung cancers PMID: 28692044
    4. PDK4 mediates cisplatin-induced acute kidney injury. PMID: 28040265
    5. Pyruvate dehydrogenase kinase 4 is a direct transcriptional target of FoxO1 in the heart. FoxO1 directly regulates Pdk4 transcription in the heart, thereby controlling PDH activity and subsequent glucose oxidation rates. PMID: 28687587
    6. inhibition of PDK4 activity in Hepatocellular carcinoma cells increased cyclin E1, cyclin A2, and E2F1 proteins. PMID: 28003426
    7. FXR may promote the proliferation of tumor cells and the hepatocytes in the process of liver regeneration by activating the PDK4-mediated metabolic reprogramming to generate glycolytic intermediates essential for rapid biomass generation, establishing a mechanistic link between cell proliferation and metabolic switch. PMID: 26728993
    8. Taken together, our results suggest that LPS induces PDK4 expression and alters glucose metabolism via the JNK pathway. PMID: 26740179
    9. upregulation of PDK4 promotes vascular calcification by increasing osteogenic markers with no adverse effect on bone formation, demonstrating that PDK4 is a therapeutic target for vascular calcification. PMID: 26560812
    10. PDK2/4 induction and the subsequent lactate surge induce the metabolic shift in the diabetic dorsal root ganglion thereby contributing to the pathogenesis of painful diabetic neuropathy. PMID: 26769971
    11. These findings reveal that there are divergent roles of PDKs during oocyte maturation and indicate a new mechanism controlling meiotic structure. PMID: 25991547
    12. Double-knockout mice with global deletion of PDK2 and PDK4, which results in constitutively activated pyruvate dehydrogenase, preferentially oxidize glucose in muscle. PMID: 25368185
    13. PDK4 promotes tumorigenesis through activation of the CREB-RHEB-mTORC1 signaling cascade. PMID: 25164809
    14. Diminished insulin signaling evident at 1 wk on the high fat diet did not occur in pyruvate dehydrogenase kinase 4 knockout mice. PMID: 24116221
    15. The Pdk4 gene knockdown led to better glucose tolerance than the Pdk2 gene knockdown. Hepatic Pdk4 may be critically involved in the pathogenesis of diabetes. PMID: 23940800
    16. These results suggest a role for PDK4 in regulating the PDH complex in muscle and promoting gluconeogenic precursor recirculation during recovery from exhaustive exercise. PMID: 24305065
    17. loss of both Pdk2 and Pdk4 attenuated HSC quiescence, glycolysis, and transplantation capacity. PMID: 23290136
    18. Deletion of PDK4 prevented Angiotensin II-induced diastolic dysfunction and normalized glucose oxidation to basal levels. PMID: 23396452
    19. the role of IL-6 signalling in adipose tissue during exercise PMID: 22844518
    20. Data suggest that up-regulation of cardiac PDK4 by high-fat diet (HFD) occurs in conjunction with time-dependent activation of E2F1 transcription factor (E2F1); HFD may initiate early cardiac metabolic alterations through cyclin D1/E2F1/PDK4 axis. PMID: 22569253
    21. PDK4 plays a role in reducing pyruvate dehydrogenase complex activation during low to moderate-intensity muscle stimulation, and that absence of PDK4 and the subsequent changes in carbohydrate utilization may alter force production. PMID: 22196220
    22. These findings indicate that Pdk4 plays an important role in bone loss at unloading by promoting osteoclastogenesis. PMID: 21803180
    23. PDK4 is a novel pivotal factor in adaptation to chemical stress. PMID: 21182459
    24. upregulation of PDK4 in skeletal muscle PMID: 12099888
    25. activation of FXR may suppress glycolysis and enhance oxidation of fatty acids via inactivation of the pyruvate dehydrogenase complex by increasing PDK4 expression PMID: 15721319
    26. PGC-1alpha expression in skeletal muscle coordinately upregulate the expression of pyruvate dehydrogenase kinase 4 (PDK4), a negative regulator of glucose oxidation. PMID: 16314495
    27. Pyruvate dehydrogenase kinase 4-deficient mice have lower fasting blood glucose levels, slightly improved glucose tolerance, and slightly greater insulin sensitivity compared with wild-type mice. PMID: 18430968
    28. PDK4 upregulation in adipocytes participates in the hypolipidemic effect of thiazolidinediones through modulation of glyceroneogenesis PMID: 18519799
    29. Regulation of the PDK4 isozyme by the Rb-E2F1 complex PMID: 18667418
    30. PDHK4 deficiency creates conditions that alter upstream signalling components involved in the regulation of lipid metabolism. PMID: 19627255
    31. The activation of pyruvate dehydrogenase kinase 4 during starvation is mediated by peroxisome proliferator-activated receptor alpha PMID: 11554740

    显示更多

    收起更多

  • 亚细胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    PDK/BCKDK protein kinase family
  • 数据库链接：

    KEGG: mmu:27273

    STRING: 10090.ENSMUSP00000019721

    UniGene: Mm.235547