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  4. Recombinant Mouse Protein lin-28 homolog B (Lin28b)

Recombinant Mouse Protein lin-28 homolog B (Lin28b)

  • 中文名称：
    小鼠Lin28b重组蛋白
  • 货号：
    CSB-YP673823MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Lin28b重组蛋白
  • 货号：
    CSB-EP673823MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Lin28b重组蛋白
  • 货号：
    CSB-EP673823MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Lin28b重组蛋白
  • 货号：
    CSB-BP673823MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Lin28b重组蛋白
  • 货号：
    CSB-MP673823MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Lin28b
  • Uniprot No.：
  • 别名：
    Lin28b; Protein lin-28 homolog B; Lin-28B
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-247
  • 氨基酸序列
    MAEGGASKGE EPEKLPGLAE DEPQVLHGTG HCKWFNVRMG FGFISMISRE GNPLDIPVDV FVHQSKLFME GFRSLKEGEP VEFTFKKSPK GLESIRVTGP GGSPCLGSER RPKGKTLQKR KPKGDRCYNC GGLDHHAKEC SLPPQPKKCH YCQSIMHMVA NCPHKLAAQL PASSQGRQEA ESQPCSSAAP REVGGGHGCT VLFPQEVKSE MAEHSDRSPQ EVSSTKAFAA IGEQNKKGPL IQKRKKT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression. When overexpressed, may stimulate growth of carcinoma cell lines.
  • 基因功能参考文献：
    1. Regulating the IL-1beta/miR-i101/Lin28B signaling pathway. PMID: 27562754
    2. The study deciphered the essential role of Lin28B in the pathogenesis of epithelial-mesenchymal transition in idiopathic pulmonary fibrosis, and unraveled a novel mechanism that miR-26a is a modulator of let-7d. PMID: 26787543
    3. Study generated transgenic mice that either overexpressed Lin28b in Math1-positive cerebellar granule neuron precursors or in a broad range of Nestin-positive neural precursors; while overexpression of LIN28B in Nestin-positive cells does not lead to tumor formation, it results in a protracted development of granule cells and inhibitory interneurons and leads to a hypersublobulation of the cerebellar vermis. PMID: 27039094
    4. this study shows that ectopic Lin28b expression reinitiates fetal regenerative potential in a subset of adult hematopoietic stem cells PMID: 27533015
    5. expression of the heterochronic factor Lin28b decreases in common myeloid progenitors during hematopoietic maturation to adulthood in mice. PMID: 27401346
    6. loss of Lin28b expression in fetal T cells leads to increased mature let-7, which causes decreased expression of TGF-betaRI, TGF-betaRIII, and SMAD2 proteins. A reduction in TGF-beta signaling leads to reduced Treg numbers. PMID: 27793996
    7. Lin28b positively regulates the expression of Lin28b itself and cell cycle-related proteins in hepatoblasts by suppressing the maturation of target microRNAs, lethal-7b and miR-125a/b, enabling maintenance of the stem cell properties of hepatoblasts. PMID: 26990797
    8. The identification of the let-7/Lin28 axis as an important regulator of HSC activation as well as its upstream modulators and down-stream targets will provide insights into the involvement of altered microRNA expression in contributing to the pathogenesis of alcoholic liver fibrosis PMID: 28536261
    9. Lin28B/let-7 axis acts as a critical driver of peripheral nervous system myelination PMID: 26466203
    10. These results implicate a key role for the LIN28B/let-7 axis in regulating postnatal supporting cell plasticity. PMID: 26139524
    11. Upregulation of LIN28B is associated with systemic mastocytosis. PMID: 25655194
    12. VH repertoire of Lin28b-induced BM B1a B cells differs from that of normal B1a, suggesting persisting differences from fetal progenitors. PMID: 25753579
    13. MYCN/LIN28B/Let-7/HMGA2 pathway implicated by meta-analysis of GWAS in suppression of post-natal proliferation thereby potentially contributing to aging PMID: 23639551
    14. intestine targeted expression of LIN28B causes intestinal hypertrophy, crypt expansion, and Paneth cell loss PMID: 24142874
    15. Rpl22 inactivation promotes neoplastic transformation by inducing expression of Lin28B PMID: 22976955
    16. Enforced expression of Lin28b leads to impaired T-cell development, release of inflammatory cytokines, and peripheral T-cell lymphoma. PMID: 22723554
    17. study reports Lin28b is expressed in fetal liver and thymus, but not adult bone marrow or thymus; ectopic expression of Lin28 reprograms hematopoietic progenitor cells from adult bone marrow, endowing them with ability to mediate fetal-like lymphopoiesis PMID: 22345399

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  • 亚细胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    Lin-28 family
  • 数据库链接：

    KEGG: mmu:380669

    UniGene: Mm.440328