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  4. Recombinant Mouse Protein disulfide-isomerase A3 (Pdia3)

Recombinant Mouse Protein disulfide-isomerase A3 (Pdia3)

  • 中文名称：
    小鼠Pdia3重组蛋白
  • 货号：
    CSB-YP017720MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pdia3重组蛋白
  • 货号：
    CSB-EP017720MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pdia3重组蛋白
  • 货号：
    CSB-EP017720MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pdia3重组蛋白
  • 货号：
    CSB-BP017720MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pdia3重组蛋白
  • 货号：
    CSB-MP017720MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pdia3; Erp; Erp60; Grp58; Protein disulfide-isomerase A3; EC 5.3.4.1; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-505
  • 氨基酸序列
    SDVLEL TDENFESRVS DTGSAGLMLV EFFAPWCGHC KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY PTLKIFRDGE EAGAYDGPRT ADGIVSHLKK QAGPASVPLR TEEEFKKFIS DKDASVVGFF RDLFSDGHSE FLKAASNLRD NYRFAHTNIE SLVKEYDDNG EGITIFRPLH LANKFEDKTV AYTEKKMTSG KIKKFIQDSI FGLCPHMTED NKDLIQGKDL LTAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA VASRKTFSHE LSDFGLESTT GEVPVVAIRT AKGEKFVMQE EFSRDGKALE QFLQEYFDGN LKRYLKSEPI PESNEGPVKV VVAENFDDIV NEEDKDVLIE FYAPWCGHCK NLEPKYKELG EKLSKDPNIV IAKMDATAND VPSPYEVKGF PTIYFSPANK KLTPKKYEGG RELNDFISYL QREATNPPII QEEKPKKKKK AQEDL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 基因功能参考文献：
    1. Role of the ERp57 protein (1,25D3-MARRS receptor) in murine mammary gland growth and development PMID: 29477346
    2. PDI knockout inhibits the inflammatory function of macrophages by decreasing ROS production and inactivating NF-kappaB pathway. PMID: 29294242
    3. Thiol isomerase ERp57 is mobilized to the surface of activated platelets via a process that requires actin polymerization. PMID: 27079884
    4. These data indicate a synergistic crosstalk between 1alpha,25(OH)2D3 and BMP2 toward osteogenesis and mineral deposition, involving both VDR and Pdia3. PMID: 23784946
    5. ERp57 deficiency causes neuromuscular defects PMID: 26869642
    6. ERp57 levels are increased in the airway epithelium of asthmatic patients and in mice with allergic airways disease. PMID: 26435004
    7. Data suggest that regulation of Pdia3 (protein disulfide isomerase associated 3 protein) expression by microRNA-330-5p is important in maintaining hair cycle through regulation of proliferation and migration capability of keratinocytes. PMID: 26402295
    8. All the growth plate and bone abnormalities, however, become attenuated after the pubertal growth spurt, when protein synthesis is decelerated and, hence, ERp57 function is less essential. PMID: 25704664
    9. ERp57 regulates thrombosis via multiple targets. PMID: 25156521
    10. These data suggest the 25D3 -MARRS receptor/PDIA3/ERp57 may contribute to the length of lifespan in mammals. PMID: 25283641
    11. Pdia3+/- mice exhibit a mild skeletal phenotype characterized by enlarged growth plates, reduced tether and trabecular bone formation, and impaired osteogenic potential of calvarial osteoblasts in vitro. PMID: 25405762
    12. Maternal PDIA3 plays a crucial role in sperm decondensation by reducing protamine disulfide bonds in porcine oocytes, supporting its utility as a potential tool for oocyte selection in assisted reproduction techniques. PMID: 25030891
    13. Cell cycle analyses demonstrated that the suppressed expression of ERp57 increased the sub-G1 population. These data reveal that increased expression of ERp57 may contribute to the protection from beta cell death caused by autophagic failure. PMID: 25241190
    14. The current study implicates PDIA3 to be an important cellular neuroprotective mechanism against a toxic drug PMID: 22715419
    15. Pdia3 co-localizes with vitamin D receptor and caveolin-1 on the surface of MC3T3-E1 osteoblasts. In response to 1alpha,25(OH)2D3, receptors translocate and change interactions. PMID: 23896121
    16. These data indicate that both the chaperone functional domains and the subcellular location of Pdia3 control rapid membrane responses to 1alpha,25-dihydroxyvitamin d3. PMID: 23660595
    17. Platelets lacking ERp57 have defective activation of the alphaIIbbeta3 integrin and platelet aggregation. The defect in aggregation was corrected by the addition of exogenous ERp57, implicating surface ERp57 in platelet aggregation. PMID: 24030382
    18. Knockout of the Pdia3 protein in mice eliminates the rapid response to calcitriol in intestinal epithial cells. PMID: 22546984
    19. Phospholipase A2 activating protein is required for 1alpha,25-dihydroxyvitamin D3 dependent rapid activation of protein kinase C via Pdia3 PMID: 22484374
    20. ERp57 has roles in multiple components of platelet function and arterial thrombus formation. PMID: 22168334
    21. ERp57 is required for platelet aggregation, hemostasis, and thrombosis. PMID: 22207737
    22. ERp57, by ER luminal interaction with STIM1, has a modulatory role in capacitative Ca(2+) entry. PMID: 21941299
    23. ERp57 participates in signal transduction from the cell surface, in regulatory processes taking place in the nucleus, and in multimeric protein complexes involved in DNA repair. (Review) PMID: 21837552
    24. 1,25D(3)-MARRS receptor is of central importance to steroid hormone-stimulated calcium uptake in mammalian intestinal cells. PMID: 20682787
    25. Pdia3 plays a crucial role in 1alpha,25(OH)2D3-regulated bone formation and the Pdia3-PKC signalling pathway might be involved in this process PMID: 20576531
    26. ERp57 effects on STAT3 signaling are enhanced by ER luminal complex formation between ERp57 and calreticulin PMID: 20022947
    27. The thiol oxidoreductase endoplasmic reticulum (ER)p57 interacts with newly synthesized glycoproteins through ternary complexes with the chaperones/lectins calnexin or calreticulin. PMID: 14978212
    28. ERp57 plays an important role during the post-translational phases of glycoprotein folding, when the primary requirement for promotion of glycoprotein maturation is an isomerase/reductase activity PMID: 16407314
    29. ERp57 is involved in disulfide formation in vivo but not in peptide loading of class I molecules PMID: 16567808
    30. Overall, these results suggest that protein folding and other activities mediated through PDI and BiP are diminished during aging. PMID: 17996725
    31. the function of ERp57 in peptide loading is likely caused by other ERp57 functional domains or a combinatorial feature of the tapasin-ERp57 conjugate PMID: 18650385
    32. ERP57 is a key protein that controls immunogenicity by controlling calreticulin exposure and serves as a molecular marker of immunogenicity. PMID: 18684944
    33. ERp57 must be physically associated with the calnexin cycle to catalyze isomerization reactions with most of its substrates PMID: 19054761

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  • 亚细胞定位：
    Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome.
  • 蛋白家族：
    Protein disulfide isomerase family
  • 组织特异性：
    In caput and cauda epididymal spermatozoa, detected in the acrosome and principal piece (at protein level).
  • 数据库链接：

    KEGG: mmu:14827

    STRING: 10090.ENSMUSP00000028683

    UniGene: Mm.263177