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  4. Recombinant Mouse Protein Wnt-7a (Wnt7a)

Recombinant Mouse Protein Wnt-7a (Wnt7a)

  • 货号：
    CSB-YP026141MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026141MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026141MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026141MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Wnt7a; Wnt-7a; Protein Wnt-7a
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    32-349
  • 氨基酸序列
    LGASIICNK IPGLAPRQRA ICQSRPDAII VIGEGSQMGL DECQFQFRNG RWNCSALGER TVFGKELKVG SREAAFTYAI IAAGVAHAIT AACTQGNLSD CGCDKEKQGQ YHRDEGWKWG GCSADIRYGI GFAKVFVDAR EIKQNARTLM NLHNNEAGRK ILEENMKLEC KCHGVSGSCT TKTCWTTLPQ FRELGYVLKD KYNEAVHVEP VRASRNKRPT FLKIKKPLSY RKPMDTDLVY IEKSPNYCEE DPVTGSVGTQ GRACNKTAPQ ASGCDLMCCG RGYNTHQYAR VWQCNCKFHW CCYVKCNTCS ERTEMYTCK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation. Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes. Required for normal neural stem cell proliferation in the hippocampus dentate gyrus. Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation. Promotes formation of synapses via its interaction with FZD5.
  • 基因功能参考文献：
    1. Wnt7a induces a unique phenotype of monocyte-derived macrophages with lower phagocytic capacity and differential expression of pro- and anti-inflammatory cytokines PMID: 28872671
    2. experiments indicate that Reck and Gpr124 are part of the cell surface protein complex that transduces Wnt7a- and Wnt7b-specific signals in mammalian CNS epithelial cells to promote angiogenesis and regulate the BBB PMID: 28803732
    3. WNT7a is sufficient to restore post-pubertal endometrial gland formation. PMID: 27965056
    4. we found that post-ejaculated semen from fertile wild-type males was solidified and the sperm were entrapped in Wnt7aCre/+;Esr1f/f uteri, compared to the watery semen (liquefied) found in Esr1f/f controls.Kallikrein-related peptidases 3 (KLK3) and other kallikrein-related peptidases from male prostate glands are responsible for semen liquefaction by cleaving gel-forming proteins PMID: 28414719
    5. Data show that the WIF domain of Wnt Inhibitory Factor 1 (WIF1) is bound by C-terminal domains of Wnt proteins Wnt5a and Wnt7a at two sites. PMID: 26342861
    6. Wnt7a is a novel regulator of ventral midbrain neurogenesis and dopaminergic neuron axon growth and guidance. PMID: 24803261
    7. Truncated Wnt7a lacking the conserved palmitoylation sites retains full biological activity in skeletal muscle. PMID: 24287629
    8. epithelial Wnt7a and Wnt7b are possible ligands of Fzd-mediated beta-catenin (Ctnnb1)-dependent (canonical) Wnt signaling in the adjacent undifferentiated periotic mesenchyme of fibrocytes in the murine inner ear PMID: 24727668
    9. Binding of Wnt7a to Fzd7 leads to an activation of noncanonical Wnt signaling, resulting in directed myogenic stem cell migration and enhanced engraftment. PMID: 24711502
    10. Wnt7a triggers melanocyte stem cell differentiation through beta-catenin activation, and Kitl might induce following migration of melanoblasts to epidermis PMID: 23702581
    11. Wnt7a exercised critical control over multiple steps of neurogenesis by regulating genes involved in both cell cycle control and neuronal differentiation. PMID: 23629626
    12. FN and Wnt7a together regulate the homeostatic levels of satellite stem cells and satellite myogenic cells during regenerative myogenesis. PMID: 23290138
    13. Hyperactivated c-Met led to increased NF-kappaB signaling, which in turn, drove de novo expression of Wnt7a and overexpression of Wnt7b in Pkd1(-/-) mouse kidneys. PMID: 22677559
    14. Our data demonstrate that Wnt7a is the earliest known gamma-motor neuron marker PMID: 22723712
    15. These results indicate that WNT7A plays a critical role in postnatal uterine gland morphogenesis and function, which are important for blastocyst implantation and fertility in the adult uterus. PMID: 21508348
    16. Data show that female Wnt7a-Cre(+)PR(f/-) mice are infertile due to defects in embryo attachment, stromal cell decidualization, and the inability to cease estrogen-induced epithelial cell proliferation. PMID: 22155565
    17. Data show that the Fzd7 receptor complex was associated with Galpha(s) and PI(3)K and these components were required for Wnt7a to activate the Akt/mTOR growth pathway in myotubes. PMID: 22179044
    18. Wnt-7a and sonic hedgehog (Shh) interfere differently with the process of neuronal differentiation by promoting distinct stages of neuronal differentiation in neonatal neural stem cells. PMID: 20868729
    19. Data show that removal of canonical Wnt inhibitor DKK1 from DKK1-involuted mice results in full recovery, suggesting that canonical Wnt signaling is required for the differentiation or proliferation of TEC populations. PMID: 20161711
    20. Wnt7a coordinates a variety of cell and developmental pathways that guide postnatal uterine growth and hormonal responses and that disruption of these pathways leads to aberrant cell death. PMID: 15070830
    21. stage-specific physiological roles of Wnt signaling during neural development. PMID: 15142975
    22. Wnt7a acts through low density lipoprotein receptor-related protein 6 and the canonical Wnt signaling pathway during dorsal and posterior limb development in the mouse PMID: 15880584
    23. Thus, our findings suggest that Wnt7a overexpression disrupts normal Wnt signaling in the neural tube, resulting in defective adherens junctions and neurulation. PMID: 16154760
    24. We show that loss of function of Dishevelled-1 (Dvl1) mimics and enhances the Wnt7a phenotype in the cerebellum.Wnt7a/Dvl1 double mutant mice reveal a defect in neurotransmitter release at mossy fiber-granule cell synapses PMID: 16818724
    25. Results suggest a critical role for Wnt7a in control of neuronal progenitor maturation. PMID: 17174942
    26. Wnt7a induces dissociation of the adenomatous polyposis coli protein from the beta-catenin cytoplasmic complex and the interaction of APC with alpha 7-nicotinic acetylcholine receptors in hippocampal neurons. PMID: 17507554
    27. CNS uses the canonical Wnt signaling pathway to promote formation and CNS-specific differentiation of the organ's vasculature PMID: 19023080
    28. Heterozygous and homozygous Vangl2(Lp) mutant postnatal tissues exhibit similar phenotypes and polarity defects and display a 50% reduction in Wnt7a levels PMID: 19363157
    29. Wnt7a signaling through the planar cell polarity pathway controls the homeostatic level of satellite stem cells and hence regulates the regenerative potential of muscle PMID: 19497282

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  • 相关疾病：
    Defects in Wnt7a cause the postaxial hemimelia (px) phenotype that is characterized by limb patterning defects accompanied by Mullerian duct-associated sterility in both sexes.
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 数据库链接：

    KEGG: mmu:22421

    STRING: 10090.ENSMUSP00000032180

    UniGene: Mm.56964