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  4. Recombinant Mouse Protein THEMIS (Themis)

Recombinant Mouse Protein THEMIS (Themis)

  • 货号：
    CSB-YP804787MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP804787MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP804787MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP804787MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP804787MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Themis
  • Uniprot No.：
  • 别名：
    Themis; Protein THEMIS; Grb2-associating protein; Gasp; Protein thylex; Thymocyte-expressed molecule involved in selection
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-636
  • 氨基酸序列
    MALSLEEFVY SLDLRTLPRV LEIQSGIYFE GSVYEMFGNE CCLSTGEVIK ITGLKIKKMM AEICEGAIGG CESQKPFELP MNFPGLFKVM ADKTPYLSIE EITRTVNIGP SRLGHPCFYH LKDIKLENLI IKQGEPIRFN SVEEINGETL VNCGVVRNHQ SHSFTLPLSQ EGEFYECEDE HIYTLKEIVE WKIPKNRTRT VKLTDFSNKW DSTNPFPEDF YGTLILKPVY EIQGVLKFQK DIVRILPSLD VEVKDITDSY DANWFLQLLS TDDLFEMTSK EFPVVAEVVE ISQGNHLPQS ILQREKTIVI HKKYQASRIL ASEIRSNFPK RHFLIPISYK GKFKRRPREF PTAYDLQIAK SRKETLHVVA TKAFHTLHKE LSPVSVGDQF LVHHSETTEV VFEGTRKVNV LTCEKVLNKT REDAQLPLYM EGGFVEVIHD KKQYQISELC TQFCWPFNVK VAVRDLSIKD DILAATPGLQ LEEDITDSYL LISDFANPEE CWEIPMSRLN MTVRLVNGSS LPADAGLLQV RSFVEEITEE QYYMMRRYES SLSHPPPRPP KHPSAEEMKL TLLSLAEERT INLPKSLKSH HVDRPKKLPS DESGQDSRAP VGFQNDVADV ERQKSKHGPL QPQAPL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
  • 基因功能参考文献：
    1. this study demonstrates the potential role of Themis1 in the suppressive functions of regulatory T cells. PMID: 28373295
    2. Themis1 acts as a positive regulator of TCR signaling during thymocyte development by promoting Vav1 activity and Grb2 stability PMID: 27188442
    3. the positive selection of immature CD4+CD8+ double-positive thymocytes and their commitment to the CD4+CD8- single-positive stage are impaired in Themis-/- mice PMID: 28877990
    4. we found that THEMIS directly regulated the catalytic activity of the tyrosine phosphatase SHP-1. PMID: 28250424
    5. The results demonstrate an essential requirement for the PRR and NLS motifs but not the conserved CABIT cysteines for Themis1 function. PMID: 24935457
    6. THEMIS is required for the development and ultimately the function of proinflammatory T cells. PMID: 25452553
    7. the THEMIS:SHP complex dampens early TCR signalling by a previously unknown molecular mechanism that favours T-cell survival. PMID: 25535246
    8. by dampening mild T-cell receptor signals Themis increases the affinity threshold for activation, enabling positive selection of T cells with a naive phenotype in response to low-affinity self-antigens PMID: 24226767
    9. specific role for THEMIS in early TCR signalosome function PMID: 21189249
    10. Themis plays a unique role in sustaining positive selection PMID: 19597497
    11. Themis functions to sustain and/or integrate signals required for proper lineage commitment and maturation. PMID: 19597498
    12. Themis had high expression in thymocytes between the pre-T cell antigen receptor and positive-selection checkpoints and low expression in mature T cells. Themis-deficient T-cells showed defective positive selection, resulting in fewer mature thymocytes. PMID: 19597499
    13. Study described a gene called Gasp that is critical for positive selection. PMID: 19805304
    14. Gasp associates with Grb2 and is required for positive selection of thymocytes PMID: 19805304
    15. Themis is a member of a new metazoan gene family and is required for the completion of thymocyte positive selection PMID: 19597497

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Themis family
  • 组织特异性：
    Expressed in the thymus and to a lesser extent in the spleen but not detectable in non-lymphoid tissues. Highly expressed in thymocytes between the pre-T-cell antigen receptor (pre-TCR) and positive-selection checkpoints and expressed at low level in matu
  • 数据库链接：

    KEGG: mmu:210757

    STRING: 10090.ENSMUSP00000060129

    UniGene: Mm.123021