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  4. Recombinant Mouse Protein N-terminal glutamine amidohydrolase (Wdyhv1)

Recombinant Mouse Protein N-terminal glutamine amidohydrolase (Wdyhv1)

  • 中文名称：
    小鼠Wdyhv1重组蛋白
  • 货号：
    CSB-YP773920MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Wdyhv1重组蛋白
  • 货号：
    CSB-EP773920MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Wdyhv1重组蛋白
  • 货号：
    CSB-EP773920MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Wdyhv1重组蛋白
  • 货号：
    CSB-BP773920MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Wdyhv1重组蛋白
  • 货号：
    CSB-MP773920MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Wdyhv1
  • Uniprot No.：
  • 别名：
    Wdyhv1; Ntaq1; Protein N-terminal glutamine amidohydrolase; EC 3.5.1.122; Protein NH2-terminal glutamine deamidase; N-terminal Gln amidase; Nt(Q)-amidase; WDYHV motif-containing protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-209
  • 氨基酸序列
    MEGDGPAATA PQYQPVCPTR DACVYNSCYC EENIWKLCEY IKTHNQYLLE ECYAVFISNE KKMVPIWKQQ ARPENGPVIW DYHVVLLHVS REGQSFIYDL DTILPFPCPF DIYIEDALKS DDDIHLQFRR KFRVVRADSY LKHFASDRSH MKDSSGNWRE PPPEYPCIET GDSKMNLNDF ISMDPAVGWG AVYTLPEFVH RFSSKTYQA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
  • 基因功能参考文献：
    1. Site-directed mutagenesis of Ntaq1 and its crystal structure indicate that the active site and catalytic mechanism of Nt(Q)-amidase are similar to those of transglutaminases. PMID: 19560421
  • 亚细胞定位：
    Cytoplasm, cytosol. Nucleus.
  • 蛋白家族：
    NTAQ1 family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    KEGG: mmu:76773

    STRING: 10090.ENSMUSP00000064622

    UniGene: Mm.26771