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  4. Recombinant Mouse Protein AF-9 (Mllt3)

Recombinant Mouse Protein AF-9 (Mllt3)

  • 中文名称：
    小鼠Mllt3重组蛋白
  • 货号：
    CSB-YP014635MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Mllt3重组蛋白
  • 货号：
    CSB-EP014635MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Mllt3重组蛋白
  • 货号：
    CSB-EP014635MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Mllt3重组蛋白
  • 货号：
    CSB-BP014635MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Mllt3重组蛋白
  • 货号：
    CSB-MP014635MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Mllt3
  • Uniprot No.：
  • 别名：
    Mllt3; Af9Protein AF-9; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-569
  • 氨基酸序列
    MASSCAVQVK LELGHRAQVR KKPTVEGFTH DWMVFVRGPE HSNIQHFVEK VVFHLHESFP RPKRVCKDPP YKVEESGYAG FILPIEVYFK NKEEPKKVRF DYDLFLHLEG HPPVNHLRCE KLTFNNPTED FRRKLLKAGG DPNRSIHTSS SSSSSSSSSS SSSSSSSSSS SSSSSSSSSS SSSSSSSSSS TSFSKPHKLM KEHKEKPSKD SREHKSAFKE PSRDHNKSSK DSSKKPKENK PLKEEKIVPK MAFKEPKPMS KEPKADSNLL TVTSGQQDKK APSKRPPASD SEELSAKKRK KSSSEALFKS FSSAPPLILT CSADKKQIKD KSHVKMGKVK IESETSEKKK SMLPPFDDIV DPNDSDVEEN MSSKSDSEQP SPASSSSSSS SSFTPSQTRQ QGPLRSIMKD LHSDDNEEES DEAEDNDNDS EMERPVNRGG SRSRRVSLSD GSDSESSSAS SPLHHEPPPP LLKTNNNQIL EVKSPIKQSK SDKQIKNGEC DKAYLDELVE LHRRLMTLRE RHILQQIVNL IEETGHFHIT NTTFDFDLCS LDKTTVRKLQ SYLETSGTS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones. Recruitment of the SEC complex to crotonylated histones promotes recruitment of DOT1L on active chromatin to deposit histone H3 'Lys-79' methylation (H3K79me). Plays a key role in hematopoietic stem cell (HSC) maintenance by preserving, rather than confering, HSC stemness. Acts by binding to the transcription start site of active genes in HSCs and sustaining level of H3K79me2, probably by recruiting DOT1L.
  • 基因功能参考文献：
    1. Atg5-dependent autophagy contributes to the development of acute myeloid leukemia in an MLL-AF9-driven mouse model. PMID: 27607576
    2. YEATS domain of AF9 directly links histone crotonylation to active transcription. PMID: 27105114
    3. Data suggest that RAS-homolog enriched in brain protein (Rheb1) promotes MLL-AF9 fusion protein initiated acute myeloid leukemia (AML) progression through target of rapamycin complex 1 (mTORC1) signaling pathway. PMID: 27071307
    4. Using a PDK1 conditional deletion MLL-AF9 murine AML model, we revealed that the deletion of PDK1 prolonged the survival of AML mice by inducing LSC apoptosis PMID: 25769952
    5. define a specific pairing of two amino acids that creates a salt bridge between MLLT1/3 and AFF proteins that is critically important for MLL-mediated transformation of HPCs PMID: 25282333
    6. Af9 mediates site-selective physical and functional recruitment of Rnf2 to the alpha-ENaC promoter to constrain basal alpha-ENaC transcription in collecting duct cells. PMID: 24070375
    7. Impaired alphaENaC expression due to failure to inhibit Dot1a-Af9 may play an important role in the early stages of pseudohypoaldosteronism type 1 in MR(-/-) mice. PMID: 24026182
    8. We demonstrate that leukemogenic activity of MLL-AF9 requires RUVBL2 (RuvB-like 2), an AAA+ ATPase family member that functions in a wide range of cellular processes, including chromatin remodeling and transcriptional regulation. PMID: 23403462
    9. Therefore, Dot1a and Af9 as aldosterone-downregulated targets are negative regulators of endothelin-1 transcription in vitro and in vivo, and may be considered as new potential therapeutic targets of kidney injury in diabetes. PMID: 23077601
    10. It was concluded that +78/+92 of the epithelial Na+ channel alpha-subunit represents the primary Af9 binding site involved in recruiting Dot1a to repress basal and aldosterone-sensitive Na+ channel alpha-subunit transcription. PMID: 23152297
    11. PRC2 supports aberrant self-renewal in a mouse model of MLL-AF9;Nras(G12D) acute myeloid leukemia. PMID: 22469984
    12. The role of polycomb repressive complex 2 (PRC2) function in leukemia development and progression, is reported. PMID: 22396593
    13. Data show that the minimal 90-amino acid AF9 fragment in MLL-AF9 retains an ability to form higher order complexes with AF4*P-TEFb and with DOT1. PMID: 21896721
    14. Dot1l, an H3K79 methyltransferase, is required for both initiation and maintenance of MLL-Af9-induced leukemogenesis in vitro and in vivo. PMID: 21521783
    15. Af9/Mllt3 interferes with Tbr1 expression through epigenetic modification of histone H3K79 during development of the cerebral cortex PMID: 20348416
    16. A shift in AF9 from the nucleus to the cytoplasm in response to Hsp90 interference leads to increased ENaCalpha expression. This is accompanied by a decrease in AF9 occupancy at the ENaCalpha promoter. PMID: 20159978
    17. Af9 gene is required for normal embryogenesis in mice by controlling pattern formation, apparently via control of Hox gene regulation PMID: 12242306
    18. Dot1a-AF9 modulates histone H3 Lys-79 methylation at the ENaCalpha promoter and represses ENaCalpha transcription in an aldosterone-sensitive manner PMID: 16636056
    19. Af9 is a physiologic target of Sgk1, and Sgk1 negatively regulates the Dot1a-Af9 repressor complex that controls transcription of ENaCalpha and likely other aldosterone-induced genes. PMID: 17332896
    20. Overlapping expression of Af9 with Af4/Aff1 and Fmr2/Aff2, two genes that are also related to neurodevelopmental diseases, as well as with the highly homologous Enl. PMID: 19000783
    21. AF17 competes with AF9 to bind Dot1a, decreases Dot1a nuclear expression by possibly facilitating its nuclear export, and relieves Dot1a.AF9-mediated repression of alpha-ENaC and other target genes. PMID: 19864429

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 组织特异性：
    Ubiquitously expressed. Strong expression in the spleen.
  • 数据库链接：

    KEGG: mmu:70122

    STRING: 10090.ENSMUSP00000077232

    UniGene: Mm.288898