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  4. Recombinant Mouse Prostaglandin-H2 D-isomerase (Ptgds)

Recombinant Mouse Prostaglandin-H2 D-isomerase (Ptgds)

  • 中文名称：
    小鼠Ptgds重组蛋白
  • 货号：
    CSB-YP018969MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ptgds重组蛋白
  • 货号：
    CSB-EP018969MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ptgds重组蛋白
  • 货号：
    CSB-EP018969MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ptgds重组蛋白
  • 货号：
    CSB-BP018969MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ptgds重组蛋白
  • 货号：
    CSB-MP018969MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ptgds
  • Uniprot No.：
  • 别名：
    Ptgds; Prostaglandin-H2 D-isomerase; EC 5.3.99.2; Glutathione-independent PGD synthase; Lipocalin-type prostaglandin-D synthase; Prostaglandin-D2 synthase; L-PGDS; PGD2 synthase; PGDS2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-189
  • 氨基酸序列
    QGHDTV QPNFQQDKFL GRWYSAGLAS NSSWFREKKA VLYMCKTVVA PSTEGGLNLT STFLRKNQCE TKIMVLQPAG APGHYTYSSP HSGSIHSVSV VEANYDEYAL LFSRGTKGPG QDFRMATLYS RTQTLKDELK EKFTTFSKAQ GLTEEDIVFL PQPDKCIQE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophobic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. Involved in PLA2G3-dependent maturation of mast cells. PLA2G3 is secreted by immature mast cells and acts on nearby fibroblasts upstream to PTDGS to synthesize PGD2, which in turn promotes mast cell maturation and degranulation via PTGDR.
  • 基因功能参考文献：
    1. These observations suggest that tumor cell-derived inflammatory cytokines increase L-PGDS expression and subsequent PGD2 production in the tumor endothelial cells (ECs). This PGD2 acts as a negative regulator of the tumorigenic changes in tumor ECs. PMID: 29124765
    2. Exposure of macrophages to cyclooxygenase (COX-2) inhibitors RhoA and LA-4 cells to antagonists of prostaglandin E2 (PGE2) receptor , PGD2 receptors or the hepatocyte growth factor (HGF) receptor c-Met (PHA-665752), reversed EMT inhibition by the conditioned medium PMID: 26875548
    3. Ptgds is targeted and repressed by the CUL4B/PRC2 complex. PMID: 26025376
    4. Thermodynamic and nuclear magnetic resonance analyses demonstrated that L-PGDS binds to nicotinamide coenzymes, including NADPH, NADP(+), and NADH. PMID: 26518650
    5. Prostaglandin D2 synthase is a novel MR target gene in adipocytes. PMID: 25966493
    6. When prostaglandin D2 synthase is upregulated it participates in the regulation of peripheral nervous system myelination. PMID: 25362470
    7. expression of L-PGDS in mouse lungs decreased neutrophilic infiltration, ameliorating lung inflammation in mice. PMID: 24029383
    8. Silencing of PTGDS also alleviated H-rev107-mediated suppression of cell migration and invasion PMID: 23687991
    9. In this report, we show for the first time that alteration of the PTGDS-governed PGD2 pathway disturbs testicular migration in mice. PMID: 23076868
    10. The levels of lipocalin-type prostaglandin D2 synthase, and its product PGD2 each varied significantly among background strains of mice after wounding, and the highest Ptgds and PGD2 levels were associated with the lowest amount of regeneration. PMID: 23190891
    11. The lipocalin-type prostaglandin D2 KO mice show early elevations in HPA activity with no age-related changes relative to the C57BL/6 mice. PMID: 23151358
    12. Results suggest that L-PGDS plays an important role in the regulation of glucose utilization in vivo. PMID: 22923471
    13. Findings demonstrate that the backbone conformation of the bottom region of mouse lipocalin-type prostaglandin D synthase is still maintained in the intermediate state. PMID: 22210903
    14. Negative regulation of the adipogenesis program by the enhanced expression of L-PGDS through a cellular mechanism involving the interference of the PPARgamma signaling pathway without the contribution of endogenous pro-adipogenic prostanoids. PMID: 22100987
    15. PGD2 is produced in articular tissue during the development of collagen-induced arthritis and plays an antiinflammatory role, acting through the DP1 receptor. PMID: 21898357
    16. results suggest the importance of selenium in the shunting of AA metabolism toward the production of PGD(2) metabolites, which may have clinical implications PMID: 21669866
    17. These results suggest that the stably reduced expression levels of L-PGDS regulates positively adipogenesis program in a cellular mechanism independent of pro-adipogenic action of PGJ(2) series. PMID: 21726533
    18. Study suggests that PGD(2) derived from L-PGDS-expressing cells plays proinflammatory roles in colitis. PMID: 21163901
    19. L-PGDS may fine-tune the retinoic acid signalling in melanocytes. PMID: 20403807
    20. PGD synthase is elevated in the urine and kidneys of mice during anti-glomerular basement membrane disease, as well as in mice with spontaneously arising lupus nephritis. PMID: 20065116
    21. The expression of H-PGDS in human dendritic cells (DCs) and the regulatory mechanisms by which DCs produce prostaglandin D2, is demonstrated. PMID: 20008150
    22. Expression of prostaglandin D synthase in ovarian cancer. PMID: 11798075
    23. The protein is present throughout the epididymis, except in the initial segment; a major protein in caudal fluid;an N-glycosylated, highly polymorphic protein; and conserved during evolution. PMID: 11804971
    24. enzyme is characterized, folded and unfolded. PMID: 12441340
    25. Estradiol differentially regulates its transcript levels in the rodent brain: evidence from high-density oligonucleotide arrays and in situ hybridization PMID: 12518068
    26. PGD synthase in brain microglia peaked at postnatal day (PND) 10, decreased gradually thereafter, and reached a plateau at PND 20. Target molecules of PGD2, i.e., DP receptor and CRTH2 receptor, showed developmental profiles similar to PGD synthase. PMID: 12673832
    27. Crystallization and preliminary X-ray analysis of lipocalin-type prostaglandin D synthase. PMID: 12761195
    28. prostaglandin D synthase in the preoptic area of female mice has a role in arousal and sex behavior PMID: 14638944
    29. Hematopoietic prostaglandin D synthase is produced by microglia until 72 h after reperfusion, but at 7 days after reperfusion, it is also produced by migrating bone marrow/blood-derived macrophages in the ischemic brain tissue. PMID: 17241746
    30. Liver X receptor-mediates up-regulation of L-PGDS through activation of SREBP-1c is a novel pathway to enhance adipocyte differentiation PMID: 17439953
    31. L-PGDS induction was regulated positively by AP-1, but negatively by p53. PMID: 17675519
    32. NMR solution structure of L-PGDS: evidence for partial overlapping of catalytic pocket and retinoic acid-binding pocket within the central cavity. PMID: 17715133
    33. the thermal unfolding monitored by CD spectroscopy at 200, 235 and 290 nm revealed that L-PGDS unfolded through the intermediate state, where its main chain retained the characteristic beta-sheet structure without side-chain interactions PMID: 18076651
    34. 15-PGJ(2) is synthesized during mammalian inflammatory responses PMID: 18077391
    35. L-PGDS is an important mediator of muscle and adipose glucose transport which is modulated by glycemic conditions and plays a significant role in the glucose intolerance associated with type 2 diabetes. PMID: 18619553
    36. l-pgds deficiency induces obesity and facilitates atherosclerosis, probably through the regulation of inflammatory responses. PMID: 19070593
    37. Biochemical, functional, and pharmacological characterization of AT-56, an orally active and selective inhibitor of lipocalin-type prostaglandin D synthase. PMID: 19131342
    38. Our findings suggest that L-PGDS is beneficial for protecting the brain against transient and permanent cerebral ischemia. PMID: 19254753
    39. Overexpression of prostaglandin D synthase augments inflammation followed by muscle injury in muscular dystrophy. PMID: 19359520
    40. This cardioprotective effect of dexamethasone was markedly reduced in L-PGDS-deficient mice PMID: 19451694
    41. L-PGDS expression is induced in the myocardium under hypoxemia, which may reflect the adaptation to the hemodynamic stress. PMID: 19470375
    42. analysis of the structural basis of the catalytic mechanism operating in open-closed conformers of lipocalin type prostaglandin D synthase PMID: 19546224
    43. First report of insulin resistance, glucose intolerance, and early atherosclerosis associated with L-PGDS knockout. PMID: 15970590

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  • 亚细胞定位：
    Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm, perinuclear region. Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. In the male reproductive system, it is expressed in the testis, efferent ducts and epididymis, and is secreted into the
  • 数据库链接：

    KEGG: mmu:19215

    STRING: 10090.ENSMUSP00000015234

    UniGene: Mm.1008