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  4. Recombinant Mouse Probable threonine protease PRSS50 (Prss50), partial

Recombinant Mouse Probable threonine protease PRSS50 (Prss50), partial

  • 货号：
    CSB-YP806400MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP806400MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806400MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806400MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806400MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Prss50
  • Uniprot No.：
  • 别名：
    Prss50; Tsp50Probable threonine protease PRSS50; EC 3.4.25.-; Serine protease 50; Testis-specific protease-like protein 50
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your protein CSB-EP806400MO, could you please advise on the lead time, the sequencing, the pricing, and the expected tag information?
Also, have any customers reported this protein to have any activity?

A:
Thanks for your inquiry.
Expression Region: 48-415aa; Partial, provide the complete extracellular domain.
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

ERIRPRRPPRHAHPRPPLTRWRPSTGYLAAGASPGTLSTTVPTGPGVSCGSRGICPSGRLRLPRQAQTNQTTTAPPNSQTMAPLKTVGTLGMMDTTGSVLKTVHSSNLPFCGSSHEPDPTLRDPEAMTRRWPWMVSVQANGSHICAGILIASQWVLTVAHCLSQNHVNYIVRAGSPWINQTAGTSSDVPVHRVIINHGYQPRRYWSWVGRAHDIGLLKLKWGLKYSKYVWPICLPGLDYVVEDSSLCTVTGWGYPRANGIWPQFQSLQEKEVSILNSKKCDHFYHKFSRISSLVRIINPQMICASDNNREEFCYEITGEPLVCSSDGTWYLVGMMSWGPGCKKSEAPPIFLQVSYYRPWIWDRLSGEP


Regarding the activity, sorry, we haven't received the feedback from other customers, and we also haven't detected the activity before, it's not 100% guaranteed.
However, as we use the affinity chromatography method to purify in a mild mild condition, theoretically, it should be active. We recommend you to purchase a small size to have a try first.

靶点详情

  • 功能：
    May be involved in proteolysis through its threonine endopeptidase activity.
  • 基因功能参考文献：
    1. TSP50 expression at embryonic day 6.5 (E6.5), day 8.5 (E8.5) and day 10.5 (E10.5) was increasingly enhanced, However, the expression of TSP50 decreased gradually in the development and differentiation of cardiac myocyte from E12.5 to postnatal (P0). PMID: 29842883
    2. findings indicate that BORIS-induced expression of TSP50 is governed by accessibility and binding of BORIS to the promoter PMID: 21659515
    3. TSP50 promotes cell proliferation, at least partially, through activation of the NF-kappaB signalling pathway. PMID: 21385156
    4. results showed that downregulation of TSP50 expression in P19 embryonal carcinoma stem cells not only reduced cell proliferation, colony formation, and migration but also induced cell apoptosis PMID: 21086474
    5. TSP50 may perform a critical biologic function in mammalian spermatogenesis. It may be a new type of threonine protease whose linear catalytic structure is similar to many serine proteases, except for a Thr-for-Ser residual catalytic site substitution. PMID: 15491742
    6. These findings suggested that DNA methylation might regulate the TSP50 and mTSP50 gene expressions in different types of tissues and spermatic cells. PMID: 18662669

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：

    KEGG: mmu:235631

    STRING: 10090.ENSMUSP00000059668

    UniGene: Mm.102265