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  4. Recombinant Mouse Probable folate receptor delta (Folr4)

Recombinant Mouse Probable folate receptor delta (Folr4)

  • 中文名称：
    小鼠Izumo1r重组蛋白
  • 货号：
    CSB-YP875340MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Izumo1r重组蛋白
  • 货号：
    CSB-EP875340MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Izumo1r重组蛋白
  • 货号：
    CSB-EP875340MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Izumo1r重组蛋白
  • 货号：
    CSB-BP875340MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Izumo1r重组蛋白
  • 货号：
    CSB-MP875340MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Izumo1r; Folbp3; Folr4; Juno; Sperm-egg fusion protein Juno; Folate receptor 4; Folate receptor delta; FR-delta; Folate-binding protein 3; IZUMO1 receptor protein JUNO
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-244
  • 氨基酸序列
    G DKLLSVCMNS KRHKQEPGPE DELYQECRPW EDNACCTRST SWEAHLEEPL LFNFSMMHCG LLTPACRKHF IQAICFHECS PNLGPWIQPV VPNGQEEQRV WGVPLCQEDC EDWWRACHSS LTCKSNWLHG WDWSEEKKHC PAHEPCLPFS YHFPTPDDLC EKIWNNTFKA SPERRNSGRC LQKWFEPTLS NPNVEVALHF AGSALAPQLS YTLPAFSLCL LFHP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for species-specific gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate.
  • 基因功能参考文献：
    1. JUNO interacts with IZUMO1 during sperm binding.Trp62 of JUNO interacts with hydrophobic residues on the alpha-helical core region of IZUMO1.Surface-exposed tryptophan residue of JUNO is required for sperm binding and fertilization. PMID: 27416963
    2. Juno was expressed in oocytes at the germinal vesicle stage and it continues to be expressed at similar levels in later stages of oocytes maturation. PMID: 29124919
    3. JUNO associates with monomeric IZUMO1, which is then quickly removed as tight adhesion of the two cells is subsequently established. PMID: 26568141
    4. Binding of sperm protein Izumo1 and its egg receptor Juno drives Cd9 accumulation in the intercellular contact area prior to fusion during mammalian fertilization. PMID: 25209248
    5. Of Juno, neuropeptide release, and light-activated chloride channels. [review] PMID: 24863928
    6. identification of folate receptor 4 (Folr4) as the receptor for Izumo1 on the mouse egg, and propose to rename it Juno; female mice lacking Juno are infertile and Juno-deficient eggs do not fuse with normal sperm PMID: 24739963
    7. Distinct from the expression profile of conventional TFH markers, FR4 was highly expressed by naive CD4(+) T cells, was downregulated after activation and subsequently re-expressed on TFH cells. PMID: 24030473
    8. performed gene expression profiling experiments on uterus tissue of pregnant mice with either wildtype alleles or targeted disruption at the folate receptor 4 locus PMID: 23651732
    9. In this report, a Treg-depleting anti-FR4 antibody is combined with a GM-CSF-secreting tumor cell immunotherapy for treatment of melanoma-bearing animals. PMID: 23811319
    10. This study describes a novel FR4 transcript variant, FR4D3, in which exon 3 is deleted. The mRNA of FR4D3 encodes a FR4 variant truncated by 189 bp. FR4D3 was found to be predominantly expressed in CD4(+)CD25(+) Treg cells. PMID: 22694797
    11. novel splice variant of FR4 predominantly expressed in CD4+CD25+ regulatory T cells PMID: 19860584
    12. Regulatory T cells (Treg) express high amounts of folate receptor 4, which distinguishes Treg from other naive or activated T cells. PMID: 17613255

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Folate receptor family
  • 组织特异性：
    Widely expressed with higher expression in thymus, spleen and lung. Present at the cell surface of unfertilized oocytes, while it is barely detectable 30 to 40 minutes after fertilization (at protein level).
  • 数据库链接：

    KEGG: mmu:64931

    STRING: 10090.ENSMUSP00000034409

    UniGene: Mm.86738