Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Probable carboxypeptidase PM20D1 (Pm20d1)

Recombinant Mouse Probable carboxypeptidase PM20D1 (Pm20d1)

  • 货号：
    CSB-YP806954MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP806954MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806954MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806954MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806954MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pm20d1
  • Uniprot No.：
  • 别名：
    Pm20d1; N-fatty-acyl-amino acid synthase/hydrolase PM20D1; EC 3.5.1.-; EC 4.3.-.-; Peptidase M20 domain-containing protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-503
  • 氨基酸序列
    STGPRS RENRGASRIP SQFSEEERVA IKEALKGAIQ IPTVSFSHEE SNTTALAEFG EYIRKAFPTV FHSSLVQHEV VAKYSHLFTI QGSDPSLQPY MLMAHIDVVP APEEGWEVPP FSGLERNGFI YGRGALDNKN SVMAILHALE LLLIRNYSPK RSFFIALGHD EEVSGEKGAQ KISALLQARG VQLAFLVDEG SFILEGFIPN LEKPVAMISV TEKGALDLML QVNMTPGHSS APPKETSIGI LSAAVSRLEQ TPMPNMFGGG PLKKTMKLLA NEFSFPINIV LRNLWLFHPI VSRIMERNPI TNALVRTTTA LTMFNAGIKV NVIPPLAQAT INCRIHPSQT VHEVLELVKN TVADDRVQLH VLRSFEPLPI SPSDDQAMGY QLLQETIRSV FPEVDIVVPG ICIANTDTRH YANITNGMYR FNPLPLNPQD FSGVHGINEK VSVQNYQNQV KFIFEFIQNA DTYKEPVPHL HEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction (Probable). Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles.
  • 基因功能参考文献：
    1. These data demonstrate that PM20D1 is a dominant enzymatic regulator of NAA levels in vivo and elucidate physiologic functions for NAA signaling in metabolism and nociception. PMID: 29967167
    2. Study demonstrates that PM20D1 is a bidirectional enzyme in vitro, catalyzing both the condensation of fatty acids and amino acids to generate N-acyl amino acids and also the reverse hydrolytic reaction. N-acyl amino acids directly bind mitochondria and function as endogenous uncouplers of UCP1-independent respiration. PMID: 27374330
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase M20A family
  • 组织特异性：
    In addition to being detected in blood (at protein level), PM20D1 is also highly expressed in other tissues including brown adipocytes, liver and kidney. It is also expressed in small intestine, large intestine, heart and pancreas.
  • 数据库链接：

    KEGG: mmu:212933

    STRING: 10090.ENSMUSP00000046079

    UniGene: Mm.23844