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  4. Recombinant Mouse Pro-opiomelanocortin (Pomc)

Recombinant Mouse Pro-opiomelanocortin (Pomc)

  • 中文名称：
    小鼠Pomc重组蛋白
  • 货号：
    CSB-YP365527MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pomc重组蛋白
  • 货号：
    CSB-EP365527MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pomc重组蛋白
  • 货号：
    CSB-EP365527MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pomc重组蛋白
  • 货号：
    CSB-BP365527MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pomc重组蛋白
  • 货号：
    CSB-MP365527MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pomc; Pomc1Pro-opiomelanocortin; POMC; Corticotropin-lipotropin) [Cleaved into: NPP; Melanotropin gamma; Gamma-MSH); Corticotropin; Adrenocorticotropic hormone; ACTH); Melanocyte-stimulating hormone alpha; Alpha-MSH; Melanotropin alpha); Corticotropin-like intermediary peptide; CLIP); Lipotropin beta; Beta-LPH); Lipotropin gamma; Gamma-LPH); Melanocyte-stimulating hormone beta; Beta-MSH; Melanotropin beta); Beta-endorphin; Met-enkephalin]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    27-100
  • 氨基酸序列
    WCLE SSQCQDLTTE SNLLACIRAC KLDLSLETPV FPGNGDEQPL TENPRKYVMG HFRWDRFGPR NSSSAGSAAQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Stimulates the adrenal glands to release cortisol.; Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes.; Increases the pigmentation of skin by increasing melanin production in melanocytes.; Endogenous orexigenic opiate.; Endogenous opiate.
  • 基因功能参考文献：
    1. s show that renal-denervated WT and diabetic mice recapitulate the phenotype of improved glucose tolerance and elevated glycosuria associated with reduced renal GLUT2 levels observed in obese ArcPomc(-/-) mice. PMID: 29031726
    2. Alpha - MSH enhanced leptin sensitivity and preadipocyte proliferation, meanwhile inhibited endoplasmic reticulum stress of preadipocytes by activating Notch1 signal. PMID: 27717825
    3. Glutamate transporter-associated protein 3-18 (GTRAP3-18), an anchor protein that retains interacting proteins in the endoplasmic reticulum, is a critical regulator of food intake and body weight by interacting with POMC. GTRAP3-18-deficient mice showed hypophagia, lean bodies, and lower blood glucose, insulin, and leptin levels with increased serum and brain alpha-MSH levels, leading to AMPK inhibition. PMID: 28904020
    4. Immunostaining of serial sections of the hypothalamus revealed that POMC-expressing neurons were smaller in WNK1 TG mice than in WT mice. PMID: 29392534
    5. Selenoprotein T is a novel oligosaccharyltransferase subunit that regulates unfolded protein response signaling and ACTH secretion. PMID: 28928140
    6. Study shows that alpha melanocyte stimulating hormone and forkhead box C2 protein promote fatty acid oxidation through C/EBPbeta negative transcription in mice adipose tissue. PMID: 27819350
    7. Our findings demonstrate alphaMSH plays a key role in the prevention of adipose inflammation and inflammatory diseases by down-regulating Akt/JNK signal pathway and negatively interacting with FoxOs, which brings up alphaMSH as a novel candidate factor in the adipose anti-inflammation process in obesity. PMID: 28514752
    8. Data indicate the regulation of energy balance in BMPR1A-mediated appetite regulation in POMC neurons. PMID: 29040444
    9. POMC and AgRP neurons receive direct steroid- and frequency-dependent glutamatergic synaptic input from Kiss1(ARC) neurons in male mice PMID: 27093227
    10. Localization of the dopamine receptors of types 1 and 2 on the bodies of POMC-expressing neurons of the arcuate nucleus of the hypothalamus in mice and rats.( PMID: 28429261
    11. These findings indicate that the relevance of AgRP to POMC neuron GABA connectivity depends on the state of AgRP neuron activity and suggest that different types of transmitter release should be considered when circuit mapping. PMID: 28667175
    12. To clarify the functional roles of the AKT-mTOR pathway in the hippocampal neurons, we generated conditional knockout mice harboring the deletion of Pten (Pten-cKO) in Proopiomelanocortin-expressing neurons. PMID: 26961412
    13. Epo regulates hypothalamus POMC expression via STAT3 activation. PMID: 26563310
    14. Data suggest that the solitary tract pro-opiomelanocortin (NTSPOMC) neurons were involved in cardiorespiratory control and endogenous analgesia. PMID: 27077912
    15. hypothalamic arcuate nucleus (Arc)POMC-deficient mice, which develop severe obesity and insulin resistance, unexpectedly exhibit improved glucose tolerance and remain protected from hyperglycemia via decreased renal GLUT2 expression and glycosuria. PMID: 26467632
    16. Hypomethylation of POMC promoter occurred in the hypothalamus of the high sucrose diet offspring compared with controls. PMID: 25936720
    17. Alpha-MSH may play an important role in the itching associated with pigmented cutaneous lesions and that the histamine released from keratinocytes is involved in this alpha-MSH-induced itching. PMID: 26358220
    18. These data indicate that glucose induces distinct excitatory synaptic plasticity in different subpopulations of POMC neurons. PMID: 25127258
    19. Although genetic deletion of either Atg12 or Atg5 renders POMC neurons autophagy-deficient, mice lacking Atg5 in POMC neurons do not exhibit these phenotypes. PMID: 25585051
    20. these results reveal that cooperative interactions between the enhancers confer robustness of Pomc expression against gene regulatory disturbances and preclude deleterious metabolic phenotypes caused by Pomc deficiency in adulthood PMID: 25671638
    21. In contrast to mice with total hypothalamic Pomc deficiency, which are severely obese, mice with Lepr-restricted Pomc expression displayed fully normal body weight, food consumption, glucose homeostasis, and locomotor activity. PMID: 25594696
    22. Data suggest FoxO1 (forkhead box O1) region Gly140-Leu160 (Gln145, Arg147, Lys148, Arg153, Arg154) is critical for FoxO1 interaction with Stat3 (signal transducer/activator of transcription 3) in down-regulation of leptin-induced Pomc transcription. PMID: 25510553
    23. increased plasma ACTH levels in chronic psychosocially stressed male mice PMID: 24376791
    24. Data (including data from knockout mice) suggest that mobilization of neutrophils during stress requires expression of annexin A1 and likely involves ACTH-mediated activation/up-regulation of various receptors on surface membrane of neutrophils. PMID: 25184992
    25. ACTH activates Brown adipose tissue and browning of white adipose tissue while corticosterone counteracts this PMID: 25085924
    26. we demonstrate that POMC gene delivery suppressed melanoma growth by inducing apoptosis, which was at least partly dependent on Nox4 upregulation. PMID: 24412703
    27. alpha-MSH overexpression in the nucleus tractus solitarius decreases fat mass and elevates heart rate. PMID: 24829220
    28. Data indicate that early-life exposure to ethanol disrupts circadian oscillations of gene expression of proopiomelanocortin (POMC) and metabolic genes in POMC neurons. PMID: 24797626
    29. Rab3a deficiency promotes constitutive secretion and underlies selective impairment of Ca(2)-dependent release of alpha-MSH PMID: 24205339
    30. Data suggest changes in DNA methylation regulate expression of pituitary Pomc; CpG site-specific methylation of Pomc promoter represses mRNA transcription; reduction in DNA methylation occurs with delay after early-life stress and persists 1 year. PMID: 24506071
    31. postnatal ablation of POMC neurons leads to enhanced anxiety and the development of obesity despite decreased food intake and glucocorticoid deficiency. PMID: 23676213
    32. Gamma-2MSH directly regulates both ENaCalpha expression and cellular localization in the inner medulla to exert its natriuretic effect. PMID: 23871693
    33. This study demonstrated that both glutamatergic and GABAergic cells comprise significant, distinct subpopulations of hypothalamic POMC neurons. PMID: 23640796
    34. Data indicate the cellular and molecular mechanisms that underlie proopiomelanocortin (POMC) neuronal function under normal and perturbed physiological conditions in immortalized neuronal cell lines. PMID: 24134870
    35. NF-kappaB suppresses food intake and energy expenditure in mice by directly activating the Pomc promoter PMID: 23370526
    36. alpha-MSH and NPY modulate phagocytic activity in macrophages. PMID: 23689030
    37. Our results show dynamic changes in POMC in hypothalamic and extra-hypothalamic regions that may contribute to the negative affective/emotional state of heroin withdrawal PMID: 23337531
    38. The s show a negative correlation between miR-375 and Pomc expression and suggest that miR-375 is a novel negative regulator of POMC expression and related hormone secretion. PMID: 23430746
    39. Additional studies demonstrate that arcuate nucleus pro-opiomelanocortin (POMC) expressing neurons are unresponsive to leptin PMID: 23341784
    40. In females, adult exposure to the high fat diet increased POMC expression in the arcuate nucleus. There was no change for males. There were no effects of BPA or DES exposure. PMID: 23493373
    41. Cordycepin decreased the phosphorylation of CREB induced by alpha-MSH and IBMX in B16F10 melanoma cells. PMID: 21972008
    42. Pomc expression has an effect on diverse immature neuronal populations PMID: 22166984
    43. symptoms of atopic dermatitis such as scratching can be exacerbated by alpha-MSH, which is induced by iNOS-derived NO PMID: 21895774
    44. The coordinate expression of Etv1 with POMC cell differentiation and its interaction with the highly cell-restricted Tpit factor indicate that Etv1 participates in a combinatorial code for pituitary cell-specific gene expression. PMID: 21622576
    45. Apelin and the proopiomelanocortin system: a new regulatory pathway of hypothalamic alpha-MSH release. PMID: 21846903
    46. SGK1 is strongly stimulated by glucocorticoids in pituitary corticotrophs; however, its effects on POMC transcription are antagonistic to the classical inhibitory glucocorticoid action PMID: 21586695
    47. Pituitary expression of POMC gene relies on a upstream enhancer that complements the activity of the proximal promoter with Tpit as the major regulator of both regulatory regions. The enhancer is conserved among species but not in hormone responsiveness. PMID: 21193556
    48. These data show that PTP1B in proopiomelanocortin neurons plays a role in short-term cold-induced reduction of spontaneous physical activity PMID: 21406615
    49. Data show that the phenotype of a mouse lacking both POMC and AgRP (DKO) is indistinguishable from that of mice lacking Pomc alone. PMID: 21363936
    50. The results shed light on the sex dependent differences in brain functioning and the role of Sry in the beta endorphin system related to the higher frequency of autistic disorder in males. PMID: 21408198

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    POMC family
  • 组织特异性：
    ACTH and MSH are produced by the pituitary gland.
  • 数据库链接：

    KEGG: mmu:18976

    STRING: 10090.ENSMUSP00000020990

    UniGene: Mm.277996