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  4. Recombinant Mouse Prion-like protein doppel (Prnd)

Recombinant Mouse Prion-like protein doppel (Prnd)

  • 中文名称：
    小鼠Prnd重组蛋白
  • 货号：
    CSB-BP886153MO
  • 规格：
    ￥2112
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Prnd
  • Uniprot No.：
  • 别名：
    Prnd; Prion-like protein doppel; Doppelganger; Dpl; PrPLP
  • 种属：
    Mus musculus(Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Baculovirus
  • 分子量：
    18.9 kDa
  • 表达区域：
    27-155aa
  • 氨基酸序列
    RGIKHRFKWNRKVLPSSGGQITEARVAENRPGAFIKQGRKLDIDFGAEGNRYYAANYWQFPDGIYYEGCSEANVTKEMLVTSCVNATQAANQAEFSREKQDSKLHQRVLWRLIKEICSAKHCDFWLERG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for normal acrosome reaction and for normal male fertility. Can bind Cu(2+).
  • 基因功能参考文献：
    1. in GC-1 spg cells, phosphorylation of p21 and N-terminal truncated PrP may play roles in the control of Dpl-induced apoptosis, which may benefit the physiological function of Dpl in the male reproduction system. PMID: 24339999
    2. Data suggest that glycosylation status of the prion protein and yet-to-be-identified proteases modulate internal C1 and C2 endoproteolysis of Doppel (doppelganger prion) and Shadoo (shadow of prion protein) in mouse neurons. PMID: 24286250
    3. Data on residue secondary structure propensities suggest that novel beta-sheets of doppel protein (Prnd) and prion protein (Prnp) are formed by amino acids belonging to helices that are the least stable in the respective native structures. PMID: 24143866
    4. This finding identifies a protein domain that plays a role in mediating Dpl-related toxicity. PMID: 23345215
    5. Findings raise the possibility that Bax and caspase-3 feature in Dpl-mediated apoptosis. PMID: 22561161
    6. These results indicated that Dpl was glycosylated in a cell type- and tissue-specific manner regardless of PrP(C), while PrP(C) endoproteolysis was modulated by Dpl expression. PMID: 21144827
    7. ectopic expression of PrP-like protein Doppel in central neurons induces significant Purkinje cell death resulting in late-onset ataxia PMID: 19055638
    8. We suggest a mechanism for Doppel-mediated Purkinje cell degeneration linked to reduced gene expression of proteins related to neuronal activity. PMID: 20219645
    9. interaction between Dpl and PrPC occurs in lipid rafts and is dependent on the integrity of these membrane microdomains PMID: 19888917
    10. Expression of doppel in the CNS of mice does not modulate transmissible spongiform encephalopathy disease PMID: 11842265
    11. Ectopic PrPLP/Dpl in the absence of PRNP is actively involved in the glial-cell activation in the brain. PMID: 11844868
    12. Differences between the prion protein and its homolog Doppel: a partially structured state with implications for scrapie formation PMID: 11866533
    13. absence causes male sterility PMID: 12110578
    14. tested whether Doppel and PrP(C) share the same cell location, thereby sharing the same neighboring cell components, probably required to share the same cell function PMID: 12387871
    15. Prion and doppel proteins bind to granule cells of the cerebellum, suggesting a scenario in which granule cells express a protein that mediates Dpl-induced neurodegeneration. PMID: 12446843
    16. Dpl protein binds copper, which may serve to modulate the activity, stability, or localization of the Dpl protein PMID: 12482851
    17. Doppel expression is not modified in scrapie-infected cells. PMID: 12586339
    18. findings provide evidence that the N-terminal residues 23-88 of prion protein containing the unique octapeptide-repeat region is crucial for preventing Purkinje cell death in PrP deficient mice expressing Doppel in the neuron PMID: 12759361
    19. The mechanism of toxicity involved stimulation of nitric oxide production via activation of the nitric oxide synthases, nNOS and iNOS. PMID: 12799144
    20. transgenic mice selectively expressing Dpl in Purkinje cells showed ataxia and Purkinje cell loss that depended on the level of Dpl expression PMID: 15007176
    21. Dpl compensates for the loss of Prion protein function in mutant mouse lines, but it does have an important anti-oxidant function necessary for sperm integrity and male fertility. PMID: 15161660
    22. Data show that Doppel protein expressed by Purkinje cells itself is toxic to the cells, and that the neurotoxicity is stoichiometrically antagonized by prion protein. PMID: 15194501
    23. Doppel interacts with the full-length laminin receptor precursor protein PMID: 15246873
    24. Cis-acting elements at the -182/-177 (E box) and -108/-104 (CCAAT box) positions were identified as the main activators of the Prnd expression in testis PMID: 15358521
    25. genetic studies show that N-terminal modules in PrPC offset pro-apoptotic activity of the Doppel helix B/B' region in mice PMID: 15459186
    26. Purkinje cell degeneration in protein-deficient mice is associated with cerebellar Prnd protein. PMID: 15862314
    27. These results indicate that Dpl elicited dose-dependently toxic effects on PrP-deficient cells without affecting on PrP-expressing cells, suggesting that the PrP-Dpl interaction can regulate cell death in a cell-autonomous manner. PMID: 15950943
    28. Loss of cellular prion protein renders hippocampal neurons susceptible to ischemic insult in male but not female mice. Ectopic expression of prion protein-like protein/Doppel aggravates ischemic neuronal death in female prion protein-null mice. PMID: 16198494
    29. Involvement of upstream stimulatory factor in regulation of the mouse Prnd gene coding for Doppel protein PMID: 16297464
    30. Doppel is expressed early during ontogenesis, and is found in both germ cells and Sertoli cells. Doppel may play a physiological role in acrosome biogenesis. PMID: 16421231
    31. Abnormal expression of PrPLP/doppel in PrP-knockout mice is caused by functional dissociation between the pre-mRNA machineries, in particular those of cleavage/polyadenylation and splicing. PMID: 17034959
    32. PrP(c) is involved with a response mediated by inflammation (paw edema) and by visceral conditioning stimuli. PMID: 17433806
    33. ectopic expression of doppel induces both BAX-dependent and BAX-independent pathways of cell death PMID: 17443816
    34. The finding that discrete lesions within the central nervous systems induced marked modulation of immune function probably indicates the existence of a delicately balanced neural-endocrine network by PrP(C) and PrPLP/Doppel. PMID: 17498663
    35. Results suggest that non-Bax-dependent pathways mediate the toxic effects of Dpl in Purkinje cells, highlighting a possible role for nonapoptotic mechanisms in the death of these neurons. PMID: 17569776
    36. Mitral cell loss and gliosis induced by ectopic Dpl expression were probably associated with the late-onset olfactory deficits in Rikn Prnp-/- mice. PMID: 17611634
    37. a possible model for the antagonistic interaction between PrP and Dpl PMID: 19158506
    38. Prion protein paralog doppel protein interacts with alpha-2-macroglobulin PMID: 19536284

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Prion family
  • 组织特异性：
    Detected in testis. Detected within seminiferous tubules, on round and elongated spermatids (at protein level). Not detected in brain (at protein level). Detected in testis, and at low levels in heart. Expression in brain is very low and barely detectable
  • 数据库链接：

    KEGG: mmu:26434

    STRING: 10090.ENSMUSP00000105798

    UniGene: Mm.180750