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  4. Recombinant Mouse Prestin (Slc26a5), partial

Recombinant Mouse Prestin (Slc26a5), partial

  • 中文名称：
    小鼠Slc26a5重组蛋白
  • 货号：
    CSB-YP859577MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Slc26a5重组蛋白
  • 货号：
    CSB-EP859577MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Slc26a5重组蛋白
  • 货号：
    CSB-EP859577MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Slc26a5重组蛋白
  • 货号：
    CSB-BP859577MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Slc26a5重组蛋白
  • 货号：
    CSB-MP859577MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Slc26a5
  • Uniprot No.：
  • 别名：
    Slc26a5; Pres; Prestin; Solute carrier family 26 member 5
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site.
  • 基因功能参考文献：
    1. response of endogenously expressed prestin in HEI-OC1 cells is different from the response of prestin expressed exogenously in non-auditory cells PMID: 26854618
    2. The IVS2-2A>G mutation in the Slc26a5 gene is insufficient to cause hearing loss in mice. PMID: 27232762
    3. Prestin expression imparts susceptibility to 2-hydroxypropyl-beta-cyclodextrin-induced hearing loss. PMID: 26903308
    4. Prestin missense mutations reduce outer hair cell survival in knockin mice. PMID: 26682723
    5. demonstrate that OHC lateral wall structure constrains the mobility of plasma membrane proteins and that the integrity of such membrane-associated structures are critical for Slc26a5's active and structural roles PMID: 26352669
    6. SPAG6 is indispensible for the stability of outer hair cells by maintaining the normal expression of prestin PMID: 25748314
    7. prestin is up-regulated by 32-58% in residual outer hair cell after noise exposure and that the prestin is functional. PMID: 24376553
    8. The findings suggest that CASK and the truncated prestin splice isoform contribute to confinement of prestin to the basolateral region of the plasma membrane. PMID: 23542924
    9. calmodulin-prestin interaction may be involved in the medial olivocochlear-mediated modulation of cochlear amplification PMID: 24453323
    10. prestin subunits are individually functional within a given multimer PMID: 23212912
    11. Prestin has a role in harnessing the basilar membrane as the source of cochlear frequency tuning. PMID: 21959151
    12. prestin knockout mice had increased auditory threshold but no changes in spine density or morphological characteristics on apical dendrites of auditory cortical layer 5 pyramidal neurons. PMID: 21773053
    13. A previously unexpected role of prestin, in mediating antibiotic-induced apoptosis, the effect of which is associated with its anion-transporting capacity. PMID: 21364896
    14. a molecular mechanism of prestin with at least two voltage-dependent conformational transition steps having distinct electromechanical coupling efficiencies. PMID: 21071769
    15. Data show that two prestin monomers are expressed on the cell surface, but only one is functional, that prestin dimerization is leads to increased voltage sensitivity, and that prestin maturation results in a depolarizing shift in voltage operating range. PMID: 20631244
    16. These data suggest that VAPA could be involved in prestin's transportation inside outer hair cells (OHCs) and may facilitate the targeting of this abundant OHC protein to the plasma membrane. PMID: 20359505
    17. Interaction between CFTR and prestin (SLC26A5). PMID: 20138822
    18. Results suggest that the majority of the intra-membrane protein particles are prestin and that electrically evoked length and stiffness changes are interrelated and dependent on both prestin and on the cortical actin cytoskeletal lattice. PMID: 20169529
    19. targeted deletion of prestin in mice results in loss of outer hair cell electromotility in vitro and a 40-60 dB loss of cochlear sensitivity in vivo, without disruption of mechano-electrical transduction in outer hair cells PMID: 12239568
    20. Genomic characterization and expression of mouse prestin PMID: 12584604
    21. Although prestin is expressed in vestibular hair cells, it is unlikely that it supports the form of somatic motility observed in outer hair cells. PMID: 14553901
    22. Results demonstrate that the absence of prestin, not hair-cell loss, is the primary cause of high-frequency hearing threshold elevation in prestin-/- and +/- mice. PMID: 15207913
    23. Compound action potentials in prestin knockout mice show a frequency-dependent, sloping loss between 35 and 60 decibels (dB). PMID: 15319415
    24. Results from F3-F5 generation mice suggest that one copy of the prestin gene is capable of compensating for the deleted copy and that heterozygous mice do not suffer peripheral hearing impairment. PMID: 16166160
    25. The dimer may act as the building block for producing the higher order oligomers that form the approximately 11-nM particles in the outer hair cell's basolateral membrane. PMID: 16682411
    26. Because mammals possess differentiated outer hair cells (OHC), they also benefit from a novel electromotile process, powered by the motor protein, prestin. PMID: 16873410
    27. Voltage-dependent, nonlinear capacitance, which represents the conformational fluctuations of the motor molecule prestin, progressively increases during development of the outer hair cells. PMID: 17415610
    28. The linkage shown between the loss of prestin protein and abnormal cochlear function validates the original knockout and attests to the importance of OHC motor function in the auditory periphery. PMID: 17664869
    29. Cholesterol levels modulate the distribution of prestin within plasma membrane microdomains and affect prestin self-association PMID: 17933870
    30. Prestin is crucial for defining sharp and sensitive cochlear frequency tuning by reducing the sensitivity of the low-frequency tail of the tuning curve. PMID: 18221877
    31. electrically evoked otoacoustic emissions are generated by prestin-based somatic motility PMID: 18234980
    32. needed for coupling between basilar membrane and inner hair cell PMID: 18397741
    33. prestin-based electromotility is required for cochlear amplification PMID: 18466744
    34. Prestin introduced into prestin-deficient outer hair cells segregates normally to the cell membrane and generates a normal nonlinear capacitance, indicative of normal prestin function. PMID: 18506528
    35. we describe a chimera analysis of prestin function and demonstrate a proportional change in cochlear physiology and sensitivity as the number of prestin-containing outer hair cells increases; i.e., genotype ratio and phenotype are closely related PMID: 19776286

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    SLC26A/SulP transporter (TC 2.A.53) family
  • 组织特异性：
    Expressed in the outer hair cells (OHC) of the organ of Corti of the inner ear. Also weak expression in brain and testis. Very weakly expressed in heart, spleen, muscle and lactating mammary glands.
  • 数据库链接：

    KEGG: mmu:80979

    STRING: 10090.ENSMUSP00000030878

    UniGene: Mm.160370