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  4. Recombinant Mouse Presenilin-1 (Psen1), partial

Recombinant Mouse Presenilin-1 (Psen1), partial

  • 中文名称：
    小鼠Psen1重组蛋白
  • 货号：
    CSB-YP018846MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Psen1重组蛋白
  • 货号：
    CSB-EP018846MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Psen1重组蛋白
  • 货号：
    CSB-EP018846MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Psen1重组蛋白
  • 货号：
    CSB-BP018846MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Psen1重组蛋白
  • 货号：
    CSB-MP018846MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Psen1; Ad3h; Psnl1; Presenilin-1; PS-1; EC 3.4.23.-; Protein S182) [Cleaved into: Presenilin-1 NTF subunit; Presenilin-1 CTF subunit; Presenilin-1 CTF12; PS1-CTF12)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the presence of the other members of the gamma-secretase complex for protease activity. Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels. Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin). Under conditions of apoptosis or calcium influx, cleaves CDH1. This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling. Required for normal embryonic brain and skeleton development, and for normal angiogenesis. Mediates the proteolytic cleavage of EphB2/CTF1 into EphB2/CTF2. The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is involved in calcium homeostasis. Involved in the regulation of neurite outgrowth. Is a regulator of presynaptic facilitation, spike transmission and synaptic vesicles replenishment in a process that depends on gamma-secretase activity. It acts through the control of SYT7 presynaptic expression.
  • 基因功能参考文献：
    1. found a stronger requirement for Presenilin during mouse lens development PMID: 30059908
    2. Data indicate a novel role for PS1 in regulating TREM2 intracellular trafficking and pathophysiological function. PMID: 29611543
    3. PS1 phosphorylated at Ser367 has an antiamyloidogenic function, promoting autophagosome-lysosome fusion and increasing betaCTF degradation PMID: 28533369
    4. Fibroblast growth factor (FGF1 and FGF2), but not vascular endothelial growth factor (VEGF) rescued Psen1-/- cells from serum starvation induced apoptosis.In the absence of serum, FGF2 immunoreactivity was distributed diffusely in cytoplasmic and nuclear vesicles of wt and Psen1-/- cells, as levels of FGF2 in nuclear and cytosolic fractions were not significantly different. PMID: 27443835
    5. Upregulated p53-p21 Signaling is linked with presenilin 1 mutants PMID: 27644130
    6. The cognitive function of APP/PS1 mice was impaired at 10months of age; moreover, the hypermetabolic state identified in various brain regions at 5months of age was also significantly decreased. PMID: 29107091
    7. Using knockout cell lines in combination with siRNA and immunoprecipitation approaches, our data clearly demonstrated that the Pen-2 and PS1 are sufficient to form a functionally active gamma-secretase that is capable of catalyzing the processing of Notch. This finding strongly suggests that Pen-2 is the most crucial component in gamma-secretase complex in addition to presenilin that functions as the catalytic subunit. PMID: 28234257
    8. that reduction in the synaptotagmin 1 level and presenilin 1-synaptotagmin 1 interactions in AD brain may present molecular underpinning of the pathogenic presenilin 1 conformation PMID: 28193235
    9. PS1 plays a crucial role in the cerebrovascular system and the vascular reactivity is decreased through altered Ca(2+) signals in PS1dE9 mutant mice. PMID: 28753475
    10. Loss of PS1 is associated with cognitive impairment. PMID: 27049828
    11. Cultured hippocampal neurons expressing mutant PS1 had attenuated CCE that was associated with destabilized dendritic spines, which were rescued by either gamma-secretase inhibition or overexpression of STIM1. PMID: 27601731
    12. Conditionally targeted deletion of PSEN1 leads to diastolic heart dysfunction and ultrastructural cardiomyocyte abnormalities. PMID: 28617969
    13. Tspan3 is a central endocytic membrane component regulating the expression of ADAM10, presenilin and the amyloid precursor protein. PMID: 27818272
    14. Study shows that the binding of HSF-1, Cdx1, Ets-1 and Sp1 to Presenilin 1 promoter and that of Nkx2.2, HFH-2, Cdx1 and NF-kappaB to Presenilin 2 promoter regulate their differential expression during brain development. PMID: 27177724
    15. conclusion that the PSEN1 mutations are extreme examples of the previously proposed ''dysfunction'' of gamma-secretase that characterizes familial Alzheimer's disease PMID: 27100199
    16. PS1 expression is triggered by glutamate through p38alpha, contributing to the excitotoxic stimulus of EW PMID: 27278235
    17. amino acid sensing of mechanistic target of rapamycin complex 1 (mTORC1) is dysregulated in cells deficient in presenilin. PMID: 26923592
    18. the G206D mutation reduced presenilin-1-presenilin enhancer 2 interaction, but did not abolish gamma-secretase formation and presenilin-1 endoproteolysis PMID: 25394380
    19. This study deals with the effect of attenuated endoproteolysis of PS1 on store-operated calcium (SOC) entry in neuronal cells and mouse fibroblasts with double knockouts of PS1 and PS2 PMID: 26678016
    20. Its mutation in methionine 146 impairs homeostatic synaptic plasticity. PMID: 26455952
    21. Results showed that epigenetic mechanisms play a pivotal role in transcriptional regulation of PS1 and PS2 during cerebral cortical development PMID: 25652771
    22. lactate levels remained elevated with age and increased aerobic glycolysis enzyme expression correlated with poorer memory performance in APP/PS1 mice PMID: 26865611
    23. young mouse WT splenocytes not only prevented AD, but also improved the spatial learning and memory of APPswe/PSENldE9 transgenic mice PMID: 26317549
    24. PS1 deficiency depletes vATPase, impairing lysosomal acidification and calcium homeostasis. PMID: 26299959
    25. Abeta oligomers in the cerebrospinal fluid (CSF) further promoted the expression of APH-1alpha/-1beta (by >2.5-fold), which enhances the gamma-cleavage of APP and Abeta deposition during AD progression PMID: 26293690
    26. This study presents novel evidence for the differential expression of PS proteins in a nongenetic model for aging, resulting in an overall increase of the PS2 to PS1 ratio. PMID: 25204494
    27. Alzheimer's disease-causing proline substitutions lead to presenilin 1 aggregation and malfunction. PMID: 26438723
    28. SLC2A13 is a novel gamma-secretase associated protein that regulates amyloid beta production without affecting Notch cleavage. PMID: 26094765
    29. PS1 may affect neuroprotection and Alzheimer disease by controlling survival signaling of neuronal EGFR. PMID: 25985800
    30. This study demonstrates that p300 mediates histone acetylation of PS1 and BACE1 in a cellular model of Alzheimer's disease. PMID: 25051175
    31. Liraglutide restores cerebral, splenic, and renal architecture in APP/PS1 mice. PMID: 25556713
    32. Early hyperinsulinemia is enough to exacerbate Alzheimer pathology observed in APP/PS1 mice, and supports the role of insulin-resistance therapies to stop or delay central complications associated. PMID: 24998414
    33. Results demonstrate that APP, Bace1 and PS1, accumulate acutely in an experimental model of spinal cord injury; inhibiting Abeta production by targeting the APP secretases results in greater white matter loss and impaired functional recovery PMID: 24630972
    34. It influences processing of the acetylcholinesterase membrane anchor PRiMA. PMID: 24612677
    35. This is the first study to provide a reference proteome map for splenocyte populations in Abeta/presenilin-1 double transgenic mice which will be helpful for future studies focused on understanding peripheral changes in this model. PMID: 24293237
    36. Thus study showed a significant reduction in the fraction of mushroom spines in hippocampal neurons from the presenilin-1 M146V knockin (KI) mouse model of familial alzheimer disease. PMID: 24698269
    37. PSEN-1 cleavage of ErbB4 for non-canonical signaling through 4ICD release does not require prior cleavage by TACE. PMID: 24462774
    38. At the transcriptional level, Psen1/2 removal induced cyclic AMP response element-binding protein (CREB)/CREB-binding protein binding. PMID: 24145027
    39. White matter changes across the whole brain of an amyloid precursor protein/presenilin 1 (APP/PS1) mouse model, were investigated. PMID: 23877934
    40. TRAF6-deficiency leads to a reduction in presenilin protein levels and reduced PS1 ubiquitination. PMID: 23707529
    41. the PS1/gamma-secretase system regulates release of glutamate, tyrosine phosphorylation, and surface expression of GluN2B-containing NMDARs. PMID: 24025330
    42. mechanism by which PS regulates synaptic function and calcium homeostasis using acute hippocampal slices from PS1 and PS2 conditional knockouts and primary cultured postnatal hippocampal neurons PMID: 23918386
    43. data delineate a promoter responsive element targeted by parkin that drives differential regulation of presenilin-1 and presenilin-2 transcription with functional consequences for gamma-secretase activity and cell death. PMID: 23359614
    44. Spatial learning and memory, fear conditioning, and sensorimotor gating were unaffected in APP-PS1 transgenic mice PMID: 23419740
    45. Pathogenic PSEN1 mutations suggest dominant-negative inhibition of presenilin activity which plays a role in Alzheimer's disease pathogenesis. PMID: 23843529
    46. Tthe loss of PS in PS knockout MEFs results in severely blunted PML expression and attenuated cell death upon camptothecin exposure. PMID: 23306558
    47. Postnatally, presenilin 1 mRNA is upregulated from postnatal day 45 and thereafter downregulated at 20 weeks. PMID: 22796216
    48. These data indicate that alterations in lysosomal calcium in the absence of presenilins might be leading to disruptions in autophagy. PMID: 23103503
    49. Reintroduction of PS1 into PS1-/- endothelial cells led to a progressive decrease in fibronectin levels showing that the increased fibronectin in PS1-/- endothelial cells was due to loss of PS1. PMID: 23259730
    50. our results suggest a direct role for PSEN in the regulation of the oxygen sensing pathway via the APP/AICD cleavage cascade. PMID: 23365231

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  • 亚细胞定位：
    Endoplasmic reticulum. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic granule. Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle. Cell projection, growth cone. Cell projection, neuron projection. Early endosome. Early endosome membrane; Multi-pass membrane protein. Cell projection, axon. Cell junction, synapse.
  • 蛋白家族：
    Peptidase A22A family
  • 组织特异性：
    Detected in embryonic brain. Detected in adult skin epidermis (at protein level).
  • 数据库链接：

    KEGG: mmu:19164

    STRING: 10090.ENSMUSP00000048363

    UniGene: Mm.998