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  4. Recombinant Mouse Podocalyxin (Podxl), partial

Recombinant Mouse Podocalyxin (Podxl), partial

  • 中文名称：
    小鼠Podxl重组蛋白
  • 货号：
    CSB-YP865629MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Podxl重组蛋白
  • 货号：
    CSB-EP865629MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Podxl重组蛋白
  • 货号：
    CSB-EP865629MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Podxl重组蛋白
  • 货号：
    CSB-BP865629MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Podxl重组蛋白
  • 货号：
    CSB-MP865629MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Podxl; Pclp1; Podocalyxin; Podocalyxin-like protein 1; PC; PCLP-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
  • 基因功能参考文献：
    1. Podocalyxin-like protein 1 is a relevant marker for human c-kit(pos) cardiac stem cells.( PMID: 23897803
    2. Podxl-KO led to heightened G-CSF activation of Rap1a(GTP), and Rap1a(GTP) inhibition attenuated Podxl-KO neutrophil migration. Studies have revealed novel roles for Podxl as an important modulator of neutrophil and monocyte formation and of Rap1a activation during stress hematopoiesis. PMID: 28408238
    3. Podxl-overexpression in neural stem/progenitor cells leads to an up-regulation of Annexin A2. PMID: 27470587
    4. when endogenous podocalyxin was removed from highly metastatic 4T1 mammary tumor cells there was a decrease in collective invasion . Podocalyxin is a tumor cell-intrinsic regulator of experimental collective tumor cell invasion and tumor budding. PMID: 26796961
    5. Podocalyxin has a role in glioblastoma multiforme cell invasion and proliferation via beta-catenin signaling PMID: 25349993
    6. Loss of podocalyxin from the lung vasculature results in changes to the lung structure and function including increased lung volume when inflated under constant pressure (25 cm H2O) and changes to the matrix composition. PMID: 25303643
    7. CTGF is an important mediator of high glucose-induced podocyte damage and decreases the protein level of podocalyxin by the ERK1/2 pathway. PMID: 21602138
    8. platelet Podxl is involved in the control of hemostasis acting as a platelet co-stimulator, likely due to its pro-adhesive properties PMID: 22016802
    9. Full-length recombinant murine podocalyxin recruits NHERF-1 to the apical domain. Ectopic expression in human & canine epithelial cells leads to microvilli formation on an apical domain extending laterally to the junctional complexes. PMID: 17311105
    10. Podocalyxin plays multiple roles in neural development PMID: 20706633
    11. PODXL may act as a costimulator of agonists in the activation of platelets and formation of a stable thrombus. PMID: 20223501
    12. Data show that in podocalyxin-deficient mice, the thickness of the main carotid artery is significantly increased, and there is an increased number of choroidal capillaries lining the ventricular spaces. PMID: 19837166
    13. Podocalyxin mRNA was widely expressed at least from E14, the first age we studied, and expression remained high until adulthood PMID: 15661640
    14. In mice expressing an Erythropoietin receptor allele, compromised erythropoietin-induced podocalyxin expression correlated with enucleated red cells in bone marrow. PMID: 17403918
    15. PODXL(hi)/CD49f(hi) MSCs were less prone to produce lethal pulmonary emboli PMID: 18818395
    16. Podocalyxin selectively marks erythroid-committed progenitors during anemic stress but is dispensable for efficient recovery. PMID: 19004540
    17. Podocalyxin is required for normal kidney function PMID: 11435469

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  • 亚细胞定位：
    Apical cell membrane. Cell projection, microvillus. Membrane raft. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Podocalyxin family
  • 组织特异性：
    Expressed in liver cells and hematopoietic cells (at protein level). Glomerular epithelium cell (podocyte).
  • 数据库链接：

    KEGG: mmu:27205

    STRING: 10090.ENSMUSP00000026698

    UniGene: Mm.89918