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  4. Recombinant Mouse Platelet glycoprotein VI (Gp6), partial

Recombinant Mouse Platelet glycoprotein VI (Gp6), partial

  • 中文名称：
    小鼠Gp6重组蛋白
  • 货号：
    CSB-EP009689MO1
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Gp6
  • Uniprot No.：
  • 别名：
    Gp6; Platelet glycoprotein VI; GPVI; Glycoprotein 5
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Extracellular Domain
  • 来源：
    E.coli
  • 分子量：
    43.0 kDa
  • 表达区域：
    22-265aa
  • 氨基酸序列
    QSGPLPKPSLQAQPSSLVPLGQSVILRCQGPPDVDLYRLEKLKPEKYEDQDFLFIPTMERSNAGRYRCSYQNGSHWSLPSDQLELIATGVYAKPSLSAHPSSAVPQGRDVTLKCQSPYSFDEFVLYKEGDTGSYKRPEKWYRANFPIITVTAAHSGTYRCYSFSSSSPYLWSAPSDPLVLVVTGLSATPSQVPTEESFPVTESSRRPSILPTNKISTTEKPMNITASPEGLSPPFGFAHQHYAK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Collagen receptor involved in collagen-induced platelet adhesion and activation. Plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma-chain, the Src kinases (likely FYN or LYN) and SYK, the adapter protein LAT and leads to the activation of PLCG2.
  • 基因功能参考文献：
    1. Platelet-specific deletion of GPVI does not lead to enhanced systemic inflammation and accelerated organ injury in two mouse models of sepsis PMID: 29269852
    2. These results reveal a novel and unexpected function of hepatic Fc-gamma-RIIB in the targeted downregulation of GPVI in vivo. PMID: 27297794
    3. collagen-I-mediated inhibition of proplatelet formation is specifically controlled by GPVI. PMID: 27505889
    4. platelet GP6 and thromboxane A2 receptor have a role in promoting inflammatory macrophage phenotype in skin inflammation PMID: 27818280
    5. Novel antithrombotic peptides derived from trowaglerix that acts through GPVI antagonism with greater safety-no severe bleeding. PMID: 28596377
    6. TULA-2 Protein Phosphatase Suppresses Activation of Syk through the GPVI Platelet Receptor for Collagen by Dephosphorylating Tyr(P)346, a Regulatory Site of Syk. PMID: 27609517
    7. Inhibition of platelet activation by an anti-GPVI antibody significantly reduces infarct size. PMID: 26916731
    8. These results demonstrate that GPVI is a receptor for fibrin and provide evidence that this interaction contributes to thrombus growth and stability. PMID: 26282541
    9. data demonstrate that genetic deletion of GPVI receptor, FcRgamma chain, or the alpha2beta1 integrin changes the thrombotic potentials of these platelets to collagen dependent on the stimulus mechanism. PMID: 25415203
    10. data suggest a novel role for FAK in GPVI-dependent ROS formation and platelet activation and elucidate a proximal signaling role for FAK within the GPVI pathway. PMID: 25415317
    11. our results show that GPVI plays a dual role in inflammation by enhancing neutrophil-damaging activities while supporting the activation and hemostatic adhesion of single platelets to neutrophil-induced vascular breaches. PMID: 26036804
    12. This study identifies GPVI as a platelet receptor for polymerized fibrin with 2 major functions: (1) amplification of thrombin generation and (2) recruitment of circulating platelets to clots. PMID: 25977585
    13. Functional studies of platelets from Ceacam2(-/-)-deficient mice (Cc2(-/-)) revealed that CEACAM2 serves to negatively regulate collagen glycoprotein VI (platelet) (GPVI)-FcRgamma-chain and the C-type lectinlike receptor 2 (CLEC-2) signaling PMID: 25085348
    14. Glaucocalyxin A inhibits platelet activation and thrombus formation preferentially via GPVI signaling pathway. PMID: 24386454
    15. a delayed and markedly reduced thrombogenic response was still evident in VWF(-/-), GPVI, and alpha2beta1 blocked animals, suggesting that alternative primary hemostatic mechanisms can partially rescue the bleeding phenotype associated with these defects. PMID: 25051961
    16. RhoG is expressed and activated in platelets, plays an important role in GPVI-Fc receptor gamma-chain complex-mediated platelet activation, and is critical for thrombus formation in vivo. PMID: 24106269
    17. demonstrate that isolated targeting of either GPVI or CLEC-2 in vivo does not affect expression or function of the respective other receptor PMID: 23448972
    18. Data show an inhibitory function of CLP-36 in GPVI immunoreceptor tyrosine-based activation motif signaling and as a key regulator of arterial thrombosis. PMID: 22955732
    19. Recombinant GPVI-Fc immunoglobulin fragment binds to activated vascular endothelium and prevents platelet/endothelial interaction. PMID: 22814400
    20. Cdc42 is required for platelet filopodia formation, secretion and aggregation and therefore plays a critical role in platelet mediated hemostasis and thrombosis. PMID: 21789221
    21. A model in which PECAM-1/SHP-2 complexes, formed in a Lyn-dependent manner, suppress GPVI signaling. PMID: 21297004
    22. PECAM-1-mediated inhibition of GPVI-dependent platelet responses result from recruitment of SHP-2-p85 complexes to tyrosine-phosphorylated PECAM-1, which diminishes the association of PI3K with activatory signaling molecules Gab1 and LAT PMID: 20723025
    23. Glycoprotein 6 cleavage in vitro can occur independently through either ADAM10 or ADAM17 in response to distinct stimuli. PMID: 20644114
    24. Ablation of TULA-2 resulted in hyperphosphorylation of Syk and its downstream effector phospholipase C-gamma2 as well as enhanced platelet glycoprotein VI-mediated platelet functional responses. PMID: 20585042
    25. The presence of platelet GPVI facilitates experimental tumor metastasis but does not contribute to the growth of primary tumors. PMID: 19624454
    26. the GPVI receptor utilizes a unique intracellular proline-rich domain (PRD) to accelerate platelet activation, a requirement for efficient platelet adhesion to collagen under flow PMID: 19940238
    27. c-Cbl negatively regulates platelet responses to GpVI agonists and to thrombin, with the latter effect possibly being mediated downstream of GpIIb/IIIa PMID: 14629478
    28. Platelet mitochondrial injury induced rapid proteolytic cleavage of GPVI and GPIb; platelet stimulation with thrombin or CRP, however, resulted in marked metalloproteinase-dependent shedding of GPIbalpha, but not GPVI. PMID: 15116256
    29. studies establish platelet-collagen responses under physiologic flow as the consequence of a close partnership between 2 structurally distinct receptors, glycoprotein VI and integrin alpha2beta1 PMID: 15886326
    30. study demonstrates a minor role for the p110delta catalytic subunit in mediating platelet activation by the collagen receptor GPVI and integrin alphaIIbeta3 PMID: 16011964
    31. These observations clearly establish that blockade of GPVI may attenuate platelet-collagen interactions without adversely affecting the bleeding time. PMID: 16139873
    32. GPVI is a novel receptor for laminin and support a model in which integrin alpha6beta1 brings laminin to GPVI. PMID: 16219796
    33. Platelet activation by thrombin appears to be more important after laser injury than platelet activation by GPVI-collagen. PMID: 16455953
    34. Absence of GP Ibalpha function has a more profound antithrombotic effect in vivo than absence of the GP VI-dependent pathway of collagen-induced adhesion/activation PMID: 16961609
    35. activation of phospholipase C gamma 2 via GPVI is dependent on 2 complementary events PMID: 17579183
    36. one or more modifier genes in Mh control the extent to which in vivo platelet thrombus formation is disrupted by the absence of platelet GPVI PMID: 17991808
    37. Globular adiponectin induces platelet activation through the collagen receptor GPVI-Fc receptor gamma chain complex PMID: 18419742
    38. In mice, GPVI-mediated platelet adhesion to the atherosclerotic vascular wall is involved in atheroprogression in vivo. PMID: 18431526
    39. GPVI-Fc preferentially bound to sites of vascular injury and was able to inhibit neointima formation after wire-induced vascular injury in ApoE(-/-) mice. PMID: 18566102
    40. Gads plays a key role in linking the adapter LAT to SLP-76 in response to weak activation of GPVI and CLEC-2 whereas LAT is required for full activation over a wider range of agonist concentrations. PMID: 18826392
    41. demonstrate a reciprocal relationship in levels of the novel PKC isoforms delta and epsilon in human and mouse platelets and a selective role for PKCepsilon in signalling through GPVI. PMID: 19030108
    42. PI3Kbeta plays an essential role in GPVI-mediated platelet aggregation and Akt activation PMID: 19700402

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  • 亚细胞定位：
    Cell membrane; Single-pass membrane protein.
  • 组织特异性：
    Megakaryocytes and platelets.
  • 数据库链接：

    KEGG: mmu:243816

    STRING: 10090.ENSMUSP00000104231

    UniGene: Mm.248662