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  4. Recombinant Mouse Peroxisome proliferator-activated receptor gamma (Pparg)

Recombinant Mouse Peroxisome proliferator-activated receptor gamma (Pparg)

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  • 中文名称：
    小鼠Pparg重组蛋白
  • 货号：
    CSB-EP018424MOb0
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pparg; Nr1c3; Peroxisome proliferator-activated receptor gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    63.3 kDa
  • 表达区域：
    1-505aa
  • 氨基酸序列
    MGETLGDSPVDPEHGAFADALPMSTSQEITMVDTEMPFWPTNFGISSVDLSVMEDHSHSFDIKPFTTVDFSSISAPHYEDIPFTRADPMVADYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNRPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKNIPGFINLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKVLQKMTDLRQIVTEHVQLLHVIKKTETDMSLHPLLQEIYKDLY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels.
  • 基因功能参考文献：
    1. these studies identify a PPARgamma-dependent miR-424/503-CD40 signaling axis that is critical for regulation of inflammation mediated angiogenesis PMID: 28566713
    2. WISP1 interacts with PPARgamma and that this interaction results in the inhibition of PPARgamma activity. T PMID: 28496206
    3. manipulation of PPAR-gamma activity has the potential to balance lipid-induced M1/M2 macrophage/Kupffer cell polarization. PMID: 28300213
    4. we identified IRF6 as a novel PPARgamma co-suppressor that serves a key role in suppressing PPARgamma-mediated cerebrovascular endothelial cytoprotection following ischemia. PMID: 28526834
    5. Data show that a peroxisome proliferator activated receptor gamma (PPARgamma)-dependent adipogenic response regulates muscle fat infiltration during regeneration. PMID: 30011852
    6. High fat diet-induced obesity exacerbates hematopoiesis deficiency and cytopenia caused by 5-fluorouracil via peroxisome proliferator-activated receptor gamma. PMID: 29305999
    7. findings suggest that MCAM is a gene upregulated and involved in maintaining PPARgamma induction in the late but not in the early stages of 3T3-L1 fibroblasts adipogenesis. PMID: 29468504
    8. Results suggest that hypothalamic peroxisome proliferator-activated receptor-gamma plays a vital role in ghrelin production and food intake in mice. PMID: 29655655
    9. Data suggest that expression of microRNA-128-3p is down-regulated during adipogenesis; an abundance of microRNA-128-3p appears to down-regulate adipogenesis and up-regulate lipolysis in adipocytes by targeting expression of Pparg (peroxisome proliferator-activated receptor gamma) and Sertad2 (SERTA domain-containing protein-2). PMID: 29654510
    10. The present study suggests for the first time that increased PPAR-gmma expression by high fat diet is responsible for cardiac dysfunction via upregulation of mitochondrial enzymes HMGCS2, BDH1 and PDK4. PMID: 30048968
    11. Data suggests that exposure to vitamin D deficiency during perinatal period directly affects expression of genes involved in development of adipose tissue in non-obese offspring; expression levels of Pparg (peroxisome proliferator activated receptor gamma) and Vdr (vitamin D receptor) are up-regulated in adipose tissue of male offspring. PMID: 28004271
    12. Our results found that, in the mice with T2D and AD, the activators of PPARg/AMPK signaling pathway significantly increased the expression level of IDE, and decreased the accumulation of Ab40 and Ab42, as well as alleviated the spatial learning and recognition impairments. PMID: 29222348
    13. PPARgamma functions as a checkpoint, guarding against inflammation, and is permissive for alternative activation of macrophages by facilitating glutamine metabolism PMID: 30006480
    14. Inhibition of this phosphorylation results in deregulation of p53 signaling, and biochemical studies show that PPARgamma physically interacts with p53 in a manner dependent on S273 phosphorylation. PMID: 29295932
    15. Direct regulation of mitochondrially encoded electron transport chain gene expression by mitochondrial PPARgamma2, in part, underlies the isoform-specific role for PPARgamma2 in brown adipocytes. PMID: 29566074
    16. L-Carnitine alleviated epithelial mesenchymal transformation-associated renal fibrosis caused by perfluorooctanesulfonate through a Sirt1- and PPARgamma-dependent mechanism. PMID: 28973641
    17. Pgc-1beta (-/-) hearts show pro-arrhythmic instabilities attributable to altered action potential conduction and activation rather than recovery characteristics. PMID: 28821956
    18. CACUL1 reciprocally regulates SIRT1 and LSD1 to repress PPARgamma and inhibit adipogenesis. PMID: 29233982
    19. results support a stimulatory effect of Pb on adipogenesis which involves ERK activation and C/EBPbeta upregulation prior to PPARgamma and adipogenesis activation. PMID: 28646352
    20. Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE(-/-) /LDLR(-/-) mouse via a PPARgamma/CD36 pathway. PMID: 28631352
    21. demonstrate that chronic ethanol ingestion activates peroxisome proliferator-activated receptor gamma (PPARgamma) and its target gene, monoacylglycerol O-acyltransferase 1 (MGAT1) PMID: 27404390
    22. The present results indicated that PPARgamma may serve a protective role on bEnd.3 cells and that BIRC5 may be a downstream target of PPARgamma regulation during cerebral ischemia. PMID: 29039513
    23. this study shows that Osterix represses adipogenesis by negatively regulating PPARgamma transcriptional activity PMID: 27752121
    24. HDAC3 inhibition in particular enhanced PPARgamma acetylation, prevented Klotho loss, and consequentially attenuated renal damage in mice model of chronic kidney disease. PMID: 28416226
    25. additional transgenic mouse PPAR-gamma or pharmacological activation of PPAR-gamma effectively prevented transgenic mouse DNMT1-induced proinflammatory cytokine production in macrophages and atherosclerosis development in the mouse model. PMID: 27530451
    26. data demonstrated that reduction of Pparg expression in T-helper cells is critical for spontaneous SLE-like autoimmune disease development; we also revealed a novel function of PPARgamma in lymphocyte trafficking and cross talk between Th17 and B cells. PMID: 27221351
    27. the extracts dramatically attenuated the levels of adipogenic transcriptional factors, including CCAAT enhancer-binding protein alpha (C/EBPa), CCAAT enhancer-binding protein beta (C/EBPb), and gamma receptors by peroxisome proliferators (PPARg), during adipogenesis PMID: 28604636
    28. these findings identified an important role of renal tubular epithelium-targeted PPAR-gamma in maintaining the normal epithelial phenotype and opposing fibrogenesis, possibly via antagonizing oxidative stress PMID: 27602490
    29. Activation of PPARgamma in hematopoietic stem cells impaired hematopoietic repopulation. PPARgamma inhibition by shRNA or chemical compounds significantly improves the repopulating ability of Fancd2-/- HSCs. PMID: 28416286
    30. Study found that the metabolic master regulator PGC-1alpha is differentially affected by ALS-associated mutations in brain vs. peripheral tissues. Increased PGC-1alpha activity in peripheral tissue contributes to the metabolic phenotype, while in the CNS blunting of the PGC-1alpha response renders motoneurons vulnerable. PMID: 27818323
    31. DBZ is a putative PPARgamma agonist that prevents HFD-induced obesity-related metabolic syndrome and reverse gut dysbiosis. DBZ may be used as a beneficial probiotic agent to improve HFD-induced obesity-related metabolic syndrome in obese individuals PMID: 28736228
    32. Altogether, the s demonstrate that Dnmt3a and Dnmt3b protect the epidermis from tumorigenesis and that squamous carcinomas are sensitive to inhibition of PPAR-gamma. PMID: 28425913
    33. FBXO9 directly interacted with PPAR gamma through the activation function-1 domain and ligand-binding domain. FBXO9 decreased the protein stability of PPAR gamma through induction of ubiquitination. PMID: 27197753
    34. inhibition of Hsp90 in Sec61a1 mutant hepatocytes also reduced Ppargamma protein levels and signaling. PMID: 24927728
    35. TET proteins, particularly TET2, were required for adipogenesis by modulating DNA methylation at the Ppargamma locus, subsequently by inducing Ppargamma gene expression. PMID: 28100914
    36. ATIP plays an important role in AT2 receptor-mediated PPARgamma activation. PMID: 26471325
    37. Our data showed that besides the high parasite burden and lack of microbicidal molecules, an imbalance with high COX-2 and 5-LOX eicosanoid expression and a lack of regulatory PPAR-gamma cytoplasm-to-nucleus translocation in macrophages were observed in mice that develop cerebral malaria. PMID: 27887739
    38. These results uncover a murine hepatic steatosis regulatory axis consisting of ABL1-PPARgamma2-MLL4, which may serve as a target of anti-steatosis drug development. PMID: 27806304
    39. Data suggest that a dietary factor, dietary supplement conjugated linoleic acid, improves endurance capacity of skeletal muscles independent of mild-intensity exercise/conditioning via Pparg-mediated mechanisms involved in gene expression regulation. PMID: 27736732
    40. madecassic acid was the active form of madecassoside in ameliorating colitis by restoring the Th17/Treg balance via regulating the PPARgamma/AMPK/ACC1 pathway. PMID: 28358365
    41. Pin1 enhances adipocyte differentiation by regulating the function of PPARgamma. PMID: 27475846
    42. The s report that macrophage PPARgamma deletion in mice not only exacerbates mammary tumor development but also impairs the anti-tumor effects of rosiglitazone. Mechanistically, the s identify Gpr132 as a novel direct PPARgamma target in macrophage whose expression is enhanced by PPARgamma loss but repressed by PPARgamma activation. PMID: 27692066
    43. Data indicate that obesity-induced insulin resistance and lipotoxicity can be treated with ginsenoside Rg3, which acts though the STAT5-PPAR gamma pathway in vivo and in vitro. PMID: 29042402
    44. TAK1 is required for PPARgamma transactivation and promotes PPARgamma transcriptional activity synergistically with TAK1 binding protein 1 (TAB1). PMID: 27293199
    45. PPAR gamma role in fat deposition and body weight gain.PPAR gamma is regulated by miR-27b. PMID: 28943435
    46. Time course analysis demonstrated that the adipogenic 'hub', sampled by PPARgamma and Lpin1, undergoes orchestrated reorganization during adipogenesis. PMID: 28755519
    47. Aleglitazar protects cardiomyocytes against hyperglycaemia-induced apoptosis by combined activation of both peroxisome proliferator-activated receptor-alpha and peroxisome proliferator-activated receptor-gamma. PMID: 28111985
    48. IRF6 suppresses PPARgamma through binding IRF recognition sites located upstream of the PPARgamma coding region. Taken together, the results suggest that an IRF6/PPARgamma regulatory axis suppresses anti-inflammatory responses in bone marrow-derived macrophages and provides references for future study addressing dysregulated metabolic and immunologic homeostasis of obese adipose tissue. PMID: 28645193
    49. adipogenic miR-27a in adipose tissue upregulates macrophage activation via inhibiting PPARgamma of insulin resistance induced by high-fat diet-associated obesity PMID: 28365247
    50. Report shows the identification of a novel Pparg splicing variant, Pparc1sv, in mice that is synergistically upregulated with Pparc2 during adipocyte differentiation of 3T3-L1 cells and mouse primary cultured preadipocytes. Both promotors are activated by C/EBPbeta and C/EBPdelta. PMID: 23840343

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Nuclear hormone receptor family, NR1 subfamily
  • 组织特异性：
    Highest expression in white and brown adipose tissue. Also found in liver, skeletal muscle, heart, adrenal gland, spleen, kidney and intestine. Isoform 2 is more abundant than isoform 1 in adipose tissue.
  • 数据库链接：

    KEGG: mmu:19016

    STRING: 10090.ENSMUSP00000000450

    UniGene: Mm.3020